The DOI matches its Crossref record; 43 of the 46 references it cites were found in a registry or an index.
Human immunodeficiency virus infection of CD4-bearing cells occurs by a pH-independent mechanism
Open accessAbstract
The effect of weak bases (NH4Cl and amantadine) and carboxylic ionophores (monensin) on the infection of CD4 (T4) positive human cell lines by HIV-1 is examined. These reagents, which raise the pH of acidic intracellular organelles, fail to inhibit HIV-1 entry and the events leading to viral protein synthesis at concentrations inhibitory for low pH-dependent fusogenic enveloped viruses. The infectivity of VSV (HIV-1) pseudotypes is unaffected by weak bases at concentrations causing 95% plaque reduction of VSV in its own envelope. HIV-1 dependent cell--cell fusion (syncytium formation) occurs in medium maintained at pH 7.4-7.6, and virions are not irreversibly inactivated by incubation in acid medium. Our results show that HIV-1 entry and membrane fusion do not require exposure to low pH. The production of infectious HIV-1 particles, however, is inhibited in cells treated with NH4Cl.
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Metadata
- Authors
- McClure, M. O., Marsh, M., & Weiss, R. A.
- Affiliations
- Institute of Cancer Research (GB)
- Venue
- The EMBO Journal
- Published
- 1988-02-01
- Volume
- 7
- Issue
- 2
- Pages
- 513-518
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC454348
- OpenAlex
- W108460990
- Cited by
- 295
- References
- 46
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 2 · 2023: 2 · 2022: 1 · 2021: 5 · 2019: 2 · 2018: 1 · 2017: 4 · 2016: 2 · 2015: 3 · 2014: 6 · 2013: 7 · 2012: 11
- FWCI
- 6.89
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The EMBO Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Funders
- Wellcome Trust
- Medical Research Council
- Topics
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and Retrovirus Studies
- Keywords
- HIV-1 infection, CD4+ T cells, membrane fusion, syncytium formation, ammonium chloride, amantadine, monensin, carboxylic ionophores, weak bases, viral protein synthesis
- MeSH terms
- Antigens, Differentiation, T-Lymphocyte, Cell Line, Cell Transformation, Viral, HIV, Humans, Hydrogen-Ion Concentration, Viral Envelope Proteins
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