Photochemically crosslinked cell-laden methacrylated collagen hydrogels with high cell viability and functionality
Open accessAbstract
Irgacure 2959 (I2959) is widely used as a photoinitiator for photochemical crosslinking of hydrogels. However, the free radicals generated from I2959 have been reported to be highly cytotoxic. In this study, methacrylated collagen (CMA) hydrogels were photochemically crosslinked using two different photoinitiators (i.e., I2959 and VA086) and the effect of photoinitiator type, photoinitiator concentration (i.e., 0.02 and 0.1%) and crosslinking time (1 and 10 min) on gel morphology, compressive modulus, and stability were investigated. In addition, Saos‐2 cells were encapsulated within the hydrogels and the effect of photochemical crosslinking conditions on cell viability, metabolic activity, and osteoblast functionality was assessed. Scanning electron microscopy imaging showed that photochemical crosslinking decreased the porosity of the hydrogels resulting in decrease in water retention ability compared to uncrosslinked hydrogels. On the other hand, photochemical crosslinking improved the stability of CMA hydrogels ( p < 0.05). Uniaxial compression tests showed that increasing the photoinitiator concentration significantly improved the compressive modulus of CMA hydrogels ( p < 0.05). Results from the live–dead assay showed that VA086 crosslinked hydrogels exhibited higher cell viability compared to I2959 ( p < 0.05) crosslinked hydrogels indicating that VA086 is more cytocompatible compared to I2959. Furthermore, Alizarin Red S staining revealed a significantly more pronounced cell‐mediated mineralization on VA086 crosslinked hydrogels ( p < 0.05) indicating that Saos‐2 cells retain their normal functionality in the presence of VA086. In summary, these results indicate that VA086 is a more biocompatible photoinitiator compared to I2959 for the generation of photochemically crosslinked CMA hydrogels for tissue engineering applications. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2019.
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Methacrylation of collagen (ColMA) is an effective strategy to address collagen's drawbacks by introducing photocrosslinkable methacrylate groups, enabling rapid photopolymerization and improving structural stability while preserving the inherent bioactivity of collagen.
“Methacrylation of collagen (ColMA) has emerged as an effective strategy to address these drawbacks by introducing photocrosslinkable methacrylate groups, enabling rapid photopolymerization and improving structural stability while preserving the inherent bioactivity of collagen [12,13,14].”
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- Authors
- Nguyen, T., Watkins, K. E., & Kishore, V.
- Affiliations
- Florida Institute of Technology (US)
- Corresponding author
- Kishore, Vipuil
- Venue
- Journal of Biomedical Materials Research Part A
- Publisher
- Wiley
- Published
- 2019-04-07
- Volume
- 107
- Issue
- 7
- Pages
- 1541-1550
- ISSN
- 1549-3296 (ISSN-L)
- 1552-4965
- PMC
- PMC6527486
- OpenAlex
- W2921937046
- Cited by
- 83
- References
- 46
- Citations by year
- 2026: 9 · 2025: 18 · 2024: 14 · 2023: 11 · 2022: 12 · 2021: 9 · 2020: 6 · 2019: 4
- FWCI
- 2.67
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Biomedical Materials Research Part A (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Center for Scientific Review (1R15AR071102)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (1R15AR071102)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- photocrosslinking, photoinitiators, Irgacure 2959, Saos-2 cells, cell viability, compressive modulus, hydrogel porosity, hydrogel stability, cytocompatibility, tissue engineering scaffolds
- MeSH terms
- Calcification, Physiologic, Cell Survival, Collagen, Cross-Linking Reagents, Humans, Methacrylates, Photochemistry, Compressive Strength, Hydrogels, Cell Line, Tumor
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