Cited by 123Tissue Engineering and Regenerative Medicine3D Printing in Biomedical ResearchCellular Mechanics and Interactions

Fusion of uniluminal vascular spheroids: A model for assembly of blood vessels

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Fleming, Paul A., Argraves, W. Scott, Gentile, Carmine, Neagu, Adrian, Forgacs, Gabor, Drake, Christopher J.·2010·Developmental Dynamics

Abstract

We evaluated the self‐assembly properties of uniluminal vascular spheroids having outer layers of vascular smooth muscle cells and a contiguous inner layer of endothelial cells lining a central lumen. We showed that while pairs of uniluminal vascular spheroids suspended in culture medium fused to form a larger diameter spheroidal structure, spheroids in collagen hydrogels formed elongated structures. These findings highlight the potential use of uniluminal vascular spheroids as modules to engineer blood vessels. We also demonstrate that uniluminal vascular spheroid fusion conforms to models describing the coalescence of liquid drops. Furthermore, the fusion of uniluminal vascular spheroids in vitro closely resembled the in vivo process by which the descending aorta forms from the fusion of the paired dorsal aortae during embryonic development. Together, the findings indicate that tissue liquidity underlies uniluminal vascular spheroid fusion and that in vivo anastomosis of blood vessels may involve a similar mechanism. Developmental Dynamics 239:398–406, 2010. © 2009 Wiley‐Liss, Inc.

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  • Spheroids display liquid-like behavior and undergo coalescence similar to liquid droplets, explained by the differential adhesion hypothesis where cells rearrange to maximize adhesive cadherin bonding and reduce free energy.

    “Spheroids display liquid-like behavior and undergo coalescence in a similar manner to liquid droplets37, which has been explained using the differential adhesion hypothesis where cells rearrange to maximize adhesive cadherin bonding and reduce free energy38.”

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Authors
Fleming, P. A., Argraves, W. S., Gentile, C., Neagu, A., Forgacs, G., & Drake, C. J.
Affiliations
  • Medical University of South Carolina (US)
  • Victor Babeș University of Medicine and Pharmacy Timișoara (RO)
Corresponding author
Drake, Christopher J.
Venue
Developmental Dynamics
Publisher
Wiley
Published
2010-01-22
Volume
239
Issue
2
Pages
398-406
ISSN
  • 1058-8388 (ISSN-L)
  • 1097-0177
OpenAlex
W2091452743
Cited by
123
References
48
Citations by year
2026: 1 · 2025: 6 · 2024: 6 · 2023: 11 · 2022: 9 · 2021: 16 · 2020: 10 · 2019: 6 · 2018: 7 · 2017: 16 · 2016: 7 · 2015: 6 · 2014: 5 · 2013: 5 · 2012: 8
FWCI
4.06
Type
journal article
Language
en
Open access
Locations
  • Developmental Dynamics (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Science Foundation (0244045, 0526854)
  • Adobe Systems
  • National Institutes of Health (HL095067)
  • Medical Research Council
  • National Heart, Lung, and Blood Institute (R01HL080168, R01HL057375, HL061873, R01HL095067, HL 57375, R01HL061873, HL80168, HL095067)
  • Division of Materials Research
Topics
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords
spheroid fusion, vascular smooth muscle cells, endothelial cells, collagen gel, droplet coalescence, aortic development, vascular anastomosis
MeSH terms
Animals, Aorta, Blood Vessels, Cell Fusion, Collagen, Female, Models, Cardiovascular, Pregnancy, Rabbits, Spheroids, Cellular, Hydrogels, Tissue Engineering, Mice, Rats

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