Artificial liver: A forthcoming attraction
Open accessAbstract
We have developed a novel hepatocyte loaded hollow fiber bioreactor as a potential bioartificial liver. Freshly harvested rat hepatocytes were entrapped in a three-dimensional gel matrix within hollow fibers in a perfused bioreactor. Gel entrapment allowed cells to be cultured at high density while maintaining tissuespecific function. Hepatocyte function was evaluated in 10 bioreactors, each containing approximately 5 x 10 7 cells. Oxygen consumption averaged 0.32 pmole/cell/hr, albumin appearance averaged 0.60 pg/cell/hr, and lidocaine clearance (a measure of the P-450 activity) averaged 0.74 pg/cell/hr. Function persisted for the 7 days of the study. Electron microscopy at 7 days showed the distinctive ultrastructure of viable, differentiated hepatocytes: bile canaliculi, intercellular junctions, peroxisomes, abundant mitochondria, and glycogen granules. Maintenance of tissue specific function and ultrastructure suggests that this bioreactor configuration has potential as a device to support patients in liver failure, as well as to study hepatocytes in vitro.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
A human weighing 70 kg would require approximately 84 billion hepatocytes to attain at least 30% liver function.
“For instance, a human weighing 70 kg would require approximately 84 billion hepatocytes to attain at least 30 % liver function [190,191].”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [190]
Several studies have suggested that transplanting approximately 20-30% of the normal liver mass can provide sufficient function for the recipient.
“In the context of liver transplantation, several studies have suggested that transplanting approximately 20–30 % of the normal liver mass can provide sufficient function for the recipient.”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [190]
Cited by
Sorted by citations
Badylak, S. F., Taylor, D., & Uygun, K. (2011) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-071910-124743 · Cited by 1,041 · see citing works
Use of Decellularized Porcine Liver for Engineering Humanized Liver Organ
Barakat, O., Abbasi, S., Rodriguez, G., Rios, J., Wood, R. P., Ozaki, C., Holley, L. S., & Gauthier, P. K. (2012) · Journal of Surgical Research · doi:10.1016/j.jss.2011.09.033 · Cited by 208 · see citing works
Hussein, K. H., Ahmadzada, B., Correa, J. C., Sultan, A., Wilken, S., Amiot, B., & Nyberg, S. L. (2024) · Bioactive Materials · doi:10.1016/j.bioactmat.2024.06.001 · Cited by 28 · see citing works
“For instance, a human weighing 70 kg would require approximately 84 billion hepatocytes to attain at least 30 % liver function [190,191].”
Background
- Authors
- Sussman, N. L., & Kelly, J. H.
- Affiliations
- Baylor College of Medicine (US)
- Venue
- Hepatology
- Published
- 1993-06
- Volume
- 17
- Issue
- 6
- Pages
- 1163-1164
- ISSN
- 0270-9139 (ISSN-L)
- OpenAlex
- W1970967836
- Cited by
- 46
- References
- 0
- Citations by year
- 2025: 1 · 2024: 1 · 2021: 2 · 2020: 2 · 2018: 3 · 2017: 2 · 2016: 2 · 2015: 2 · 2014: 1
- FWCI
- 1.81
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Hepatology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- bioartificial liver, hollow fiber bioreactor, hepatocyte function, liver failure support, albumin synthesis, oxygen consumption, hepatocyte ultrastructure, in vitro hepatocyte culture
- MeSH terms
- Artificial Organs, Humans, Liver, Microscopy, Electron, Oxygen Consumption
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
