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Cited by 67Mesenchymal stem cell researchBone Metabolism and DiseasesFibroblast Growth Factor Research

The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.

Autocrine fibroblast growth factor 18 mediates dexamethasone‐induced osteogenic differentiation of murine mesenchymal stem cells

Hamidouche, Zahia, Fromigué, Olivia, Nuber, Ulrike, Vaudin, Pascal, Pages, Jean‐Christophe, Ebert, Regina, Jakob, Franz, Miraoui, Hichem, Marie, Pierre J.·2010·Journal of Cellular Physiology
DOI 10.1002/jcp.22152
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Abstract

The potential of mesenchymal stem cells (MSC) to differentiate into functional bone forming cells provides an important tool for bone regeneration. The identification of factors capable of promoting osteoblast differentiation in MSCs is therefore critical to enhance the osteogenic potential of MSCs. Using microarray analysis combined with biochemical and molecular approach, we found that FGF18, a member of the FGF family, is upregulated during osteoblast differentiation induced by dexamethasone in murine MSCs. We showed that overexpression of FGF18 by lentiviral (LV) infection, or treatment of MSCs with recombinant human (rh)FGF18 increased the expression of the osteoblast specific transcription factor Runx2, and enhanced osteoblast phenotypic marker gene expression and in vitro osteogenesis. Molecular silencing using lentiviral shRNA demonstrated that downregulation of FGFR1 or FGFR2 abrogated osteoblast gene expression induced by either LV‐FGF18 or rhFGF18, indicating that FGF18 enhances osteoblast differentiation in MSCs via activation of FGFR1 or FGFR2 signaling. Biochemical and pharmacological analyses showed that the induction of phenotypic osteoblast markers by LV‐FGF18 is mediated by activation of ERK1/2‐MAPKs and PI3K signaling in MSCs. These results reveal that FGF18 is an essential autocrine positive regulator of the osteogenic differentiation program in murine MSCs and indicate that osteogenic differentiation induced by FGF18 in MSCs is triggered by FGFR1/FGFR2‐mediated ERK1/2‐MAPKs and PI3K signaling. J. Cell. Physiol. 224: 509–515, 2010. © 2010 Wiley‐Liss, Inc.

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Metadata

Authors
Hamidouche, Z., Fromigué, O., Nuber, U., Vaudin, P., Pages, J., Ebert, R., Jakob, F., Miraoui, H., & Marie, P. J.
Affiliations
  • Inserm (FR)
  • Lund University (SE)
  • Université de Tours (FR)
  • University of Würzburg (DE)
Corresponding author
Marie, Pierre J.
ORCID
Venue
Journal of Cellular Physiology
Publisher
Wiley
Published
2010-05-22
Volume
224
Issue
2
Pages
509-515
ISSN
  • 0021-9541 (ISSN-L)
  • 1097-4652
DOI
10.1002/jcp.22152
OpenAlex
W1964618776
Cited by
67
References
42
Citations by year
2026: 3 · 2025: 4 · 2024: 4 · 2023: 3 · 2022: 5 · 2021: 4 · 2020: 4 · 2019: 7 · 2018: 9 · 2017: 4 · 2016: 4 · 2015: 3 · 2014: 2 · 2013: 3 · 2012: 6
FWCI
1.56
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Journal of Cellular Physiology (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
  • Lund University Publications (Lund University) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fibroblast Growth Factor Research
Keywords
FGF18, mesenchymal stem cells, osteogenic differentiation, dexamethasone, autocrine signaling, FGFR1, FGFR2, ERK signaling, PI3K signaling, Runx2, bone regeneration
MeSH terms
Animals, Cell Differentiation, Cell Line, Dexamethasone, Fibroblast Growth Factors, Models, Biological, Osteogenesis, Signal Transduction, Up-Regulation, Gene Expression Regulation, Developmental, Phosphatidylinositol 3-Kinases, Autocrine Communication, Gene Silencing, Extracellular Signal-Regulated MAP Kinases, Mice, Receptor, Fibroblast Growth Factor, Type 1, Receptor, Fibroblast Growth Factor, Type 2, Mesenchymal Stem Cells

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