The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Routine identification of proteins from sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) gels or polyvinyl difluoride membranes using matrix assisted laser desorption/ionization‐time of flight‐mass spectrometry (MALDI‐TOF‐MS)
Abstract
As the resource laboratory for Rockefeller University our emphasis continues to be on methodology development for the routine analysis of low abundance proteins isolated from native sources. In the past ten years, gel electrophoresis of proteins has become the method of choice for the preparation of microgram and submicrogram quantities of protein for primary structural characterization, and over 95% of the samples submitted for protein identification are either in a gel or bound to polyvinyl difluoride membranes (PVDF). As such, we employ multiple microanalytical approaches encompassing Edman sequence degradation, amino acid and matrix assisted laser desorption/ionization‐time of flight (MALDI‐TOF) mass spectrometric analysis to provide an intergrated protein characterization of such samples. Here we describe the two major services we employ when providing protein identification from in‐gel or PVDF‐bound proteins.
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Citations
Cites
Surfactant effects on protein structure examined by electrospray ionization mass spectrometry
Loo, R. R. O., Dales, N., & Andrews, P. C. (1994) · Protein Science · doi:10.1002/pro.5560031109 · Cited by 184
Cited by
Gharahdaghi, F., Weinberg, C. R., Meagher, D. A., Imai, B. S., & Mische, S. M. (1999) · Electrophoresis · doi:10.1002/(sici)1522-2683(19990301)20:3<601::aid-elps601>3.0.co;2-6 · Cited by 913
Self-citationSegal, E. D., Cha, J., Lo, J., Falkow, S., & Tompkins, L. S. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.25.14559 · Cited by 770
Antibodies to a cell surface histone-like protein protect against Histoplasma capsulatum
Nosanchuk, J. D., Steenbergen, J. N., Shi, L., Deepe, G. S., & Casadevall, A. (2003) · Journal of Clinical Investigation · doi:10.1172/jci19361 · Cited by 178
Park, H.-H., Lee, K.-Y., Kim, S., Lee, J. W., Choi, N.-Y., Lee, E.-H., Lee, Y. J., Lee, S.-H., & Koh, S.-H. (2014) · Neurobiology of Aging · doi:10.1016/j.neurobiolaging.2013.12.015 · Cited by 72
Park, H.-H., Yu, H.-J., Kim, S., Kim, G., Choi, N.-Y., Lee, E.-H., Lee, Y. J., Yoon, M.-Y., Lee, K.-Y., & Koh, S.-H. (2016) · NeuroToxicology · doi:10.1016/j.neuro.2016.05.022 · Cited by 49
Metadata
- Authors
- Fernandez, J., Gharahdaghi, F., & Mische, S. M.
- Venue
- ELECTROPHORESIS
- Publisher
- Wiley
- Published
- 1998-05
- Volume
- 19
- Issue
- 6
- Pages
- 1036-1045
- ISSN
- 0173-0835 (ISSN-L)
- 1522-2683
- OpenAlex
- W2089771325
- Cited by
- 180
- References
- 1
- FWCI
- 4.29
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Routine identification of proteins from sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) gels or polyvinyl difluoride membranes using matrix assisted laser desorption/ionization‐time of flight‐mass spectrometry (MALDI‐TOF‐MS). https://app.cochat.ai/papers/10.1002/elps.1150190619
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- Fernandez, J., Gharahdaghi, F., & Mische, S. M. (1998). Routine identification of proteins from sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) gels or polyvinyl difluoride membranes using matrix assisted laser desorption/ionization‐time of flight‐mass spectrometry (MALDI‐TOF‐MS). ELECTROPHORESIS. https://doi.org/10.1002/elps.1150190619
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