Cited by 489Neutrophil, Myeloperoxidase and Oxidative MechanismsRedox biology and oxidative stressNitric Oxide and Endothelin Effects

The NADPH oxidase of professional phagocytes—prototype of the NOX electron transport chain systems

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Cross, Andrew R, Segal, Anthony W·2004·Biochimica et Biophysica Acta (BBA) - Bioenergetics

Abstract

The NADPH oxidase is an electron transport chain in "professional" phagocytic cells that transfers electrons from NADPH in the cytoplasm, across the wall of the phagocytic vacuole, to form superoxide. The electron transporting flavocytochrome b is activated by the integrated function of four cytoplasmic proteins. The antimicrobial function of this system involves pumping K+ into the vacuole through BKCa channels, the effect of which is to elevate the vacuolar pH and activate neutral proteases. A number of homologous systems have been discovered in plants and lower animals as well as in man. Their function remains to be established.

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Crossref · 10.1016/j.bbabio.2004.03.008

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Authors
Cross, A. R., & Segal, A. W.
Affiliations
  • Scripps Research Institute (US)
  • University College London (GB)
Corresponding author
Segal, Anthony W
Venue
Biochimica et Biophysica Acta (BBA) - Bioenergetics
Publisher
Elsevier BV
Published
2004-06
Volume
1657
Issue
1
Pages
1-22
ISSN
0005-2728 (ISSN-L)
OpenAlex
W2096901938
Cited by
489
References
335
Citations by year
2026: 7 · 2025: 19 · 2024: 15 · 2023: 20 · 2022: 6 · 2021: 16 · 2020: 19 · 2019: 14 · 2018: 17 · 2017: 15 · 2016: 27 · 2015: 27 · 2014: 19 · 2013: 24 · 2012: 29
FWCI
2.55
Type
journal article
Language
en
Open access
Locations
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • UCL Discovery (University College London) (submittedVersion)
Funders
  • Wellcome Trust (074816)
  • National Institutes of Health
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
Keywords
NADPH oxidase, phagocytes, superoxide production, electron transport chain, phagosome, BKCa channels, vacuolar pH, antimicrobial defense
MeSH terms
Animals, Enzyme Activation, Humans, Isoenzymes, Phagocytes, Respiratory Burst, NADPH Oxidases, Electron Transport Chain Complex Proteins

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