The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Xer-mediated site-specific recombination in vitro
Open accessAbstract
The Xer site-specific recombination system acts at ColE1 cer and pSC101 psi sites to ensure that these plasmids are in a monomeric state prior to cell division. We show that four proteins, ArgR, PepA, XerC and XerD are necessary and sufficient for recombination between directly repeated cer sites on a supercoiled plasmid in vitro. Only PepA, XerC and XerD are required for recombination at psi in vitro. Recombination at cer and psi in vitro requires negative supercoiling and is exclusively intramolecular. Strand exchange at cer produces Holliday junction-containing products in which only the top strands have been exchanged. This reaction requires the catalytic tyrosine residue of Xer C but not that of XerD. Recombination at psi gives catenated circular resolution products. Strand exchange at psi is sequential. XerC catalyses the first (top) strand exchange to make a Holiday junction intermediate and XerD catalyses the second (bottom) strand exchange.
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“The 11-kb LIR encoded a tyrosine recombinase that belonged to the integrase family.43 , 44”
From Whole genomic DNA sequencing and comparative genomic analysis of Arthrospira platensis: high genome plasticity and genetic diversity (2016) · cites this paper as [44]
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Citations
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Cited by
Sorted by citations
Items 101–136 of 136
mwr Xer site-specific recombination is hypersensitive to DNA supercoiling
Trigueros, S., Tran, T., Sorto, N. A., Newmark, J., Colloms, S. D., Sherratt, D. J., & Tolmasky, M. E. (2009) · Nucleic Acids Research · doi:10.1093/nar/gkp208 · Cited by 14
“XerC, XerD, PepA and ArgR, as well as in vitro recombination assays were purified as described previously (12).”
BackgroundMethodResultInfluentialSelf-citationColloms, S. D. (2004) · Handbook of Proteolytic Enzymes · doi:10.1016/b978-0-12-079611-3.50277-9 · Cited by 14
Self-citationKnittel, V., Sadana, P., Seekircher, S., Stolle, A.-S., Körner, B., Volk, M., Jeffries, C. M., Svergun, D. I., Heroven, A. K., Scrima, A., et al. (2020) · PLoS Pathogens · doi:10.1371/journal.ppat.1008552 · Cited by 13
“site-specific recombination through the synapsis and activation of the XerCD recombinase [51, 52].”
Hayes, F., Lubetzki, S. A., & Sherratt, D. J. (1997) · Gene · doi:10.1016/s0378-1119(97)00299-0 · Cited by 13
Self-citationThe role of FIS in the Rcd checkpoint and stable maintenance of plasmid ColE1
Blaby, I. K., & Summers, D. (2009) · Microbiology · doi:10.1099/mic.0.029777-0 · Cited by 12
“FIS binds to cer in a sequence-specific manner XerC, XerD, ArgR and PepA are both necessary and sufficient for cer-mediated dimer recombination in vitro (Colloms et al., 1996).”
BackgroundXer Site-specific Recombination
Grainge, I., & Sherratt, D. J. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.10.6763 · Cited by 12
Self-citationDNA Segregation in Enterobacteria
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Cloning and characterisation of the Proteus mirabilis xerD gene
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Plasmid Diversity and Adaptation Analyzed by Massive Sequencing ofEscherichia coliPlasmids
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The Xer/dif site-specific recombination system of Campylobacter jejuni
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Synthesizing supercoiled circular DNA molecules in vitro
Rezaei, S., Moncada-Restrepo, M., Leng, S., Chambers, J. W., & Leng, F. (2025) · Nucleic Acids Research · doi:10.1093/nar/gkaf889 · Cited by 6
“These methods mimic the natural mechanism by which E. coli utilizes the site-specific recombinase XerCD to convert plasmid dimers into monomers via the ∼ 280-bp cer sequence in ColE1 plasmids, as plasmids commonly form dimers through homologous recombination between sister plasmids [ 37–39 ].”
Overview of Dissemination Mechanisms of Genes Coding for Resistance to Antibiotics
Tolmasky, M. E. (2014) · ASM Press eBooks · doi:10.1128/9781555815615.ch16 · Cited by 6
Communication Between Accessory Factors and the Cre Recombinase at Hybrid psi-loxP sites
Akopian, A., Gourlay, S. C., James, H., & Colloms, S. D. (2006) · Journal of Molecular Biology · doi:10.1016/j.jmb.2006.01.050 · Cited by 5
Self-citationPepA and ArgR do not regulate Cre recombination at the bacteriophage P1 loxP site
Macdonald, A. I., Lu, Y., Kilbride, E. A., Akopian, A., & Colloms, S. D. (2008) · Plasmid · doi:10.1016/j.plasmid.2007.12.001 · Cited by 5
“This regulation ensures that recombination is exclusively intramolecular so that plasmid multimers are resolved to monomers but not vice versa (Alén et al., 1997; Colloms et al., 1996; Summers, 1989).”
BackgroundSelf-citationMidonet, C., & Barre, F. (2015) · ASM Press eBooks · doi:10.1128/9781555819217.ch7 · Cited by 5
Escherichia coliArgR mutants defective incer/Xer recombination, but not in DNA binding
Sénéchal, H., Delesques, J., & Szatmari, G. B. (2010) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2010.01921.x · Cited by 5
“These proteins are essential for recombination at cer in vivo and in vitro, but are not directly involved in the strand exchange reaction (Stirling et al., 1988a, b, 1989; Colloms et al., 1996).”
BackgroundZhou, Q., Meng, Y., Su, L., Zhao, S., Shi, H., & Huang, S. (2008) · PubMed · Cited by 5
Colloms, S. D. (2012) · Handbook of Proteolytic Enzymes · doi:10.1016/b978-0-12-382219-2.00334-3 · Cited by 3
Self-citationProtein Production inEscherichia coli
Altenbuchner, J., & Mattes, R. (2008) · Protein Science Encyclopedia · doi:10.1002/9783527610754.tr08 · Cited by 2
Engineering serine integrase-based synthetic gene circuits for cellular memory and counting
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“coli plasmids, such as ColEI and pMB1, possess the cer target site (32) and recombination also requires the host accessory proteins, PepA and ArgR (the arginine repressor) (33).”
BackgroundMassant, J., Peeters, E., Charlier, D., & Maes, D. (2005) · Acta Crystallographica Section F Structural Biology and Crystallization Communications · doi:10.1107/s1744309105039618 · Cited by 1
“Moreover, the Escherichia coli repressor is involved in the sitespecific DNA-recombination mechanism that resolves multimeric forms of the ColE1 plasmid into its monomeric constituents (Stirling et al., 1988; Colloms et al., 1996).”
BackgroundBacterial chromosome replication: does precatenation occur in vivo?
Tolmatcheva, A. O. (2019) · Enlighten: Theses (The University of Glasgow) · doi:10.5525/gla.thesis.40938 · Cited by 1
Popov, M. N. (2012) · Max Planck Institute for Plasma Physics
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Colloms, S. D. (2025) · Handbook of Proteolytic Enzymes · doi:10.1016/b978-0-443-28849-4.00300-3
Self-citationSystème de recombinaison Xer chez Staphylococcus aureus
Gustinelli, A. (2011) · doi:10.71781/27203
Change in topological linking number during Xer recombination at the plasmid pSC101 psi sitePreprint
Provan, J. I., Tolmatcheva, A. O., Sherratt, D. J., & Colloms, S. D. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.02.23.706985
Self-citationHerdan, S., Kohm, K., Warneke, R., Roth, F., Glaser, N., Villani, L., Rüping, N., Dreher, L., Görge, N., Hoang, T. D., et al. (2026) · Journal of Bacteriology · doi:10.1128/jb.00155-26
Mathematical and experimental approaches to the dimer catastrophe theory
Field, C. M. (2011) · OpenGrey (Institut de l'Information Scientifique et Technique) · doi:10.17863/cam.16328
“, 1994); this requires PepA (Colloms et al., 1996) and ArcA (Colloms et al.”
Metadata
- Authors
- Colloms, S. D., McCulloch, R., Grant, K., Neilson, L., & Sherratt, D. J.
- Affiliations
- University of Oxford (GB)
- Venue
- The EMBO Journal
- Published
- 1996-03-01
- Volume
- 15
- Issue
- 5
- Pages
- 1172-1181
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC450016
- OpenAlex
- W1538904365
- Cited by
- 136
- References
- 43
- Citations by year
- 2026: 2 · 2025: 3 · 2023: 2 · 2022: 2 · 2020: 4 · 2019: 1 · 2017: 2 · 2016: 1 · 2015: 3 · 2014: 5 · 2013: 3 · 2012: 5
- FWCI
- 8.01
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The EMBO Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (submittedVersion)
- Funders
- Wellcome Trust
- Nuffield Foundation
- Medical Research Council
- Biotechnology and Biological Sciences Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Keywords
- XerC, XerD, ArgR, PEPA, plasmid replication, negative supercoiling, Holliday junction, strand exchange, intramolecular recombination
- MeSH terms
- Base Sequence, Binding Sites, DNA Nucleotidyltransferases, DNA, Superhelical, Escherichia coli, Molecular Sequence Data, Nucleic Acid Conformation, Plasmids, Recombination, Genetic, Substrate Specificity, Integrases, Escherichia coli Proteins, Recombinases
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