Low-intensity pulsed ultrasound increases cellular uptake of superparamagnetic iron oxide nanomaterial: results from human osteosarcoma cell line U2OS
Open accessAbstract
PURPOSE: To determine whether low-intensity pulsed ultrasound (LIPUS) is able to facilitate the uptake of a superparamagnetic iron oxide (SPIO) nanomaterial by cells that do not express high endocytosis capacity. MATERIALS AND METHODS: The human osteosarcoma cell line U2OS and a silica-coated SPIO functionalized peripherally with amines groups (overall diameter 8 nm) were used in this study. Adherent U2OS cells were labeled with SPIO by incubating with culture media containing the SPIO at 4.5 microg[Fe]/mL. LIPUS with the same parameters as those used in clinical application to accelerate bone fracture healing (1.5 MHz, duty cycle 1:4, spatial-average temporal-average intensity 30 mW/cm(2)) was applied to the cells at the beginning of the labeling process for 0, 0.5, 1, or 3 hours. The total incubation time with SPIO was 12 hours. SPIO labeling efficiency was evaluated with Prussian blue staining and a blueness measurement method, and magnetic resonance imaging (MRI) of cell pellets via measuring areas of SPIO-induced signal void. RESULTS: Both Prussian blue staining and in vitro MRI demonstrated that LIPUS application increased the SPIO nanomaterial labeling efficiency for U2OS cells in an exposure-duration-dependent manner. CONCLUSION: This study is a "proof of concept" that LIPUS can facilitate the cellular take-up of SPIO nanomaterial.
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Ultrasound has been implicated as potential approach to promote cell entry owing to ultrasound-induced mild hyperthermia and cell membrane deformation.
“Additionally, ultrasound has also been implicated as a potential approach to promote cell entry owing to ultrasound-induced mild hyperthermia and cell membrane deformation [176, 177].”
From Non-viral delivery systems for CRISPR/Cas9-based genome editing: challenges and opportunities (2018) · cites this paper as [177]
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- Authors
- Wang, Y. J., Leung, K. C., Cheung, W., Wang, H., Shi, L., Wang, D., Qin, L., & Ahuja, A. T.
- Affiliations
- Chinese University of Hong Kong (HK)
- Venue
- Journal of Magnetic Resonance Imaging
- Publisher
- Wiley
- Published
- 2010-05-24
- Volume
- 31
- Issue
- 6
- Pages
- 1508-1513
- ISSN
- 1053-1807 (ISSN-L)
- 1522-2586
- OpenAlex
- W2004620328
- Cited by
- 29
- References
- 28
- Citations by year
- 2025: 1 · 2021: 2 · 2020: 5 · 2019: 1 · 2018: 1 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 3 · 2013: 3 · 2012: 2
- FWCI
- 1.70
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Magnetic Resonance Imaging (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Ultrasound and Hyperthermia Applications
- Physical Sciences › Engineering › Biomedical Engineering › Characterization and Applications of Magnetic Nanoparticles
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- low-intensity pulsed ultrasound, superparamagnetic iron oxide nanoparticles, cellular uptake, U2OS osteosarcoma cells, cell labeling, endocytosis, MRI contrast agents, fracture healing
- MeSH terms
- Contrast Media, Dose-Response Relationship, Drug, Endocytosis, Ferric Compounds, Ferrocyanides, Humans, Magnetic Resonance Imaging, Ultrasonography, Ultrasonics, Nanotechnology, Cell Line, Tumor, Nanostructures
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