Cited by 132Microbial Metabolic Engineering and BioproductionAlgal biology and biofuel productionAdvanced Scientific Research Methods

Cheese whey as a renewable substrate for microbial lipid and biomass production by Zygomycetes

Vamvakaki, Afroditi‐Nectaria, Kandarakis, Ioannis, Kaminarides, Stelios, Komaitis, Michael, Papanikolaou, Seraphim·2010·Engineering in Life Sciences

Abstract

Three Zygomycetes, Mortierella isabellina, Thamnidium elegans and Mucor sp., were tested for their ability of producing biomass and lipid‐containing γ‐linolenic acid (GLA) during their cultivation on cheese whey. M. isabellina consumed all of the available lactose and a significant amount of the available protein. On the contrary, the two other fungi seemed incapable of consuming lactose after protein exhaustion. In the second series of experiments, for M. isabellina a supplementary quantity of lactose was added into the medium in order to increase the C/N ratio and hence to increase the production of fat. In the case of T. elegans and Mucor sp., a supplementary quantity of ammonium sulfate was added in order to favor the consumption of lactose and the production of biomass. Indeed, enhancement of lipid production was observed for M. isabellina and biomass production for T. elegans and Mucor sp.. Fatty acid analysis of the microbial lipid showed a composition that presented non‐negligible changes in relation with the age of the culture and the C/N molar ratio of the medium. Further analysis of the fat showed that the quantity of neutral lipids was the more abundant. The fatty acid composition of neutral lipids resembled to that of total lipids. Phospholipids were the more unsaturated fraction for Mucor sp. and M. isabellina. GLA was synthesized in all trials but its concentration presented differences related with the utilized strains and the fermentation time. Growth of M. isabellina on lactose‐supplemented whey resulted in a maximum GLA production of 301 mg/L.

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Crossref · 10.1002/elsc.201000063

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Authors
Vamvakaki, A., Kandarakis, I., Kaminarides, S., Komaitis, M., & Papanikolaou, S.
Affiliations
Agricultural University of Athens (GR)
Venue
Engineering in Life Sciences
Publisher
Wiley
Published
2010-08
Volume
10
Issue
4
Pages
348-360
ISSN
  • 1618-0240 (ISSN-L)
  • 1618-2863
OpenAlex
W2082394375
Cited by
132
References
54
Citations by year
2026: 6 · 2025: 2 · 2024: 11 · 2023: 13 · 2022: 10 · 2021: 5 · 2020: 10 · 2019: 3 · 2018: 10 · 2017: 10 · 2016: 6 · 2015: 8 · 2014: 16 · 2013: 4 · 2012: 11
FWCI
2.73
Type
journal article
Language
en
Open access
closed
Locations
Engineering in Life Sciences (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Advanced Scientific Research Methods
Keywords
whey, Zygomycetes, Mortierella isabellina, Thamnidium elegans, Mucor, microbial lipid production, gamma-linolenic acid, biomass production, lactose consumption, C/N ratio, fatty acid composition, phospholipids, neutral lipids, renewable substrates

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