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The human haptoglobin gene: transcriptional regulation during development and acute phase induction
Open accessAbstract
Haptoglobin is a plasma protein scarcely present in fetal but abundant in adult serum, where it is present at a concentration of approximately 150 mg/100 ml. In this paper we show by run-on experiments that the haptoglobin (Hp) gene is actively transcribed in adult but not in fetal liver nuclei. Studies with established cell lines indicate that the Hp gene is expressed in the hepatoma cells HepG2 but not in the hepatoma cell line Hep3B nor in HeLa cells. Plasmids carrying various segments of the 5' flanking region of the Hp gene fused to the chloramphenicol acetyl transferase (CAT) gene direct CAT transcription when introduced into HepG2 but are inactive in Hep3B and in HeLa cells, thus behaving like the resident chromosomal Hp gene. Deletion analysis defines a region, upstream to the transcription initiation site, essential for cell-specific expression. The Hp gene is induced in Hep3B cells by treatment with supernatant from LPS-stimulated monocytes (SMS), in a manner mimicking the acute phase reaction. We characterize the DNA segment necessary and sufficient for cell-specific expression of the Hp-CAT constructions in HepG2 and show that the same segment is also sufficient for acute phase induction in Hep3B.
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“Haptoglobin is a tetrachain (α2β2) glycoprotein that binds free hemoglobin following hemolysis as a means to prevent loss of iron through urinary excretion and subsequent kidney damage [7].”
From A battle for iron: host sequestration and Staphylococcus aureus acquisition (2012) · cites this paper as [7]
Citations
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Interleukin-6 and the acute phase response
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Molecular Mechanisms of Staphylococcus aureus Iron Acquisition
Hammer, N. D., & Skaar, E. P. (2011) · Annual Review of Microbiology · doi:10.1146/annurev-micro-090110-102851 · Cited by 379
“Haptoglobin is plentiful within the serum and becomes more abundant during inflammation (56).”
BackgroundCollagenase expression in the lungs of transgenic mice causes pulmonary emphysema
D'Armiento, J. M., Dalal, S. S., Okada, Y., Berg, R. A., & Chada, K. K. (1992) · Cell · doi:10.1016/0092-8674(92)90391-o · Cited by 376
Haptoglobin, inflammation and disease
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Metadata
- Authors
- Oliviero, S., Morrone, G., & Cortese, R.
- Affiliations
- European Molecular Biology Laboratory (DE)
- Venue
- The EMBO Journal
- Published
- 1987-07-01
- Volume
- 6
- Issue
- 7
- Pages
- 1905-1912
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC553575
- OpenAlex
- W408517226
- Cited by
- 123
- References
- 33
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 3 · 2019: 2 · 2018: 3 · 2017: 2 · 2015: 3 · 2014: 2 · 2012: 1
- FWCI
- 8.83
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- The EMBO Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Institutional Research Information System University of Turin (University of Turin) (submittedVersion)
- Use Siena air (University of Siena) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Hemoglobin structure and function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Immunology and Microbiology › Immunology › Cytokine Signaling Pathways and Interactions
- Keywords
- transcriptional regulation, acute phase response, fetal liver, hepatoma cell lines, HepG2 cells, Hep3B cells, 5'-flanking region, cell type-specific gene expression, chloramphenicol acetyltransferase reporter, developmental gene expression
- MeSH terms
- Adult, Base Sequence, Cell Line, Cloning, Molecular, Fetus, Gene Expression Regulation, Genes, Haptoglobins, HeLa Cells, Carcinoma, Hepatocellular, Humans, Liver, Liver Neoplasms, Molecular Sequence Data, Plasmids, Transcription, Genetic
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