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Technical improvements in two‐dimensional electrophoresis increase the level of genetic variation detected in wheat‐seedling proteins
Abstract
The level of genetic variation revealed by two‐dimensional electrophoresis of proteins from seedlings of two wheat lines strongly depends on the technical procedures. Improvements in extraction and electrophoresis procedures relative to earlier experiments on the same material led to a significant increase in the genetic variation revealed: 15.2 % instead of 6.7 % of the spots were genetically variable. The improved procedure is based on (i) precipipation of proteins from wheat seedlings with trichloroacetic acid and acetone, (ii) solubilization of the proteins with a solution containing urea, potassium carbonate and sodium dodecyl sulfate, (iii) isoelectric focusing in an optimized pH gradient, obtained with a mixture of carrier ampholytes (Pharmalyte and Servalyt), and (iv) running elecrophoresis in the second dimension on gels with increased surface.
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Cited by
Chevallet, M., Santoni, V., Poinas, A., Rouquié, D., Fuchs, A., Kieffer, S., Kieffer, S., Lunardi, J., Garin, J., & Rabilloud, T. (1998) · ELECTROPHORESIS · doi:10.1002/elps.1150191108 · Cited by 331
Rogers, A., Fischer, B. U., Bryant, J., Frehner, M., Blum, H., Raines, C. A., & Long, S. P. (1998) · Plant Physiology · doi:10.1104/pp.118.2.683 · Cited by 203
Zörb, C., Schmitt, S., Neeb, A., Karl, S., Linder, M., & Schubert, S. (2004) · Plant Science · doi:10.1016/j.plantsci.2004.03.004 · Cited by 148
Barker, B. M., Kroll, K., Vödisch, M., Mazurie, A., Kniemeyer, O., & Cramer, R. A. (2012) · BMC Genomics · doi:10.1186/1471-2164-13-62 · Cited by 127
Comparative Proteomic Analysis of Brains of Naturally Aging Mice
Yang, S., Liu, T., Li, S., Zhang, X., Ding, Q., Que, H., Yan, X., Wei, K., & Liu, S. (2008) · Neuroscience · doi:10.1016/j.neuroscience.2008.04.012 · Cited by 82
Metadata
- Authors
- Damerval, C., De Vienne, D., Zivy, M., & Thiellement, H.
- Venue
- ELECTROPHORESIS
- Publisher
- Wiley
- Published
- 1986-01
- Volume
- 7
- Issue
- 1
- Pages
- 52-54
- ISSN
- 0173-0835 (ISSN-L)
- 1522-2683
- OpenAlex
- W2002605431
- Cited by
- 795
- References
- 0
- FWCI
- 17.54
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Technical improvements in two‐dimensional electrophoresis increase the level of genetic variation detected in wheat‐seedling proteins. https://app.cochat.ai/papers/10.1002/elps.1150070108
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- Damerval, C., De Vienne, D., Zivy, M., & Thiellement, H. (1986). Technical improvements in two‐dimensional electrophoresis increase the level of genetic variation detected in wheat‐seedling proteins. ELECTROPHORESIS. https://doi.org/10.1002/elps.1150070108
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