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Improved silver staining procedure for fast staining in PhastSystem Development Unit. I. Staining of sodium dodecyl sulfate gels
Abstract
A new modification of silver staining of proteins in sodium dodecyl sulfate polyacrylamide gels is adapted to automated staining in PhastSystem Development Unit. The use of a reduction step, after fixation, with thiosulfate in alcoholic sodium acetate buffer results in a considerable increase in sensitivity without the need for a recycling step. The detection limit is tenfold lower than in the silver staining procedure recommended so far for PhastSystem and corresponds to 0.05–0.1 ng protein per band. Total staining time with the new procedure is 75 min.
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Cited by
Zabel, U., Schreck, R., & Baeuerle, P. A. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)52428-5 · Cited by 344
Behrendt, N., Rønne, E., Ploug, M., Petri, T., Løber, D., Nielsen, L. S., Schleuning, W. D., Blasi, F., Appella, E., & Danø, K. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)39348-2 · Cited by 274
Lind, D., Franken, S., Kappler, J., Jankowski, J., & Schilling, K. (2005) · Journal of Neuroscience Research · doi:10.1002/jnr.20354 · Cited by 222
BECKER, E., FLORIN, L., PFIZENMAIER, K., & KAUFMANN, H. (2008) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2008.03.008 · Cited by 86
Berndorfer, U., Wilms, H., & Herzog, V (1996) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.81.5.8626858 · Cited by 66
Metadata
- Authors
- Heukeshoven, J., & Dernick, R.
- Venue
- ELECTROPHORESIS
- Publisher
- Wiley
- Published
- 1988-01
- Volume
- 9
- Issue
- 1
- Pages
- 28-32
- ISSN
- 0173-0835 (ISSN-L)
- 1522-2683
- OpenAlex
- W1974956422
- Cited by
- 693
- References
- 0
- FWCI
- 12.17
- Type
- journal article
- Language
- en
- Open access
- closed
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- Heukeshoven, J., & Dernick, R. (1988). Improved silver staining procedure for fast staining in PhastSystem Development Unit. I. Staining of sodium dodecyl sulfate gels. ELECTROPHORESIS. https://doi.org/10.1002/elps.1150090106
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
