A silver lining in cell line authentication: Short tandem repeat analysis of 1373 cases in China from 2010 to 2019
Open accessAbstract
Continuous cell lines are practical models that are widely used in the study of disease mechanisms and particularly cancers. However, the issue of cell line cross‐contamination has existed since the 1960s, despite repeated advocation for cell line authentication by many experts. Furthermore, cell line abuse has been underestimated and underreported. The China Center for Type Culture Collection (CCTCC) received 1373 cell samples for authentication from 2010 to 2019, and has found that the quality of cell lines has improved during this time, offering a positive outlook for the future.
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The Chinese Center for Type Culture Collection reported a CCCM rate of 33.6% in samples that it authenticated over the period 2010-2019.
“The Chinese Center for Type Culture Collection (”
From The history, landscape, and outlook of human cell line authentication and security (2024) · cites this paper as [154]
The incidence of primary CCCM dropped from 85.5% to 54.1%; the highest authenticity rate was observed in corporate scientific settings (74.7%), followed by universities (62.2%) and hospitals (51.8%).
“The incidence of primary CCCM dropped from 85.5% to 54.1%; the highest authenticity rate was observed in corporate scientific settings (74.7%), followed by universities (62.2%) and hospitals (51.8%) [154].”
From The history, landscape, and outlook of human cell line authentication and security (2024) · cites this paper as [154]
Two non-verifiable identifiers, BGC-803 and BSG-823, were recognized misspellings of the contaminated cell lines BGC-823 or MGC-803.
“Two NV identifiers (BGC-803, BSG-823) were recognized misspellings (26) of the contaminated cell lines BGC-823 or MGC-803 (38–41)”
From Misspellings or “miscellings”—Non‐verifiable and unknown cell lines in cancer research publications (2024) · cites this paper as [41]
In 185/420 (44%) papers, all eight non-verifiable identifiers could represent misspellings of similarly named cell lines, which were typically HeLa-contaminated cancer cell lines.
“This revealed that in 185/420 (44%) papers, all 8 NV identifiers could represent misspellings of similarly named cell lines, which were typically HeLa-contaminated cancer cell lines (38–41).”
From Misspellings or “miscellings”—Non‐verifiable and unknown cell lines in cancer research publications (2024) · cites this paper as [41]
Three NV lines-BGC-803, GSE-1 and MGC-823-were reported as obtained from the China Center for Type Culture Collection.
“and 3 NV cell lines (BGC-803, GSE-1, MGC-823) were described as sourced from the China Center for Type Culture Collection (38–41).”
From Misspellings or “miscellings”—Non‐verifiable and unknown cell lines in cancer research publications (2024) · cites this paper as [41]
Studies that reported STR profiles for similarly named contaminated lines did not describe these NV cell lines.
“These NV cell lines were also not described by studies describing STR profiles for similarly named contaminated cell lines (38–41).”
From Misspellings or “miscellings”—Non‐verifiable and unknown cell lines in cancer research publications (2024) · cites this paper as [41]
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References 41 · Cited by 11
Research works printed but not linked: 2
- MacLeod RA, Dirks WG, Matsuo Y, Kaufmann M, Milch H, Drexler HG. Widespread intraspecies cross-contamination of human tumor cell lines arising at source. Int J Cancer. 1999;83:555-563.
- Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of Chinaed. Beijing: China Medical Science and Technology Press; 2020.
- Authors
- Gu, M., Yang, M., He, J., Xia, S., Zhang, Z., Wang, Y., Zheng, C., & Shen, C.
- Affiliations
- Wuhan University (CN)
- Corresponding author
- Shen, Chao
- Venue
- International Journal of Cancer
- Publisher
- Wiley
- Published
- 2021-09-14
- Volume
- 150
- Issue
- 3
- Pages
- 502-508
- ISSN
- 0020-7136 (ISSN-L)
- 1097-0215
- OpenAlex
- W3198899702
- Cited by
- 11
- References
- 41
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 2 · 2022: 3
- FWCI
- 0.63
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- International Journal of Cancer (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Identification and Quantification in Food
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Keywords
- cell line authentication, short tandem repeat profiling, cell line cross-contamination, immortalized cell lines, cancer cell lines
- MeSH terms
- Cell Line Authentication, China, Humans, Time Factors, Microsatellite Repeats, Cell Line, Tumor
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