The DOI matches its Crossref record; 43 of the 46 references it cites were found in a registry or an index.
Human immunodeficiency virus infection of CD4-bearing cells occurs by a pH-independent mechanism
Open accessAbstract
The effect of weak bases (NH4Cl and amantadine) and carboxylic ionophores (monensin) on the infection of CD4 (T4) positive human cell lines by HIV-1 is examined. These reagents, which raise the pH of acidic intracellular organelles, fail to inhibit HIV-1 entry and the events leading to viral protein synthesis at concentrations inhibitory for low pH-dependent fusogenic enveloped viruses. The infectivity of VSV (HIV-1) pseudotypes is unaffected by weak bases at concentrations causing 95% plaque reduction of VSV in its own envelope. HIV-1 dependent cell--cell fusion (syncytium formation) occurs in medium maintained at pH 7.4-7.6, and virions are not irreversibly inactivated by incubation in acid medium. Our results show that HIV-1 entry and membrane fusion do not require exposure to low pH. The production of infectious HIV-1 particles, however, is inhibited in cells treated with NH4Cl.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Barré-Sinoussi, F., Chermann, J. C., Rey, F., Nugeyre, M. T., Chamaret, S., Gruest, J., Dauguet, C., Axler-Blin, C., Vézinet-Brun, F., Rouzioux, C., et al. (1983) · Science · doi:10.1126/science.6189183 · Cited by 7,038
Gallo, R. C., Salahuddin, S. Z., Popovič, M., Shearer, G. M., Kaplan, M., Haynes, B. F., Palker, T. J., Redfield, R. R., Oleske, J. M., Safai, B., et al. (1984) · Science · doi:10.1126/science.6200936 · Cited by 3,639
The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus
Dalgleish, A. G., Beverley, P. C. L., Clapham, P. R., Crawford, D., Greaves, M. F., & Weiss, R. A. (1984) · Nature · doi:10.1038/312763a0 · Cited by 3,539
Self-citationAdachi, A., Gendelman, H. E., Koenig, S., Folks, T. M., Willey, R. L., Rabson, A. B., & Martín, M. Á. (1986) · Journal of Virology · doi:10.1128/jvi.59.2.284-291.1986 · Cited by 2,805
T-lymphocyte T4 molecule behaves as the receptor for human retrovirus LAV
Klatzmann, D., Champagne, É., Chamaret, S., Gruest, J., Guétard, D., Hercend, T., Gluckman, J. C., & Montagnier, L. (1984) · Nature · doi:10.1038/312767a0 · Cited by 2,435
Ohkuma, S., & Poole, B. (1978) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.75.7.3327 · Cited by 2,173
ACIDIFICATION OF THE ENDOCYTIC AND EXOCYTIC PATHWAYS
Mellman, I., Fuchs, R., & Helenius, A. (1986) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.55.070186.003311 · Cited by 2,045
The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain
Maddon, P. J., Dalgleish, A. G., McDougal, J. S., Clapham, P. R., Weiss, R. A., & Axel, R. (1986) · Cell · doi:10.1016/0092-8674(86)90590-8 · Cited by 2,003
Self-citationBinding of HTLV-III/LAV to T4 + T Cells by a Complex of the 110K Viral Protein and the T4 Molecule
McDougal, J. S., Kennedy, M. S., Sligh, J. M., Cort, S. P., Mawle, A. C., & Nicholson, J. K. A. (1986) · Science · doi:10.1126/science.3001934 · Cited by 989
Functional Regions of the Envelope Glycoprotein of Human Immunodeficiency Virus Type 1
Kowalski, M. M., Potz, J., Basiripour, L., Dorfman, T., Goh, W. C., Terwilliger, E. F., Dayton, A. I., Rosen, C. A., Haseltine, W. A., & Sodroski, J. (1987) · Science · doi:10.1126/science.3629244 · Cited by 848
Research works printed but not linked: 7
- Galloway,C.J., Dean,G.E., Marsh,M., Rudnick,G. and Mellman,I. (1983) Proc. Natl. Acad. Sci. USA, 80, 3334-3338.
- Haywood,A.M. (1988) In Wilschut,J. and Hoekstra,D. (eds), Cellular Membrane Fusion. Marcel Dekker, New York (in press).
- Marsh,M., Wellsteed,J., Kern,H., Harms,E. and Helenius,A. (1982) Proc. Natl. Acad. Sci. USA, 79, 5297-5301.
- Montagnier,L. et al. (1984) In Gallo,R.C., Essex,M. and Gross,L. (eds), Human T-Cell Leukemia -Lymphoma Viruses. Cold Spring Harbor Lab- oratory Press, Cold Spring Harbor, NY, pp.
- Ohkuma,S. and Poole,B. (1978) Proc. Natl. Acad. Sci. USA, 75, 3327- 3331.
- Poste,G. and Pasternak,C.A. (1978) In Poste,G. and Nicholson,G.C. (eds), Membrane Fusion. Elsevier/North Holland, Amsterdam, pp. 305 -367.
- Skehel,J., Bayley,P., Brown,E., Martin,S., Waterfield,M., White,J., Wilson,I. and Wiley,D. (1982) Proc. Natl. Acad. Sci. USA, 79, 968-972.
Cited by
Sorted by citations
Viral vector platforms within the gene therapy landscape
Bulcha, J. T., Wang, Y., Ma, H., Tai, P. W. L., & Gao, G. (2021) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-021-00487-6 · Cited by 1,404
Identification of the Envelope V3 Loop as the Primary Determinant of Cell Tropism in HIV-1
Hwang, S. S., Boyle, T. J., Lyerly, H. K., & Cullen, B. R. (1991) · Science · doi:10.1126/science.1905842 · Cited by 870
Pathogenesis of human immunodeficiency virus infection
Levy, J. A. (1993) · Microbiological Reviews · doi:10.1128/mr.57.1.183-289.1993 · Cited by 1,046
The Lipophilic Bullet Hits the Targets: Medicinal Chemistry of Adamantane Derivatives
Wanka, L., Iqbal, K., & Schreiner, P. R. (2013) · Chemical Reviews · doi:10.1021/cr100264t · Cited by 712
HIV Enters Cells via Endocytosis and Dynamin-Dependent Fusion with Endosomes
Miyauchi, K., Kim, Y., Latinovic, O., Va, M., & Melikyan, G. B. (2009) · Cell · doi:10.1016/j.cell.2009.02.046 · Cited by 682
Metadata
- Authors
- McClure, M. O., Marsh, M., & Weiss, R. A.
- Affiliations
- Institute of Cancer Research (GB)
- Venue
- The EMBO Journal
- Published
- 1988-02-01
- Volume
- 7
- Issue
- 2
- Pages
- 513-518
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC454348
- OpenAlex
- W108460990
- Cited by
- 295
- References
- 46
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 2 · 2023: 2 · 2022: 1 · 2021: 5 · 2019: 2 · 2018: 1 · 2017: 4 · 2016: 2 · 2015: 3 · 2014: 6 · 2013: 7 · 2012: 11
- FWCI
- 6.89
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The EMBO Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Funders
- Wellcome Trust
- Medical Research Council
- Topics
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and Retrovirus Studies
- Keywords
- HIV-1 infection, CD4+ T cells, membrane fusion, syncytium formation, ammonium chloride, amantadine, monensin, carboxylic ionophores, weak bases, viral protein synthesis
- MeSH terms
- Antigens, Differentiation, T-Lymphocyte, Cell Line, Cell Transformation, Viral, HIV, Humans, Hydrogen-Ion Concentration, Viral Envelope Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
