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Embryonic expression of an Nkx2‐5/Cre gene using ROSA26 reporter mice
Abstract
Summary: Nkx2‐5, one of the earliest cardiac‐specific markers in vertebrate embryos, was used as a genetic locus to knock in the Cre recombinase gene by homologous recombination. Offspring resulting from heterozygous Nkx2‐5/Cre mice mated to ROSA26 (R26R) reporter mice provided a model system for following Nkx2‐5 gene activity by β‐galactosidase (β‐gal) activity. β‐gal activity was initially observed in the early cardiac crescent, cardiomyocytes of the looping heart tube, and in the epithelium of the first pharyngeal arch. In later stage embryos (10.5–13.5 days postcoitum, dpc), β‐gal activity was observed in the stomach and spleen, the dorsum of the tongue, and in the condensing primordium of the tooth. The Nkx2‐5/Cre mouse model should provide a useful genetic resource to elucidate the role of loxP manipulated genetic targets in cardiogenesis and other developmental processes. genesis 31:176–180, 2001. © 2001 Wiley‐Liss, Inc.
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Chen, C. Y., & Schwartz, R. J. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.26.15628 · Cited by 287
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Metadata
- Authors
- Moses, K. A., DeMayo, F., Braun, R. M., Reecy, J. L., & Schwartz, R. J.
- Venue
- genesis
- Publisher
- Wiley
- Published
- 2001-12
- Volume
- 31
- Issue
- 4
- Pages
- 176-180
- ISSN
- 1526-954X (ISSN-L)
- 1526-968X
- OpenAlex
- W1520697307
- Cited by
- 288
- References
- 1
- FWCI
- 0.65
- Type
- journal article
- Language
- en
- Open access
- closed
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