The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Development and characterization of a conditionally immortalized human fetal osteoblastic cell line
Abstract
We report the establishment of a human fetal osteoblast cell line derived from biopsies obtained from a spontaneous miscarriage. Primary cultures isolated from fetal tissue were transfected with a gene coding for a temperature-sensitive mutant (tsA58) of SV40 large T antigen along with a gene coding for neomycin (G418) resistance. Individual neomycin resistant colonies were screened for alkaline phosphatase (AP)-specific staining. The clone with the highest AP level, hFOB 1.19, was examined further for other osteoblast phenotypic markers. Incubation of hFOB cells at the permissive temperature (33.5°C) resulted in rapid cell division, whereas little or no cell division occurred at the restrictive temperature (39.5°C). Both AP activity and osteocalcin (OC) secretion increased in a dose-dependent manner following dihydroxyvitamin D3 (1,25-D3) treatment when cultured at either temperature. However, AP and 1,25-D3-induced OC levels were elevated in confluent hFOB cells cultured at 39.5°C compared with 33.5°C. Treatment of hFOB cells with 1–34 parathyroid hormone (PTH) resulted in an increase in cAMP levels. Upon reaching confluence, hFOB cultures went through programmed differentiation and formed mineralized nodules as observed by von Kossa staining. Further, immunostaining of postconfluent, differentiated hFOB cells showed that high levels of osteopontin, osteonectin, bone sialoprotein, and type I collagen were expressed. Therefore, the clonal cell line hFOB 1.19 provides a homogeneous, rapidly proliferating model system to study certain stages of human osteoblast differentiation.
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Cited by
Hofbauer, L. C., Gori, F., Riggs, B. L., Lacey, D. L., Dunstan, C. R., Spelsberg, T. C., & Khosla, S. (1999) · Endocrinology · doi:10.1210/endo.140.10.7034 · Cited by 794
Self-citationGanss, B., Kim, R. H., & Sodek, J. (1999) · Critical Reviews in Oral Biology & Medicine · doi:10.1177/10454411990100010401 · Cited by 489
miR-29 Modulates Wnt Signaling in Human Osteoblasts through a Positive Feedback Loop
Kapinas, K., Kessler, C., Ricks, T., Gronowicz, G., & Delany, A. M. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.116137 · Cited by 396
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Metadata
- Authors
- Dr. Harris, S. A., Enger, R. J., Riggs, L. B., & Spelsberg, T. C.
- Venue
- Journal of Bone and Mineral Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1995-02-01
- Volume
- 10
- Issue
- 2
- Pages
- 178-186
- ISSN
- 0884-0431 (ISSN-L)
- 1523-4681
- OpenAlex
- W2011311044
- Cited by
- 488
- References
- 19
- FWCI
- 4.50
- Type
- journal article
- Language
- en
- Open access
- closed
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- Dr. Harris, S. A., Enger, R. J., Riggs, L. B., & Spelsberg, T. C. (1995). Development and characterization of a conditionally immortalized human fetal osteoblastic cell line. Journal of Bone and Mineral Research. https://doi.org/10.1002/jbmr.5650100203
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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