The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Crosslinking of biological tissues using genipin and/or carbodiimide
Abstract
The study was to investigate the crosslinking characteristics, mechanical properties, and resistance against enzymatic degradation of biological tissues after fixation with genipin (a naturally occurring crosslinking agent) and/or carbodiimide. Fresh tissue was used as a control. It was found that both genipin and carbodiimide are effective crosslinking agents for tissue fixation and genipin crosslinking is comparatively slower than carbodiimide crosslinking. Additionally, tissue fixation in genipin and/or carbodiimide may produce distinct crosslinking structures. Carbodiimide may form intrahelical and interhelical crosslinks within or between tropocollagen molecules, whereas genipin may further introduce intermicrofibrillar crosslinks between adjacent collagen microfibrils. The stability (denaturation temperature and resistance against enzymatic degradation) of the fixed tissue is mainly determined by its intrahelical and interhelical crosslinks. In contrast, intermicrofibrillar crosslinks significantly affect the mechanical properties (tissue shrinkage during fixation, tensile strength, strain at break, and ruptured pattern) of the fixed tissue. Moreover, the degree of enzymatic degradation of the fixed tissue may be influenced by three factors: the availability, to the enzyme, of recognizable cleavage sites, the degree of crosslinking, and the extent of helical integrity of tropocollagen molecules in tissue. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 64A: 427–438, 2003
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A Method for the Quantitative Modification and Estimation of Carboxylic Acid Groups in Proteins
Hoare, D. G., & Koshland, D. E. (1967) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)95981-8 · Cited by 934
Microelectrode studies on the acid microenvironment beneath adherent macrophages and osteoclasts*1
SILVER, I. (1988) · Experimental Cell Research · doi:10.1016/0014-4827(88)90191-7 · Cited by 896
Zero-length crosslinking procedure with the use of active esters
Grabarek, Z., & Gergely, J. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90267-d · Cited by 827
Cross-linking of dermal sheep collagen using a water-soluble carbodiimide
Olde Damink, L. H. H., Dijkstra, P. J., van Luyn, M. J. A., van Wachem, P. B., Nieuwenhuis, P., & Feijen, J. (1996) · Biomaterials · doi:10.1016/0142-9612(96)81413-x · Cited by 697
Biological factors affecting long-term results of valvular heterografts
Carpentier, A., Lemaigre, G., Robert, L., Carpentier, S., Dubost, C., & Gerbode, F. (1969) · The Journal of Thoracic and Cardiovascular Surgery · doi:10.1016/s0022-5223(19)42561-0 · Cited by 546
Sung, H.-W., Huang, R.-N., Huang, L. L. H., & Tsai, C.-C. (1999) · Journal of Biomaterials Science, Polymer Edition · doi:10.1163/156856299x00289 · Cited by 506
Self-citationTOUYAMA, R., TAKEDA, Y., INOUE, K., KAWAMURA, I., YATSUZUKA, M., IKUMOTO, T., SHINGU, T., YOKOI, T., & INOUYE, H. (1994) · Chemical and Pharmaceutical Bulletin · doi:10.1248/cpb.42.668 · Cited by 221
Stability of a biological tissue fixed with a naturally occurring crosslinking agent (genipin)
Sung, H.-W., Liang, I.-L., Chen, C.-N., Huang, R.-N., & Liang, H.-F. (2001) · Journal of Biomedical Materials Research · doi:10.1002/1097-4636(20010615)55:4<538::aid-jbm1047>3.0.co;2-2 · Cited by 213
Self-citationBiomaterial-associated calcification: pathology, mechanisms, and strategies for prevention.
Schöen, F. J., Harasaki, H., Kim, K. M., Anderson, H. C., & Levy, R. J. (1988) · PubMed · Cited by 200
Successive epoxy and carbodiimide cross-linking of dermal sheep collagen
Zeeman, R., Dijkstra, P. J., van Wachem, P. B., van Luyn, M. J. A., Hendriks, M., Cahalan, P. T., & Feijen, J. (1999) · Biomaterials · doi:10.1016/s0142-9612(98)00242-7 · Cited by 193
Cited by
Corneal Collagen Crosslinking: A Systematic Review
Sorkin, N., & Varssano, D. (2014) · Ophthalmologica · doi:10.1159/000357979 · Cited by 1,918
Macrophage Phenotype as a Determinant of Biologic Scaffold Remodeling
Badylak, S. F., Valentin, J. E., Ravindra, A. K., McCabe, G. P., & Stewart-Akers, A. M. (2008) · Tissue Engineering Part A · doi:10.1089/ten.tea.2007.0264 · Cited by 729
Dentin biomodification: strategies, renewable resources and clinical applications
Bedran-Russo, A. K., Pauli, G. F., Chen, S.-N., McAlpine, J., Castellan, C. S., Phansalkar, R. S., Aguiar, T. R., Vidal, C. M. P., Napotilano, J. G., Nam, J.-W., et al. (2014) · Dental Materials · doi:10.1016/j.dental.2013.10.012 · Cited by 309
Delgado, L. M., Bayon, Y., Pandit, A., & Zeugolis, D. I. (2015) · Tissue Engineering Part B: Reviews · doi:10.1089/ten.teb.2014.0290 · Cited by 272
Crosslinking strategies for preparation of extracellular matrix-derived cardiovascular scaffolds
Ma, B., Wang, X., Wu, C., & Chang, J. (2014) · Regenerative Biomaterials · doi:10.1093/rb/rbu009 · Cited by 193
Metadata
- Authors
- Sung, H., Chang, W., Ma, C., & Lee, M.
- Venue
- Journal of Biomedical Materials Research Part A
- Publisher
- Wiley
- Published
- 2003-01-27
- Volume
- 64A
- Issue
- 3
- Pages
- 427-438
- ISSN
- 1549-3296 (ISSN-L)
- 1552-4965
- OpenAlex
- W2158729728
- Cited by
- 286
- References
- 25
- FWCI
- 2.12
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Crosslinking of biological tissues using genipin and/or carbodiimide. https://app.cochat.ai/papers/10.1002/jbm.a.10346
- The paper
- Sung, H., Chang, W., Ma, C., & Lee, M. (2003). Crosslinking of biological tissues using genipin and/or carbodiimide. Journal of Biomedical Materials Research Part A. https://doi.org/10.1002/jbm.a.10346
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
