The NADPH oxidase of professional phagocytes—prototype of the NOX electron transport chain systems
Open accessAbstract
The NADPH oxidase is an electron transport chain in "professional" phagocytic cells that transfers electrons from NADPH in the cytoplasm, across the wall of the phagocytic vacuole, to form superoxide. The electron transporting flavocytochrome b is activated by the integrated function of four cytoplasmic proteins. The antimicrobial function of this system involves pumping K+ into the vacuole through BKCa channels, the effect of which is to elevate the vacuolar pH and activate neutral proteases. A number of homologous systems have been discovered in plants and lower animals as well as in man. Their function remains to be established.
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NOX2 is the main NOX isoform in neutrophils and macrophages where it mediates the oxidative burst.
“NOX2 is the main NOX isoform in neutrophils and macrophages where it mediates the oxidative burst (198, 845).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [198]
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- Authors
- Cross, A. R., & Segal, A. W.
- Affiliations
- Scripps Research Institute (US)
- University College London (GB)
- Corresponding author
- Segal, Anthony W
- ORCID
- Segal, Anthony W
- Venue
- Biochimica et Biophysica Acta (BBA) - Bioenergetics
- Publisher
- Elsevier BV
- Published
- 2004-06
- Volume
- 1657
- Issue
- 1
- Pages
- 1-22
- ISSN
- 0005-2728 (ISSN-L)
- PMC
- PMC2636547
- OpenAlex
- W2096901938
- Cited by
- 489
- References
- 335
- Citations by year
- 2026: 7 · 2025: 19 · 2024: 15 · 2023: 20 · 2022: 6 · 2021: 16 · 2020: 19 · 2019: 14 · 2018: 17 · 2017: 15 · 2016: 27 · 2015: 27 · 2014: 19 · 2013: 24 · 2012: 29
- FWCI
- 2.55
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Biochimica et Biophysica Acta (BBA) - Bioenergetics (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Funders
- Wellcome Trust (074816)
- National Institutes of Health
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Keywords
- NADPH oxidase, phagocytes, superoxide production, electron transport chain, phagosome, BKCa channels, vacuolar pH, antimicrobial defense
- MeSH terms
- Animals, Enzyme Activation, Humans, Isoenzymes, Phagocytes, Respiratory Burst, NADPH Oxidases, Electron Transport Chain Complex Proteins
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