The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Activation requirements for the induction of CD4+CD25+ T cell suppressor function
Abstract
The in vivo differentiation/survival of CD4+CD25+ T suppressor cells is dependent on IL‐2 and CD28‐mediated costimulatory signals. To determine the cytokine and costimulatory requirements for CD25+ T cells in vitro, we established a two‐stage culture system where CD25+ T cells were activated in a primary culture. In the subsequent culture, activated CD4+CD25+ cells were then mixed with responders in order to assess their suppressor function. Pre‐culture of CD25+ T cells with anti‐CD3 alone resulted in poor survival and minimal induction of suppressor activity. Pre‐culture in the presence of anti‐CD3 and IL‐2 or IL‐4, but not IL‐6, IL‐7, IL‐9, IL‐10 or IL‐15, resulted in proliferation of the CD25+ cells and induction of potent suppressor function. Inhibition of the interaction of CD28 or cytotoxic T lymphocyte‐associated antigen‐4 (CTLA‐4) with CD80/CD86 in the pre‐culture of CD4+CD25+ cells did not prevent the induction of suppressor function. Furthermore, the inhibition of costimulatory signals did not inhibit the ability of fresh CD25+ T cells to inhibit CD8+ responders under conditions where activation of the responders was independent of CD80/CD86. These studies support the view that activation of CD25+ T cells requires IL‐2/IL‐4 for their survival/differentiation into effector cells, but is independent of CD28/CTLA‐4‐mediated costimulation.
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Cites
A CD4+T-cell subset inhibits antigen-specific T-cell responses and prevents colitis
Groux, H., O'Garra, A., Bigler, M., Rouleau, M., Antonenko, S., de Vries, J. E., & Roncarolo, M. G. (1997) · Nature · doi:10.1038/39614 · Cited by 3,588
Thornton, A. M., & Shevach, E. M. (1998) · The Journal of Experimental Medicine · doi:10.1084/jem.188.2.287 · Cited by 2,639
Self-citationTakahashi, T., Tagami, T., Yamazaki, S., Uede, T., Shimizu, J., Sakaguchi, N., Mak, T. W., & Sakaguchi, S. (2000) · The Journal of Experimental Medicine · doi:10.1084/jem.192.2.303 · Cited by 2,233
Read, S., Malmström, V., & Powrie, F. (2000) · The Journal of Experimental Medicine · doi:10.1084/jem.192.2.295 · Cited by 2,205
Salomon, B., Lenschow, D. J., Rhee, L., Ashourian, N., Singh, B., Sharpe, A., & Bluestone, J. A. (2000) · Immunity · doi:10.1016/s1074-7613(00)80195-8 · Cited by 2,081
CD4+CD25+ regulatory T cells control Leishmania major persistence and immunity
Belkaid, Y., Piccirillo, C. A., Mendez, S., Shevach, E. M., & Sacks, D. L. (2002) · Nature · doi:10.1038/nature01152 · Cited by 1,725
Self-citationAsseman, C., Mauze, S., Leach, M. W., Coffman, R. L., & Powrie, F. (1999) · The Journal of Experimental Medicine · doi:10.1084/jem.190.7.995 · Cited by 1,615
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CD4+CD25+ Immunoregulatory T Cells
McHugh, R. S., Whitters, M. J., Piccirillo, C. A., Young, D. A., Shevach, E. M., Collins, M., & Byrne, M. C. (2002) · Immunity · doi:10.1016/s1074-7613(02)00280-7 · Cited by 1,395
Self-citationNatural versus adaptive regulatory T cells
Bluestone, J. A., & Abbas, A. K. (2003) · Nature Reviews Immunology · doi:10.1038/nri1032 · Cited by 1,323
Cited by
CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ T reg cells
Liu, W., Putnam, A. L., Xu-yu, Z., Szot, G. L., Lee, M. R., Zhu, S., Gottlieb, P. A., Kapranov, P., Gingeras, T. R., de St. Groth, B. F., et al. (2006) · The Journal of Experimental Medicine · doi:10.1084/jem.20060772 · Cited by 2,606
The PD‐1 pathway in tolerance and autoimmunity
Francisco, L. M., Sage, P. T., & Sharpe, A. H. (2010) · Immunological Reviews · doi:10.1111/j.1600-065x.2010.00923.x · Cited by 2,300
Mechanisms of Foxp3+ T Regulatory Cell-Mediated Suppression
Shevach, E. M. (2009) · Immunity · doi:10.1016/j.immuni.2009.04.010 · Cited by 1,754
Self-citationThornton, A. M., Korty, P. E., Tran, D. Q., Wohlfert, E. A., Murray, P. E., Belkaid, Y., & Shevach, E. M. (2010) · The Journal of Immunology · doi:10.4049/jimmunol.0904028 · Cited by 1,382
Self-citationPandiyan, P., Zheng, L., Ishihara, S., Reed, J., & Lenardo, M. J. (2007) · Nature Immunology · doi:10.1038/ni1536 · Cited by 1,286
Metadata
- Authors
- Thornton, A. M., Piccirillo, C. A., & Shevach, E. M.
- Venue
- European Journal of Immunology
- Publisher
- Wiley
- Published
- 2004-02
- Volume
- 34
- Issue
- 2
- Pages
- 366-376
- ISSN
- 0014-2980 (ISSN-L)
- 1521-4141
- OpenAlex
- W1971782694
- Cited by
- 296
- References
- 32
- FWCI
- 14.01
- Type
- journal article
- Language
- en
- Open access
- closed
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