Fusion of uniluminal vascular spheroids: A model for assembly of blood vessels
Open accessAbstract
We evaluated the self‐assembly properties of uniluminal vascular spheroids having outer layers of vascular smooth muscle cells and a contiguous inner layer of endothelial cells lining a central lumen. We showed that while pairs of uniluminal vascular spheroids suspended in culture medium fused to form a larger diameter spheroidal structure, spheroids in collagen hydrogels formed elongated structures. These findings highlight the potential use of uniluminal vascular spheroids as modules to engineer blood vessels. We also demonstrate that uniluminal vascular spheroid fusion conforms to models describing the coalescence of liquid drops. Furthermore, the fusion of uniluminal vascular spheroids in vitro closely resembled the in vivo process by which the descending aorta forms from the fusion of the paired dorsal aortae during embryonic development. Together, the findings indicate that tissue liquidity underlies uniluminal vascular spheroid fusion and that in vivo anastomosis of blood vessels may involve a similar mechanism. Developmental Dynamics 239:398–406, 2010. © 2009 Wiley‐Liss, Inc.
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Spheroids display liquid-like behavior and undergo coalescence similar to liquid droplets, explained by the differential adhesion hypothesis where cells rearrange to maximize adhesive cadherin bonding and reduce free energy.
“Spheroids display liquid-like behavior and undergo coalescence in a similar manner to liquid droplets37, which has been explained using the differential adhesion hypothesis where cells rearrange to maximize adhesive cadherin bonding and reduce free energy38.”
From 3D bioprinting of high cell-density heterogeneous tissue models through spheroid fusion within self-healing hydrogels (2021) · cites this paper as [37]
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- Authors
- Fleming, P. A., Argraves, W. S., Gentile, C., Neagu, A., Forgacs, G., & Drake, C. J.
- Affiliations
- Medical University of South Carolina (US)
- Victor Babeș University of Medicine and Pharmacy Timișoara (RO)
- Corresponding author
- Drake, Christopher J.
- Venue
- Developmental Dynamics
- Publisher
- Wiley
- Published
- 2010-01-22
- Volume
- 239
- Issue
- 2
- Pages
- 398-406
- ISSN
- 1058-8388 (ISSN-L)
- 1097-0177
- PMC
- PMC3176722
- OpenAlex
- W2091452743
- Cited by
- 123
- References
- 48
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 6 · 2023: 11 · 2022: 9 · 2021: 16 · 2020: 10 · 2019: 6 · 2018: 7 · 2017: 16 · 2016: 7 · 2015: 6 · 2014: 5 · 2013: 5 · 2012: 8
- FWCI
- 4.06
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Developmental Dynamics (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Science Foundation (0244045, 0526854)
- Adobe Systems
- National Institutes of Health (HL095067)
- Medical Research Council
- National Heart, Lung, and Blood Institute (R01HL080168, R01HL057375, HL061873, R01HL095067, HL 57375, R01HL061873, HL80168, HL095067)
- Division of Materials Research
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- spheroid fusion, vascular smooth muscle cells, endothelial cells, collagen gel, droplet coalescence, aortic development, vascular anastomosis
- MeSH terms
- Animals, Aorta, Blood Vessels, Cell Fusion, Collagen, Female, Models, Cardiovascular, Pregnancy, Rabbits, Spheroids, Cellular, Hydrogels, Tissue Engineering, Mice, Rats
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