A coding RNA segment that enhances the ribosomal recruitment of chicken ccn1 mRNA

Mukudai, Yoshiki; Kubota, Satoshi; Eguchi, Takanori; Sumiyoshi, Kumi; Janune, Danilo; Kondo, Seiji; Shintani, Satoru; Takigawa, Masaharu

doi:10.1002/jcb.22894

Cited by 5RNA and protein synthesis mechanismsConnective Tissue Growth Factor ResearchRNA Research and Splicing

A coding RNA segment that enhances the ribosomal recruitment of chicken ccn1 mRNA

Mukudai, Yoshiki, Kubota, Satoshi, Eguchi, Takanori, Sumiyoshi, Kumi, Janune, Danilo, Kondo, Seiji, Shintani, Satoru, Takigawa, Masaharu·2010·Journal of Cellular Biochemistry

DOI 10.1002/jcb.22894

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Abstract

CCN1, a member of the CCN family of proteins, plays important physiological or pathological roles in a variety of tissues. In the present study, we initially found a highly guanine–cytosine (GC)‐rich region of approximately 200 bp near the 5′‐end of the open reading frame, which was always truncated by amplification of the corresponding cDNA region through the conventional polymerase chain reaction. An RNA in vitro folding assay and selective ribonuclease digestion of the corresponding segment of the ccn1 mRNA confirmed the involvement of a stable secondary structure. Subsequent RNA electromobility‐shift assays demonstrated the specific binding of some cytoplasmic factor(s) in chicken embryo fibroblasts to the RNA segment. Moreover, the corresponding cDNA fragment strongly enhanced the expression of the reporter gene in cis at the 5′‐end, but did not do so at the 3′‐end. According to the results of a ribosomal assembly test, the effect of the mRNA segment can predominantly be ascribed to the enhancement of transport and/or entry of the mRNA into the ribosome. Finally, the minimal GC‐rich mRNA segment that was predicted and demonstrated to form a secondary structure was confirmed to be a functional regulatory element. Thus, we here uncover a novel dual‐functionality of the mRNA segment in the ccn1 open reading frame, which segment acts as a cis‐element that mediates posttranscriptional gene regulation, while retaining the information for the amino acid sequence of the resultant protein. J. Cell. Biochem. 111: 1607–1618, 2010. © 2010 Wiley‐Liss, Inc.

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Both the 5′-noncoding region of Ccn1 mRNA and a highly GC-rich region encoding part of the IGFBP domain appear to contain IRES.
“Both the 5′-non coding region of the Ccn1 mRNA and a highly GC-rich region encoding part of the IGFBP domain appear to contain IRES [50,51].”
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Authors: Mukudai, Y., Kubota, S., Eguchi, T., Sumiyoshi, K., Janune, D., Kondo, S., Shintani, S., & Takigawa, M.
Affiliations: SHOWA Medical University (JP)
Okayama University (JP)
Corresponding author: Kubota, Satoshi
Takigawa, Masaharu
ORCID: Mukudai, Yoshiki
Kubota, Satoshi
Eguchi, Takanori
Kondo, Seiji
Takigawa, Masaharu
Venue: Journal of Cellular Biochemistry
Publisher: Wiley
Published: 2010-11-29
Volume: 111
Issue: 6
Pages: 1607-1618
ISSN: 0730-2312 (ISSN-L)
1097-4644
DOI: 10.1002/jcb.22894
OpenAlex: W2143008747
Cited by: 5
References: 53
Citations by year: 2013: 2 · 2012: 1
FWCI: 0.65
Type: journal article
Language: en
Open access: closed
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: Journal of Cellular Biochemistry (publishedVersion)
PubMed (publishedVersion)
Funders: Japan Society for the Promotion of Science
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Connective Tissue Growth Factor Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Keywords: CYR61, GC-rich regions, mRNA secondary structure, cis-regulatory elements, post-transcriptional regulation, ribosome assembly, mRNA transport, chicken embryo fibroblasts, CCN family proteins
MeSH terms: Animals, Chick Embryo, Chickens, Ribosomes, RNA, RNA, Messenger, Blotting, Western, Reverse Transcriptase Polymerase Chain Reaction, Electrophoretic Mobility Shift Assay, Cysteine-Rich Protein 61

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A coding RNA segment that enhances the ribosomal recruitment of chicken ccn1 mRNA

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