Microheterogeneity of tubulin proteins in neuronal and glial cells from the mouse brain in culture.
Open accessAbstract
The microheterogeneity of the alpha and beta isoforms of tubulin in brain cells in culture was studied. The cells were prepared from two precise regions of the embryonic mouse brain (ED15), the striatum and the mesencephalon. It was possible to maintain virtually pure cultures of neuronal or glial cells up to 1 and 4 weeks in vitro, respectively. The tubulin heterogeneity of striatal and mesencephalic neurons was found to be very similar after a few days in culture. More precise examination of pure neurons from the striatum revealed that their tubulin content after 7 days in vitro exhibited the same degree of complexity as a control extract from a 4 day-old mouse brain. In fact, we could detect the presence of at least six alpha and nine beta tubulin isoforms. Among these isoforms a specific family of beta proteins (beta' tubulin) and the more acidic alpha proteins were present. Since these isoforms have, up to now, been found only in tubulin extracts prepared from the nervous system, our experiments suggest that they belong to the neuronal subpopulation of this tissue. This point is reinforced by their complete absence from the tubulin proteins extracted from pure glial cells even after several weeks in vitro. These results lead us to propose that brain tubulin microheterogeneity is associated with the presence of neurons and not of glia and may, therefore, play a specific role in maintaining neuronal shape and function.
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Different classes of microtubules appear to contain different isotypes of the tubulin subunits, and these different microtubules appear to be compartmentalized within neurons.
“Different classes of microtubules appear to contain different isotypes of the tubulin subunits (Gazes and Bamstable, 1982;Gazes and Sweadner, 1981;Moura Neto et al., 1983), and these different microtubules appear to be compartmentalized within neurons (Cumming et al., 1983).”
From Immunocytochemical localization of tubulin and microtubule-associated protein 2 during the development of hippocampal neurons in culture (1986) · cites this paper as Moura Neto et al., 1983
Moura Neto et al. is cited for the first clause, not the later claim about compartmentalization. The Results section ('Tubulin heterogeneity in virtually pure neuronal cells') and Discussion show multiple tubulin isoforms in neuronal cultures, but do not establish that distinct classes of microtubules contain different isotypes. The Introduction explicitly leaves open whether tubulin heterogeneity reflects different microtubules within individual cells or differences among cells; page numbers are unavailable in our copy. (LLM, conf=0.82)
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Research works printed but not linked: 2
- Weingarten,M.D., Lockwood,A.H., Hwo,S.Y. and Kirschner,M.W. (1975) Proc. Natl. Acad. Sci. USA, 72, 1858-1862.
- de Nchaud,B., Wolff,A., Jeantet,C. and Bourre,J.M. (1983) J. Neuro- chem., in press.
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Caceres, A., Banker, G., & Binder, L. (1986) · The Journal of Neuroscience · doi:10.1523/jneurosci.06-03-00714.1986 · Cited by 367 · see citing works
- Authors
- Moura Neto, V., Mallat, M., Jeantet, C., & Prochiantz, A.
- Affiliations
- Inserm (FR)
- Venue
- The EMBO Journal
- Published
- 1983-08-01
- Volume
- 2
- Issue
- 8
- Pages
- 1243-1248
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC555267
- OpenAlex
- W1642836149
- Cited by
- 81
- References
- 0
- Citations by year
- 2024: 1 · 2022: 1 · 2017: 1 · 2016: 1 · 2015: 2 · 2014: 4 · 2013: 2 · 2012: 1
- FWCI
- 3.01
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The EMBO Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
- Keywords
- tubulin isoforms, alpha-tubulin, beta-tubulin, striatum, midbrain, neurons, glial cells, primary neuronal cultures, embryonic mouse brain, neuronal identity
- MeSH terms
- Animals, Brain, Cells, Cultured, Neuroglia, Neurons, Tubulin, Protein Isoforms, Mice
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