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Cited by 30Bone Tissue Engineering MaterialsHydrogels: synthesis, properties, applications3D Printing in Biomedical Research

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Mesenchymal stem cell growth on and mechanical properties of fibrin-based biomimetic bone scaffolds

Linsley, Chase S., Wu, Benjamin M., Tawil, Bill·2016·Journal of Biomedical Materials Research Part A
DOI 10.1002/jbm.a.35840
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Abstract

Using the microenvironment of healing bone tissue as inspiration, this study utilized fibrin hydrogels combined with collagen type I and calcium phosphate ceramics to create a biomimetic bone scaffold. The contribution each component had on the growth of mesenchymal stem cells (hMSC) was assessed, and changes in the scaffold's mechanical properties were measured by indentation testing. The results show cell growth was greatest in scaffolds with lower concentrations of fibrinogen complex and followed a similar trend with the addition of collagen. However, cell growth was greatest in fibrin scaffolds with high concentrations of fibrinogen complex when combined with hydroxyapatite-β-tricalcium phosphate. The fibrin scaffold's stiffness does not significantly change over time, but the addition of collagen to scaffolds with low concentrations of fibrinogen complex had significant increases in stiffness by day 14. These results demonstrate that hMSC do not rapidly degrade fibrin and fibrin-collagen scaffolds in vitro. The data reported here can aid in the design and fabrication of fibrin-based engineered tissues and cell delivery vehicles that promote hMSC growth and viability as well as meet the mechanical requirements of native tissues. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2945-2953, 2016.

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Metadata

Authors
Linsley, C. S., Wu, B. M., & Tawil, B.
Affiliations
University of California, Los Angeles (US)
Corresponding author
Tawil, Bill
ORCID
Venue
Journal of Biomedical Materials Research Part A
Publisher
Wiley
Published
2016-08-01
Volume
104
Issue
12
Pages
2945-2953
ISSN
1549-3296 (ISSN-L)
DOI
10.1002/jbm.a.35840
OpenAlex
W2480281633
Cited by
30
References
0
Citations by year
2024: 7 · 2023: 2 · 2022: 2 · 2021: 6 · 2020: 1 · 2019: 4 · 2018: 2 · 2017: 6
FWCI
1.93
Type
journal article
Language
en
Open access
closed
Licence
doi.wiley.com/10.1002/tdm_license_1.1 (tdm)
Locations
  • Journal of Biomedical Materials Research Part A (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Keywords
mesenchymal stem cells, fibrin gel, type I collagen, calcium phosphate ceramics, hydroxyapatite, beta-tricalcium phosphate, scaffold mechanical properties, scaffold stiffness, cell growth, tissue engineering, cell delivery vehicle, scaffold degradation
MeSH terms
Calcium Phosphates, Cell Line, Cell Survival, Fibrin, Humans, Durapatite, Biomimetics, Cell Proliferation, Tissue Scaffolds, Elastic Modulus, Mesenchymal Stem Cells

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