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Cited by 157Bone Metabolism and DiseasesTGF-β signaling in diseasesProtease and Inhibitor Mechanisms

The DOI matches its Crossref record; 59 of the 61 references it cites were found in a registry or an index.

Osteoclast synthesis and secretion and activation of latent transforming growth factor β

Oursler, Merry Jo·1994·Journal of Bone and Mineral Research
DOI 10.1002/jbmr.5650090402
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Abstract

The coupling of bone resorption and formation suggests that autocrine and paracrine factors are produced and released within the local bone environment. Since osteoblasts secrete latent transforming growth factor β (TGF-β), and osteoclasts can activate this latent complex, one candidate paracrine coupling factor is TGF-β. To examine whether TGF-β is also a potential osteoclast-derived autocrine and paracrine factor, highly purified avian osteoclasts were examined for synthesis of TGF-β and the mechanism by which osteoclasts activate the latent TGF-β complex was investigated. TGF-β protein production was measured within 4 h of culture in osteoclast-conditioned media using growth factor inhibition of CCL-64 cells and verified by blocking effects with anti-TGF-β antibodies. Synthesis of TGF-β was confirmed by northern blotting and metabolic labeling. Northern blots of total RNA revealed that osteoclasts expressed the mRNA for TGF-β2, β3, and β4. Biosynthetic studies suggest that TGF-β2 was the principle form secreted in culture. Nearly all the TGF-β that was secreted had been activated by the osteoclasts. When presented with exogenous latent TGF-β, osteoclasts activated latent TGF-β from a variety of sources. Furthermore, osteoclast-conditioned media retained the ability to activate latent TGF-β during cell-free incubations at 37°C. Osteoclast-mediated activation was inhibited by proteinase inhibitors, weak base treatment of the cells, or disruption of the cytoskeletal network. These data suggest that osteoclasts may secrete proteinases into the extracellular milieu to activate latent TGF-β and that TGF-β may be an autocrine factor involved in regulating osteoclast activity.

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Crossref · 10.1002/jbmr.5650090402

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Citations

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Show all 61 references

Research works printed but not linked: 5

  1. Noda, 1988, TGF-β regulation of alkaline phosphatase (AP) gene expression in rat osteosarcoma (ROS 17/2.8) cells, Calcif Tissue Int, 42, A–34
  2. Pfeilschifter, 1988, Transforming growth factor beta (TGF-beta) inhibits bone resorption in fetal rat long bones, Calcif Tissue Int, 42, A–34
  3. Roberts, 1991, Ciba Foundation Symposium, 157, 7
  4. Chambers, 1991, Bone, Vol. 2, The Osteoclast, 2, 141
  5. Bonewald, 1990, Role of transforming growth factor-beta in bone remodeling, Clin Orthop, 250, 261, 10.1097/00003086-199001000-00036 · DOI

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Metadata

Authors
Oursler, M. J.
Affiliations
Mayo Clinic (US)
Corresponding author
Oursler, Merry Jo
Venue
Journal of Bone and Mineral Research
Publisher
Oxford University Press (OUP)
Published
1994-04-01
Volume
9
Issue
4
Pages
443-452
ISSN
  • 0884-0431 (ISSN-L)
  • 1523-4681
DOI
10.1002/jbmr.5650090402
OpenAlex
W2043164695
Cited by
153
References
61
Citations by year
2026: 1 · 2025: 1 · 2024: 5 · 2023: 1 · 2022: 4 · 2021: 4 · 2020: 1 · 2019: 6 · 2018: 4 · 2017: 6 · 2016: 1 · 2015: 3 · 2014: 4 · 2013: 6 · 2012: 5
FWCI
2.02
Type
journal article
Language
en
Open access
closed
Licence
academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
Locations
  • Journal of Bone and Mineral Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institutes of Health (AR4114-01A1)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › TGF-β signaling in diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
Keywords
osteoclasts, TGF-beta activation, TGF-beta secretion, autocrine regulation, paracrine signaling, bone resorption, bone formation, proteolytic enzymes, cytoskeleton, TGF-beta2, TGF-beta mRNA expression
MeSH terms
Animals, Bone Resorption, Cells, Cultured, Chickens, Osteoclasts, Osteogenesis, Protease Inhibitors, RNA, Messenger, Transforming Growth Factor beta

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