Transthyretin mouse transgenes direct RFP expression or Cre‐mediated recombination throughout the visceral endoderm
Open accessAbstract
Transthyretin (Ttr) is a thyroid hormone transport protein secreted by cells of the visceral yolk sac and fetal liver in developing embryos, and by hepatocytes and the choroid plexus epithelium of the brain in adult mice. Spatiotemporal localization of Ttr mRNA during embryogenesis suggested that Ttr regulatory elements might drive transgene expression throughout the visceral endoderm of early mouse embryos. We use Ttr cis‐regulatory elements to generate Ttr::RFP and Ttr::Cre strains of mice, driving red fluorescent protein (RFP) and a nuclear‐localized Cre recombinase, respectively. Visualization of RFP fluorescence in Ttr::RFP transgenics confirms reporter localization throughout the visceral endoderm in early embryos and in the visceral yolk sac and fetal liver of later stage embryos. Using both GFP‐based and LacZ‐based Cre reporter strains, we demonstrate that in Ttr::Cre transgenics, Cre‐mediated recombination occurs throughout the visceral endoderm. The Ttr::Cre strain can therefore be used as a tool for genetic modifications within the visceral endoderm lineage. genesis 47:447–455, 2009. © 2009 Wiley‐Liss, Inc.
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13ss (E8.75) is the latest stage at which the visceral endoderm versus definitive endoderm origin of gut tube cells can be unambiguously assigned using Afp-GFP or Ttr-Cre mouse lines.
“13ss (E8.75), approximately corresponding to midgestation, the latest stage analyzed in this study, is the latest stage for unambiguous assignment of visceral endoderm (VE)-vs.-definitive endoderm (DE) origin of gut tube cells using Afp-GFP7,12 or Ttr-Cre7,35 mouse lines.”
From The emergent landscape of the mouse gut endoderm at single-cell resolution (2019) · cites this paper as [35]
The ‘ADDITIONAL SITES OF RFP EXPRESSION AND Cre RECOMBINATION IN LATE STAGE EMBRYOS’ section says later transgene activity precludes further analysis of visceral-endoderm-derived cells in embryonic endoderm derivatives. It does not establish that 13 somites (E8.75) is the latest stage at which gut-tube cell origins can be unambiguously assigned using Afp-GFP or Ttr-Cre. Page numbers are unavailable in our copy. (LLM, conf=0.84)
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References 32 · Cited by 72
Research works printed but not linked: 31
- Beddington RS Robertson EJ 1999 Axis development and early asymmetry in mammals Cell 96 195 209 9988215
- Campbell RE Tour O Palmer AE Steinbach PA Baird GS Zacharias DA Tsien RY 2002 A monomeric red fluorescent protein Proc Natl Acad Sci USA 99 7877 7882 12060735 PMC122988
- Costa RH Van Dyke TA Yan C Kuo F Darnell JE Jr 1990 Similarities in transthyretin gene expression and differences in transcription factors: Liver and yolk sac compared to choroid plexus Proc Natl Acad Sci USA 87 6589 6593 2395861 PMC54582
- Downs KM Davies T 1993 Staging of gastrulating mouse embryos by morphological landmarks in the dissecting microscope Development 118 1255 1266 8269852
- Duncan SA Manova K Chen WS Hoodless P Weinstein DC Bachvarova RF Darnell JE Jr 1994 Expression of transcription factor HNF-4 in the extraembryonic endoderm, gut, and nephrogenic tissue of the developing mouse embryo: HNF-4 is a marker for primary endoderm in the implanting blastocyst Proc Natl Acad Sci USA 91 7598 7602 8052626 PMC44449
- Dziadek M 1978 Modulation of alphafetoprotein synthesis in the early postimplantation mouse embryo J Embryol Exp Morphol 46 135 146 81255
- Dziadek M Adamson E 1978 Localization and synthesis of alphafoeto-protein in post-implantation mouse embryos J Embryol Exp Morphol 43 289 313 75937
- Hayashi S Lewis P Pevny L McMahon AP 2002 Efficient gene modulation in mouse epiblast using a Sox2Cre transgenic mouse strain Mech Dev 119 Suppl 1 S97 S101 14516668
- Joyner AL Zervas M 2006 Genetic inducible fate mapping in mouse: Establishing genetic lineages and defining genetic neuroanatomy in the nervous system Dev Dyn 235 2376 2385 16871622
- Kwon GS Fraser ST Eakin GS Mangano M Isern J Sahr KE Hadjantonakis AK Baron MH 2006 Tg(Afp-GFP) expression marks primitive and definitive endoderm lineages during mouse development Dev Dyn 235 2549 2558 16708394 PMC1850385
- Lewandoski M 2001 Conditional control of gene expression in the mouse Nat Rev Genet 2 743 755 11584291
- Lewandoski M Wassarman KM Martin GR 1997 Zp3-cre, a transgenic mouse line for the activation or inactivation of loxP-flanked target genes specifically in the female germ line Curr Biol 7 148 151 9016703
- Long JZ Lackan CS Hadjantonakis AK 2005 Genetic and spectrally distinct in vivo imaging: Embryonic stem cells and mice with widespread expression of a monomeric red fluorescent protein BMC Biotechnol 5 20 15996270 PMC1192791
- Lu CC Brennan J Robertson EJ 2001 From fertilization to gastrulation: Axis formation in the mouse embryo Curr Opin Genet Dev 11 384 392 11448624
- Meehan RR Barlow DP Hill RE Hogan BL Hastie ND 1984 Pattern of serum protein gene expression in mouse visceral yolk sac and foetal liver EMBO J 3 1881 1885 6479150 PMC557612
- Mesnard D Filipe M Belo JA Zernicka-Goetz M 2004 The anterior-posterior axis emerges respecting the morphology of the mouse embryo that changes and aligns with the uterus before gastrulation Curr Biol 14 184 196 14761650
- Mesnard D Guzman-Ayala M Constam DB 2006 Nodal specifies embryonic visceral endoderm and sustains pluripotent cells in the epiblast before overt axial patterning Development 133 2497 2505 16728477
- Nagy A Gertsenstein M Vintersten K Behringer R 2003 Manipulating the mouse embryo: A laboratory manual Cold Spring Harbor, New York Cold Spring Harbor Laboratory Press
- Novak A Guo C Yang W Nagy A Lobe CG 2000 Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon Cre-mediated excision Genesis 28 147 155 11105057
- Papaioannou VE West JD 1981 Relationship between the parental origin of the X chromosomes, embryonic cell lineage and X chromosome expression in mice Genet Res 37 183 197 7262553
- Rodriguez TA Casey ES Harland RM Smith JC Beddington RS 2001 Distinct enhancer elements control Hex expression during gastrulation and early organogenesis Dev Biol 234 304 316 11397001
- Soprano DR Soprano KJ Goodman DS 1986 Retinol-binding protein and transthyretin mRNA levels in visceral yolk sac and liver during fetal development in the rat Proc Natl Acad Sci USA 83 7330 7334 3463972 PMC386710
- Soriano P 1999 Generalized lacZ expression with the ROSA26 Cre reporter strain Nat Genet 21 70 71 9916792
- Srinivas S 2006 The anterior visceral endoderm-turning heads Genesis 44 565 572 17078044
- Srinivas S Rodriguez T Clements M Smith JC Beddington RS 2004 Active cell migration drives the unilateral movements of the anterior visceral endoderm Development 131 1157 1164 14973277
- Tallquist MD Soriano P 2000 Epiblast-restricted Cre expression in MORE mice: A tool to distinguish embryonic vs. extra-embryonic gene function Genesis 26 113 115 10686601
- Tam PP Loebel DA 2007 Gene function in mouse embryogenesis: Get set for gastrulation Nat Rev Genet 8 368 381 17387317
- Vincent SD Robertson EJ 2004 Targeted insertion of an IRES Cre into the Hnf4α locus: Cre-mediated recombination in the liver, kidney, and gut epithelium Genesis 39 206 211 15282747
- Wakasugi S Maeda S Shimada K Nakashima H Migita S 1985 Structural comparisons between mouse and human prealbumin J Biochem 98 1707 1714 3005251
- Whittingham DG Wales RG 1969 Storage of two-cell mouse embryos in vitro Aust J Biol Sci 22 1065 1068 5374532
- Yan C Costa RH Darnell JE Jr Chen JD Van Dyke TA 1990 Distinct positive and negative elements control the limited hepatocyte and choroid plexus expression of transthyretin in transgenic mice EMBO J 9 869 878 1690125 PMC551747
- Authors
- Kwon, G. S., & Hadjantonakis, A.
- Affiliations
- Memorial Sloan Kettering Cancer Center (US)
- Corresponding author
- Hadjantonakis, Anna‐Katerina
- Venue
- genesis
- Publisher
- Wiley
- Published
- 2009-05-04
- Volume
- 47
- Issue
- 7
- Pages
- 447-455
- ISSN
- 1526-954X (ISSN-L)
- 1526-968X
- PMC
- PMC2878311
- OpenAlex
- W2120175640
- Cited by
- 72
- References
- 32
- Citations by year
- 2026: 4 · 2025: 1 · 2024: 3 · 2023: 3 · 2022: 1 · 2021: 3 · 2020: 4 · 2019: 3 · 2018: 3 · 2017: 5 · 2016: 1 · 2015: 4 · 2014: 6 · 2013: 9 · 2012: 6
- FWCI
- 2.96
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- genesis (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Memorial Sloan-Kettering Cancer Center
- National Cancer Institute (P30CA008748)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01HD052115)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Keywords
- prealbumin, visceral endoderm, visceral yolk sac, fetal liver, Cre-mediated recombination, transgenic reporter mice, mouse embryo development
- MeSH terms
- Red Fluorescent Protein, Animals, Endoderm, Luminescent Proteins, Prealbumin, Recombination, Genetic, Viscera, Transgenes, Integrases, Embryonic Development, Mice
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