The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
New zwitterionic detergents improve the analysis of membrane proteins by two‐dimensional electrophoresis
Abstract
Severe quantitative loss of protein is often observed in high‐resolution two‐dimensional electrophoresis of membrane proteins, while the resolution is usually not affected. To improve the solubility of proteins in this technique, we tested denaturing cocktails containing various detergents and chaotropes. Best results were obtained with a denaturing solution containing urea, thiourea, and zwitterionic detergents, synthesized for this purpose. Among the dozen detergents synthesized and tested, amidosulfobetaines with an alkyl tail containing 14–16 carbons proved most efficient, solubilizing previously undetected membrane proteins.
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Cites
Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels
Shevchenko, A., Wilm, M., Vorm, O., & Mann, M. (1996) · Analytical Chemistry · doi:10.1021/ac950914h · Cited by 8,995
Damerval, C., De Vienne, D., Zivy, M., & Thiellement, H. (1986) · ELECTROPHORESIS · doi:10.1002/elps.1150070108 · Cited by 795
Rabilloud, T., Adessi, C., Giraudel, A., & Lunardi, J. (1997) · ELECTROPHORESIS · doi:10.1002/elps.1150180303 · Cited by 459
Self-citationRabilloud, T., Valette, C., & Lawrence, J. J. (1994) · ELECTROPHORESIS · doi:10.1002/elps.11501501223 · Cited by 366
Self-citationKammerloher, W., Fischer, U., Piechottka, G. P., & Schäffner, A. R. (1994) · The Plant Journal · doi:10.1046/j.1365-313x.1994.6020187.x · Cited by 337
Johansson, I., Larsson, C., Ek, B., & Kjellbom, P. O. (1996) · The Plant Cell · doi:10.1105/tpc.8.7.1181 · Cited by 304
Görg, A., Postel, W., Weser, J., Günther, S., Strahler, J. R., Hanash, S. M., & Somerlot, L. (1987) · ELECTROPHORESIS · doi:10.1002/elps.1150080207 · Cited by 269
Jahn, T., Fuglsang, A. T., Olsson, A., Brüntrup, I. M., Collinge, D. B., Volkmann, D., Sommarin, M., Palmgren, M. G., & Larsson, C. (1997) · The Plant Cell · doi:10.1105/tpc.9.10.1805 · Cited by 242
O'Connell, K. L., & Stults, J. T. (1997) · ELECTROPHORESIS · doi:10.1002/elps.1150180309 · Cited by 236
Proteome research: Complementarity and limitations with respect to the RNA and DNA worlds
Humphery‐Smith, I., Cordwell, S. J., & Blackstock, W. P. (1997) · ELECTROPHORESIS · doi:10.1002/elps.1150180804 · Cited by 215
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Bodzon-Kulakowska, A., Bierczynska-Krzysik, A., Dylag, T., Drabik, A., Suder, P., Noga, M., Jarzebinska, J., & Silberring, J. (2007) · Journal of Chromatography B · doi:10.1016/j.jchromb.2006.10.040 · Cited by 254
Proteomics Fingerprinting of Phagosome Maturation and Evidence for the Role of a Gα during Uptake
Gotthardt, D., Blancheteau, V., Bosserhoff, A., Ruppert, T., Delorenzi, M., & Soldati, T. (2006) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m600113-mcp200 · Cited by 98
Expression Profiling of Lymphocyte Plasma Membrane Proteins
Peirce, M. J., Wait, R., Begum, S., Saklatvala, J., & Cope, A. P. (2004) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m300064-mcp200 · Cited by 92
Metadata
- Authors
- Chevallet, M., Santoni, V., Poinas, A., Rouquié, D., Fuchs, A., Kieffer, S., Kieffer, S., Lunardi, J., Garin, J., & Rabilloud, T.
- Venue
- ELECTROPHORESIS
- Publisher
- Wiley
- Published
- 1998-08
- Volume
- 19
- Issue
- 11
- Pages
- 1901-1909
- ISSN
- 0173-0835 (ISSN-L)
- 1522-2683
- OpenAlex
- W2147604930
- Cited by
- 331
- References
- 19
- FWCI
- 15.56
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: New zwitterionic detergents improve the analysis of membrane proteins by two‐dimensional electrophoresis. https://app.cochat.ai/papers/10.1002/elps.1150191108
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- Chevallet, M., Santoni, V., Poinas, A., Rouquié, D., Fuchs, A., Kieffer, S., Kieffer, S., Lunardi, J., Garin, J., & Rabilloud, T. (1998). New zwitterionic detergents improve the analysis of membrane proteins by two‐dimensional electrophoresis. ELECTROPHORESIS. https://doi.org/10.1002/elps.1150191108
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
