CoChat
Sign Up
Cited by 40Fermentation and Sensory AnalysisFungal and yeast genetics researchBiofuel production and bioconversion

The DOI matches its Crossref record.

Rapid Assessment of Yeast Viability and Yeast Vitality During Alcoholic Fermentation

Open access
Bouix, Marielle, Leveau, Jean-Yves·2001·Journal of the Institute of Brewing
DOI 10.1002/j.2050-0416.2001.tb00093.x
Open this paper in CoChat — free accountOpen PDF

Abstract

A flow cytometric method based on the measurement of the efflux percentage of carboxyfluorescein after staining of yeast cells with carboxyfluorescein diacetate is reported. This efflux percentage was followed throughout alcoholic fermentations performed with various pH and temperature conditions. The results obtained show that the efflux percentage is a good indicator of the energetic state of yeast cells and varies through the fermentation in a similar manner to the specific ethanol production rate. These results suggest that this flow cytometric method can be applied to assess viability and to predict vitality of yeast cells in alcoholic fermentations.

Overview

Integrity signals

Registry

Crossref · 10.1002/j.2050-0416.2001.tb00093.x

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 24

  1. Breeuwer, 1994, Applied and Environmental Microbiology, 60, 1467, 10.1128/AEM.60.5.1467-1472.1994 · DOI
  2. Bunthof, 1999, Applied and Environmental Microbiology, 65, 3681, 10.1128/AEM.65.8.3681-3689.1999 · DOI
  3. Chilver, 1978, Journal of the American Society of Brewing Chemists, 36, 13, 10.1094/ASBCJ-36-0013 · DOI
  4. Dinsdale, 1995, Journal of The Institute of Brewing, 101, 453, 10.1002/j.2050-0416.1995.tb00883.x · DOI
  5. Donhauser, 1992, Brauwelt, 132, 1301
  6. Fernandarosa, 1996, Applied and Environmental Microbiology, 57, 830
  7. Gant, 1993, Journal of Medical Microbiology, 39, 147, 10.1099/00222615-39-2-147 · DOI
  8. Hutter, 1992, Brauwelt, 132, 252
  9. Jespersen, 1994, Journal of The Institute of Brewing, 100, 399, 10.1002/j.2050-0416.1994.tb00837.x · DOI
  10. Jepras, 1995, Applied and Environmental Microbiology, 61, 2696, 10.1128/AEM.61.7.2696-2701.1995 · DOI
  11. Kara , B. V. Daoud , I. Searle , B. European Bravery Convention, Proceedings of the 21st Congress, Madrid 1987 409
  12. Laplace-Builhe, 1993, Biologie Cellulaire, 78, 123, 10.1016/0248-4900(93)90122-U · DOI
  13. Lloyd, 1996, Journal of The Institute of Brewing, 102, 251, 10.1002/j.2050-0416.1996.tb00910.x · DOI
  14. Millar, 1982, Biotechnology Letters, 9, 601, 10.1007/BF00127792 · DOI
  15. Pascual, 1997, Biotechnology and Bioengineering, 32, 374, 10.1002/bit.260320317 · DOI
  16. Parkkinen, 1976, Journal of The Institute of Brewing, 82, 283, 10.1002/j.2050-0416.1976.tb03773.x · DOI
  17. Porro, 1994, Biotechicai Progress, 10, 193, 10.1021/bp00026a009 · DOI
  18. Pyle, 1995, Applied and Environmental Microbiology, 61, 2614, 10.1128/AEM.61.7.2614-2619.1995 · DOI
  19. Quain, 1981, Journal of the Institute of Brewing, 87, 108, 10.1002/j.2050-0416.1981.tb04038.x · DOI
  20. Ribereau-Gayon, 1998, Traité d'oenologie, tome 1
  21. Rotman, 1966, Proceedings of Natural Academy of Science USA, 55, 134, 10.1073/pnas.55.1.134 · DOI
  22. Takeo, 1995, Applied and Environmental Microbiology, 10, 3604
  23. Yu, 1994, Journal of Microbiological Methods, 20, 1, 10.1016/0167-7012(94)90058-2 · DOI
  24. Analysis Comittee Report on Measurement of yeast viability, 1970, Journal of The Institute of Brewing, 76, 442, 10.1002/j.2050-0416.1970.tb03325.x · DOI

Cited by

Sorted by citations

See all 40

Metadata

Authors
Bouix, M., & Leveau, J.-Y.
ORCID
Bouix, Marielle
Venue
Journal of the Institute of Brewing
Publisher
Chartered Institute of Brewers and Distillers
Published
2001
Volume
107
Issue
4
Pages
217-225
ISSN
0046-9750 (ISSN-L)
DOI
10.1002/j.2050-0416.2001.tb00093.x
OpenAlex
W2006529830
Cited by
40
References
0
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2022: 1 · 2020: 1 · 2019: 5 · 2017: 1 · 2016: 2 · 2015: 4 · 2014: 4 · 2013: 2 · 2012: 4
FWCI
2.43
Type
journal article
Language
en
Open access
Licence
doi.wiley.com/10.1002/tdm_license_1.1 (tdm, 5356 d)
Locations
Journal of the Institute of Brewing (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Fermentation and Sensory Analysis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
Keywords
yeast viability, yeast vitality, alcoholic fermentation, carboxyfluorescein diacetate, flow cytometry, ethanol productivity, pH effect, temperature effects

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Rapid Assessment of Yeast Viability and Yeast Vitality During Alcoholic Fermentation. https://app.cochat.ai/papers/10.1002/j.2050-0416.2001.tb00093.x
The paper
Bouix, M., & Leveau, J.-Y. (2001). Rapid Assessment of Yeast Viability and Yeast Vitality During Alcoholic Fermentation. Journal of the Institute of Brewing. https://doi.org/10.1002/j.2050-0416.2001.tb00093.x