The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Quantifying Western blots: Pitfalls of densitometry
Abstract
Although Western blots are frequently quantified, densitometry is not documented and appears to be based merely on traditions and guesswork. Confirming previous experience, none of 100 randomly selected and systematically scanned most recent papers provided sufficient information on how Western blot results were translated into statistical values. The importance of such information, however, becomes evident from our correlations of plasma erythropoietin values of various mammals determined using RIA and Western blot densitometry. Different common densitometry procedures applied to the identical Western blot revealed p‐values of these correlations ranging from 0.000013 to 0.76 reflecting the necessity of a scientifically sound basis for densitometry of Western blots. At present, the current lack of any definitions in densitometry opens the door to uncontrollable acquisition of any desired p‐value. Here we provide data that define what should be considered, what avoided and what documented when quantifying Western blots.
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Certain types of mitophagy induced by BNIP3L/NIX are highly dependent on GABARAP and less dependent on LC3 proteins.
“Moreover, certain types of mitophagy induced by BNIP3L/NIX are highly dependent on GABARAP and less dependent on LC3 proteins [286,287].”
From Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1 (2021) · cites this paper as [286]
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Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Acevedo Arozena, A., Adachi, H., Adams, C. M., Adams, P. D., Adeli, K., et al. (2016) · Autophagy · doi:10.1080/15548627.2015.1100356 · Cited by 5,894
Guidelines for the use and interpretation of assays for monitoring autophagy
Klionsky, D. J., Abdalla, F. C., Abeliovich, H., Abraham, R. T., Acevedo-Arozena, A., Adeli, K., Agholme, L., Agnello, M., Agostinis, P., Aguirre-Ghiso, J. A., et al. (2012) · Autophagy · doi:10.4161/auto.19496 · Cited by 3,996
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1
Klionsky, D. J., Abdel-Aziz, A. K., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., Abeliovich, H., Abildgaard, M. H., Abudu, Y. P., Acevedo-Arozena, A., et al. (2021) · Autophagy · doi:10.1080/15548627.2020.1797280 · Cited by 2,897
TrkB/BDNF signaling pathway and its small molecular agonists in CNS injury
Wang, Y., Liang, J., Xu, B., Yang, J., Wu, Z., & Cheng, L. (2024) · Life Sciences · doi:10.1016/j.lfs.2023.122282 · Cited by 152
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Multiplex quantitative assays indicate a need for reevaluating reported small-molecule TrkB agonists
Boltaev, U., Meyer, Y., Tolibzoda, F., Jacques, T., Gassaway, M., Xu, Q., Wagner, F., Zhang, Y.-L., Palmer, M., Holson, E., et al. (2017) · Science Signaling · doi:10.1126/scisignal.aal1670 · Cited by 94
Stahnke, T., Kowtharapu, B. S., Stachs, O., Schmitz, K.-P., Wurm, J., Wree, A., Guthoff, R. F., & Hovakimyan, M. (2017) · PLOS ONE · doi:10.1371/journal.pone.0172592 · Cited by 87
Erythropoietin's inhibiting impact on hepcidin expression occurs indirectly
Gammella, E., Diaz, V., Recalcati, S., Buratti, P., Samaja, M., Dey, S., Noguchi, C. T., Gassmann, M., & Cairo, G. (2015) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00410.2014 · Cited by 59
McGinley, C., & Bishop, D. J. (2016) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00630.2016 · Cited by 51
Weber, P., Kramer, A., Dingler, C., & Radde, N. (2012) · Bioinformatics · doi:10.1093/bioinformatics/bts377 · Cited by 14
“3.1 A model for secretion control at the trans-Golgi network Protein secretion in mammalian cells is a highly regulated process, in which the regulation of the formation of transport vesicles at the trans-Golgi network plays a crucial role.”
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Metadata
- Authors
- Gassmann, M., Grenacher, B., Rohde, B., & Vogel, J.
- Venue
- ELECTROPHORESIS
- Publisher
- Wiley
- Published
- 2009-06
- Volume
- 30
- Issue
- 11
- Pages
- 1845-1855
- ISSN
- 0173-0835
- 1522-2683
- Cited by
- 365
- References
- 1
- Language
- en
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