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Cited by 90Hydrogels: synthesis, properties, applicationsCollagen: Extraction and CharacterizationTissue Engineering and Regenerative Medicine

Novel multiarm PEG-based hydrogels for tissue engineering

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Tan, Huaping, DeFail, Alicia J., Rubin, J. Peter, Chu, Constance R., Marra, Kacey G.·2010·Journal of Biomedical Materials Research Part A
DOI 10.1002/jbm.a.32438PMC PMC2811768
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Abstract

Injectable scaffolds are promising substrates for regenerative medicine applications. In this study, multiarm amino‐terminated poly(ethylene glycol) (PEG) hydrogels were crosslinked with genipin, a compound naturally derived from the gardenia fruit. Four‐ and eight‐arm amino‐terminated PEG hydrogels crosslinked with varying concentrations of genipin were characterized. Both surface and cross‐sectional structures of PEG‐based hydrogels were observed by scanning electron microscopy. In vitro gelation time, water uptake, swelling, and weight loss of PEG hydrogels in phosphate buffered saline at 37°C were studied. The results showed that the eight‐arm PEG demonstrated a much slower gelation time compared with the four‐arm PEG, which may be due to the differing structures of the multiarm PEG hydrogels, which in turn affects the ability of genipin to react with the amine groups. Human adipose‐derived stem cells were seeded onto the four‐ and eight‐arm PEG hydrogels in vitro to assess the biological performance and applicability of the gels as cell carriers. The four‐arm PEG hydrogel resulted in enhanced cell adhesion when compared with the eight‐arm PEG hydrogel. Overall, these characteristics provide a potential opportunity for multiarm PEG hydrogels as injectable scaffolds in a variety of tissue engineering applications. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010

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  • PEG can be engineered into hydrogels that form a soft, flexible, water-retentive barrier mimicking natural tissue environments and helping maintain tissue separation during critical healing phases.

    “Furthermore, PEG can be engineered into hydrogels, forming a soft, flexible, and water-retentive barrier that closely mimics natural tissue environments, helping to maintain tissue separation during critical healing phases [212].”

    From Biomaterials in Postoperative Adhesion Barriers and Uterine Tissue Engineering (2025) · cites this paper as [212]

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Authors
Tan, H., DeFail, A. J., Rubin, J. P., Chu, C. R., & Marra, K. G.
Affiliations
University of Pittsburgh (US)
Corresponding author
Marra, Kacey G.
ORCID
Venue
Journal of Biomedical Materials Research Part A
Publisher
Wiley
Published
2009-03-16
Volume
92A
Issue
3
Pages
979-987
ISSN
  • 1549-3296 (ISSN-L)
  • 1552-4965
DOI
10.1002/jbm.a.32438
PMC
PMC2811768
OpenAlex
W1990956934
Cited by
90
References
1
Citations by year
2026: 2 · 2025: 10 · 2024: 6 · 2023: 10 · 2022: 4 · 2021: 4 · 2020: 4 · 2019: 3 · 2018: 8 · 2017: 3 · 2016: 3 · 2015: 6 · 2014: 6 · 2013: 7 · 2012: 7
FWCI
2.24
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Journal of Biomedical Materials Research Part A (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • U.S. Public Health Service (R01051963)
  • National Cancer Institute (R01CA114246)
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR051963)
  • National Institute of Biomedical Imaging and Bioengineering (T32EB001026)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
  • Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords
genipin crosslinking, injectable scaffolds, gelation time, swelling behavior, human adipose-derived stem cells, cell adhesion, tissue engineering, cell carriers
MeSH terms
Cell Adhesion, Humans, Microscopy, Electron, Scanning, Polyethylene Glycols, Adipocytes, Hydrogels, Tissue Engineering

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