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Multiplication‐stimulating activity for chicken embryo fibroblasts from rat liver cell conditioned medium: A family of small polypeptides
Abstract
A partially purified multiplication‐stimulating activity for chicken embryo fibroblasts in cell culture was isolated from rat liver cell conditioned medium (see preceding paper, Dulak and Temin, 1973). It has been analyzed by isoelectric focusing and by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. Multiplication‐stimulating activity resided in a family of at least four polypeptides which were similar in apparent molecular size, but different in electrical charge. These polypeptides have a specific activity of about 50,000 with respect to serum. One of them has been purified on a small scale to apparent homogeneity in a sodium dodecyl sulfate polyacrylamide gel.
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Citations
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Ignotz, R. A., & Massagué, J. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)35666-1 · Cited by 2,492
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Metadata
- Authors
- Dulak, N. C., & Temin, H. M.
- Venue
- Journal of Cellular Physiology
- Publisher
- Wiley
- Published
- 1973-04
- Volume
- 81
- Issue
- 2
- Pages
- 161-170
- ISSN
- 0021-9541 (ISSN-L)
- 1097-4652
- OpenAlex
- W2128590763
- Cited by
- 181
- References
- 0
- FWCI
- 16.31
- Type
- journal article
- Language
- en
- Open access
- closed
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- Dulak, N. C., & Temin, H. M. (1973). Multiplication‐stimulating activity for chicken embryo fibroblasts from rat liver cell conditioned medium: A family of small polypeptides. Journal of Cellular Physiology. https://doi.org/10.1002/jcp.1040810205
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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