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Activation of a NADPH Oxidase From Horse Polymorphonuclear Leukocytes in a Cell- Free System
Abstract
A postnuclear cell fraction from resting horse polymorphonuclear (PMN) leukocytes incubated with fatty acid-salt ions such as oleate or linoleate generated a NADPH-dependent oxygen consumption and superoxide production. Oxidative activity was negligible or absent in the postnuclear fraction from mononuclear leukocytes, p-chloromercuribenzene sulfonic acid-treated granulocytes, and granulocytes from a patient with chronic granulomatous disease. Although consistently associated with the membrane fraction from resting PMN leukocytes, the superoxide-generating activity was shown to be dependent on a thus far unknown cytosolic constituent. The apparent Km's for NADPH and NADH (66 and 1,600 µM, respectively), the pH optimum for the reaction (7.0), the cyanide insensitivity, and transient nature of the reaction together with the stoichiometric relationship between oxygen uptake and NADPH oxidation led to the conclusion that in the presence of cytosol a cell-free latent respiratory burst oxidase can be converted into an active enzyme by interaction with oleate micelles.
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Citations
Cited by
The NOX Family of ROS-Generating NADPH Oxidases: Physiology and Pathophysiology
Bedard, K., & Krause, K.-H. (2007) · Physiological Reviews · doi:10.1152/physrev.00044.2005 · Cited by 6,989
Myeloperoxidase: friend and foe
Klebanoff, S. J. (2005) · Journal of Leukocyte Biology · doi:10.1189/jlb.1204697 · Cited by 2,282
NADPH Oxidases (NOX): An Overview from Discovery, Molecular Mechanisms to Physiology and Pathology
Vermot, A., Petit-Härtlein, I., Smith, S. M. E., & Fieschi, F. (2021) · Antioxidants · doi:10.3390/antiox10060890 · Cited by 766
The O2−-forming NADPH oxidase of the phagocytes: nature, mechanisms of activation and function
Rossi, F. (1986) · Biochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics · doi:10.1016/0304-4173(86)90005-4 · Cited by 658
The NADPH oxidase of professional phagocytes—prototype of the NOX electron transport chain systems
Cross, A. R., & Segal, A. W. (2004) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/j.bbabio.2004.03.008 · Cited by 489
Metadata
- Authors
- Heyneman, R. A., & Vercauteren, R. E.
- Venue
- Journal of Leukocyte Biology
- Publisher
- Oxford University Press (OUP)
- Published
- 1984-12-01
- Volume
- 36
- Issue
- 6
- Pages
- 751-759
- ISSN
- 0741-5400 (ISSN-L)
- 1938-3673
- OpenAlex
- W2181949230
- Cited by
- 228
- References
- 0
- FWCI
- 2.32
- Type
- journal article
- Language
- en
- Open access
- closed
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- Heyneman, R. A., & Vercauteren, R. E. (1984). Activation of a NADPH Oxidase From Horse Polymorphonuclear Leukocytes in a Cell- Free System. Journal of Leukocyte Biology. https://doi.org/10.1002/jlb.36.6.751
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
