The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
Efficient method to generate single‐copy transgenic mice by site‐specific integration in embryonic stem cells
Abstract
Transgenic and gene‐targeted mutant mice provide powerful tools for analysis of the cellular processes involved in early development and in the pathogenesis of many diseases. Here we describe a transgene integration strategy mediated by site‐specific recombination that allows establishment of multiple embryonic stem (ES) cell lines carrying tetracycline‐inducible genes targeted to a specific locus to assure predictable temporal and spatial expression in ES cells and mice. Using homologous recombination we inserted an frt homing site into which tetracycline‐inducible transgenes can be integrated efficiently in the presence of FLPe recombinase. This strategy and the vectors described here are generally applicable to any locus in ES cells and should allow for the rapid production of mice with transgenes efficiently targeted to a defined site. genesis 44:23–28, 2006. © 2006 Wiley‐Liss, Inc.
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Cites
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Self-citationChromosomal silencing and localization are mediated by different domains of Xist RNA
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Self-citationSIRT1 redistribution on chromatin promotes genomic stability but alters gene expression during aging
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Clonal dynamics of native haematopoiesis
Sun, J., Ramos, A., Chapman, B., Johnnidis, J. B., Le, L., Ho, Y.-J., Klein, A., Hofmann, O., & Camargo, F. D. (2014) · Nature · doi:10.1038/nature13824 · Cited by 821
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Metadata
- Authors
- Beard, C., Hochedlinger, K., Plath, K., Wutz, A., & Jaenisch, R.
- Venue
- genesis
- Publisher
- Wiley
- Published
- 2006-01
- Volume
- 44
- Issue
- 1
- Pages
- 23-28
- ISSN
- 1526-954X (ISSN-L)
- 1526-968X
- OpenAlex
- W2052790535
- Cited by
- 481
- References
- 16
- FWCI
- 5.48
- Type
- journal article
- Language
- en
- Open access
- closed
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