The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Keratin hydrogels support the sustained release of bioactive ciprofloxacin
Abstract
Keratins are naturally derived proteins that can be fabricated into several biomaterial forms including hydrogels. These materials are a potential polymeric system for several tissue engineering and regenerative medicine applications due to their ability to support cell attachment, proliferation, and migration. However, little is known regarding their ability to support sustained release of therapeutic agents. This report describes the use of keratin hydrogels for sustained release of the antibiotic ciprofloxacin, which may prove useful to traumatic injury applications that would benefit from materials promoting tissue regeneration while also preventing acute infection. Hydrogels were formed from keratins obtained by oxidative extraction and known as keratose. We found that keratose hydrogels released ∼60% of loaded ciprofloxacin over the first 10 days and that continued release was detectable over the course of 3 weeks. Released ciprofloxacin was bioactive, inhibiting growth of Staphylococcus aureus for 23 days in vitro and for 2 weeks in a mouse subcutaneous model. The rate of ciprofloxacin release was highly correlated with degradation of the keratin hydrogel and not consistent with simple diffusion. Further experiments indicated that ciprofloxacin binds to keratose through electrostatic interactions. These studies demonstrate the specific use of keratose hydrogels for the release of antibiotic and the potential for the more general use of this material in tissue engineering and regenerative medicine applications. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Citations
Cites
The pharmacological basis of therapeutics
Frank, A. L. (1981) · Environmental Research · doi:10.1016/0013-9351(81)90176-6 · Cited by 2,738
The human keratins: biology and pathology
Moll, R., Divo, M., & Langbein, L. (2008) · Histochemistry and Cell Biology · doi:10.1007/s00418-008-0435-6 · Cited by 1,465
Kim, K., Luu, Y. K., Chang, C., Fang, D., Hsiao, B. S., Chu, B., & Hadjiargyrou, M. (2004) · Journal of Controlled Release · doi:10.1016/j.jconrel.2004.04.009 · Cited by 770
New consensus nomenclature for mammalian keratins
Schweizer, J., Bowden, P. E., Coulombe, P. A., Langbein, L., Lane, E. B., Magin, T. M., Maltais, L. J., Omary, M. B., Parry, D. A. D., Rogers, M. A., et al. (2006) · The Journal of Cell Biology · doi:10.1083/jcb.200603161 · Cited by 740
A Review of Keratin-Based Biomaterials for Biomedical Applications
Rouse, J. G., & Dyke, M. E. V. (2010) · Materials · doi:10.3390/ma3020999 · Cited by 662
Self-citationAntibiotic-eluting medical devices for various applications
ZILBERMAN, M., & ELSNER, J. (2008) · Journal of Controlled Release · doi:10.1016/j.jconrel.2008.05.020 · Cited by 610
Biochemical features of microbial keratinases and their production and applications
Brandelli, A., Daroit, D. J., & Riffel, A. (2009) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-009-2398-5 · Cited by 450
Some properties of keratin biomaterials: Kerateines
Hill, P., Brantley, H., & Van Dyke, M. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2009.09.076 · Cited by 349
Self-citationHill, P. S., Garrett, J. P., Ma, J., Apel, P. J., Klorig, D. C., Smith, T. L., Koman, L. A., Atala, A., & Dyke, M. E. V. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2007.08.023 · Cited by 346
Self-citationLocal Antibiotic Therapy in the Treatment of Open Fractures and Osteomyelitis
Zalavras, C. G., Patzakis, M. J., & Holtom, P. D. (2004) · Clinical Orthopaedics and Related Research · doi:10.1097/01.blo.0000143571.18892.8d · Cited by 329
Research works printed but not linked: 1
- Gillespie JM, 1983, The structural proteins of hair: Isolation characterization and regulation of biosynthesis
Cited by
Sorted by citations
Keratin: dissolution, extraction and biomedical application
Shavandi, A., Silva, T. H., Bekhit, A. A., & Bekhit, A. E.-D. A. (2017) · Biomaterials Science · doi:10.1039/c7bm00411g · Cited by 552
Keratin - Based materials for biomedical applications
Feroz, S., Muhammad, N., Ratnayake, J. T., & Dias, G. J. (2020) · Bioactive Materials · doi:10.1016/j.bioactmat.2020.04.007 · Cited by 389
“The incorporation of Ciprofloxacin in a keratin hydrogel resulted in 60% of the drug release during 10 days [169].”
MethodRecent trends in protein and peptide-based biomaterials for advanced drug delivery
Varanko, A. K., Saha, S., & Chilkoti, A. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.08.008 · Cited by 375
“to locally deliver and sustain the release of ciprofloxacin, a broad-range antibiotic [114].”
Eslahi, N., Abdorahim, M., & Simchi, A. (2016) · Biomacromolecules · doi:10.1021/acs.biomac.6b01235 · Cited by 258
Borrowing From Nature: Biopolymers and Biocomposites as Smart Wound Care Materials
Suarato, G., Bertorelli, R., & Athanassiou, A. (2018) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2018.00137 · Cited by 199
Metadata
- Authors
- Saul, J. M., Ellenburg, M. D., de Guzman, R. C., & Dyke, M. V.
- Affiliations
- Virginia Tech - Wake Forest University School of Biomedical Engineering & Sciences (US)
- Wake Forest University Health Sciences (US)
- Corresponding author
- Saul, Justin M.
- ORCID
- Saul, Justin M.
- Venue
- Journal of Biomedical Materials Research Part A
- Publisher
- Wiley
- Published
- 2011-06-16
- Volume
- 98A
- Issue
- 4
- Pages
- 544-553
- ISSN
- 1549-3296 (ISSN-L)
- 1552-4965
- OpenAlex
- W2044217991
- Cited by
- 111
- References
- 45
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 1 · 2023: 3 · 2022: 7 · 2021: 7 · 2020: 14 · 2019: 6 · 2018: 13 · 2017: 14 · 2016: 13 · 2015: 7 · 2014: 10 · 2013: 4 · 2012: 4
- FWCI
- 2.78
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Biomedical Materials Research Part A (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
- Physical Sciences › Materials Science › Biomaterials › Silk-based biomaterials and applications
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Keywords
- keratin hydrogels, keratosis, ciprofloxacin, sustained drug release, antibiotic delivery, Staphylococcus aureus, antibacterial activity, hydrogel degradation, traumatic injury, infection control, tissue regeneration
- MeSH terms
- Animals, Anti-Infective Agents, Bacterial Infections, Biocompatible Materials, Ciprofloxacin, Delayed-Action Preparations, Drug Carriers, Humans, Keratins, Materials Testing, Mice, Inbred C57BL, Sheep, Hydrogels, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
