The DOI matches its Crossref record; 56 of the 56 references it cites were found in a registry or an index.
Analysis of FOXP3 protein expression in human CD4+CD25+ regulatory T cells at the single-cell level
Open accessAbstract
The transcription factor FOXP3 plays a key role in CD4(+)CD25(+) regulatory T cell function and represents a specific marker for these cells. Despite its strong association with regulatory T cell function, in humans little is known about the frequency of CD4(+)CD25(+) cells that express FOXP3 protein nor the distribution of these cells in vivo. Here we report the characterization of seven anti-FOXP3 monoclonal antibodies enabling the detection of endogenous human FOXP3 protein by flow cytometry and immunohistochemistry. Flow-cytometric analysis showed that FOXP3 was expressed by the majority of CD4(+)CD25(high) T cells in peripheral blood. By contrast, less than half of the CD4(+)CD25(int) population were FOXP3(+), providing an explanation for observations in human T cells that regulatory activity is enriched within the CD4(+)CD25(high) pool. Although FOXP3 expression was primarily restricted to CD4(+)CD25(+) cells, it was induced following activation of both CD4(+) and CD8(+) T cell clones. These findings indicate that the frequency of FOXP3(+) cells correlates with the level of expression of CD25 in naturally arising regulatory T cells and that FOXP3 protein is expressed by some activated CD4(+) and CD8(+) T cell clones. These reagents represent valuable research tools to further investigate FOXP3 function and are applicable for routine clinical use.
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Cited by
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Transient expression of FOXP3 in human activated nonregulatory CD4 + T cells
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Metadata
- Authors
- Roncador, G., Brown, P. J., Maestre, L., Hue, S., Martínez-Torrecuadrada, J. L., Ling, K.-L., Pratap, S., Toms, C., Fox, B. C., Cerundolo, V., Powrie, F., & Banham, A. H.
- Venue
- European Journal of Immunology
- Publisher
- Wiley
- Published
- 2005-06
- Volume
- 35
- Issue
- 6
- Pages
- 1681-1691
- ISSN
- 0014-2980 (ISSN-L)
- 1521-4141
- OpenAlex
- W1998350666
- Cited by
- 592
- References
- 56
- FWCI
- 22.97
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Roncador, G., Brown, P. J., Maestre, L., Hue, S., Martínez-Torrecuadrada, J. L., Ling, K.-L., Pratap, S., Toms, C., Fox, B. C., Cerundolo, V., Powrie, F., & Banham, A. H. (2005). Analysis of FOXP3 protein expression in human CD4+CD25+ regulatory T cells at the single-cell level. European Journal of Immunology. https://doi.org/10.1002/eji.200526189
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