The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Direct imaging of single cells and tissue at sub‐cellular spatial resolution using transmission geometry MALDI MS
Abstract
The need of cellular and sub‐cellular spatial resolution in laser desorption ionization (LDI)/matrix‐assisted LDI (MALDI) imaging mass spectrometry (IMS) necessitates micron and sub‐micron laser spot sizes at biologically relevant sensitivities, introducing significant challenges for MS technology. To this end, we have developed a transmission geometry vacuum ion source that allows the laser beam to irradiate the back side of the sample. This arrangement obviates the mechanical/ion optic complications in the source by completely separating the optical lens and ion optic structures. We have experimentally demonstrated the viability of transmission geometry MALDI MS for imaging biological tissues and cells with sub‐cellular spatial resolution. Furthermore, we demonstrate that in conjunction with new sample preparation protocols, the sensitivity of this instrument is sufficient to obtain molecular images at sub‐micron spatial resolution. Copyright © 2012 John Wiley & Sons, Ltd.
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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751
Molecular Imaging of Biological Samples: Localization of Peptides and Proteins Using MALDI-TOF MS
Caprioli, R. M., Farmer, T. B., & Gile, J. (1997) · Analytical Chemistry · doi:10.1021/ac970888i · Cited by 2,234
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Cited by
Norris, J. L., & Caprioli, R. M. (2013) · Chemical Reviews · doi:10.1021/cr3004295 · Cited by 696
Self-citationKompauer, M., Heiles, S., & Spengler, B. (2017) · Nature Methods · doi:10.1038/nmeth.4071 · Cited by 600
Transmission-mode MALDI-2 mass spectrometry imaging of cells and tissues at subcellular resolution
Niehaus, M., Soltwisch, J., Belov, M. E., & Dreisewerd, K. (2019) · Nature Methods · doi:10.1038/s41592-019-0536-2 · Cited by 429
Mass Spectrometry Imaging of Biomolecular Information
Spengler, B. (2015) · Analytical Chemistry · doi:10.1021/ac504543v · Cited by 290
MALDI imaging mass spectrometry: Spatial molecular analysis to enable a new age of discovery
Gessel, M. M., Norris, J. L., & Caprioli, R. M. (2014) · Journal of Proteomics · doi:10.1016/j.jprot.2014.03.021 · Cited by 276
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Metadata
- Authors
- Zavalin, A., Todd, E. M., Rawhouser, P. D., Yang, J., Norris, J. L., & Caprioli, R. M.
- Venue
- Journal of Mass Spectrometry
- Publisher
- Wiley
- Published
- 2012-11
- Volume
- 47
- Issue
- 11
- Pages
- 1473-1481
- ISSN
- 1076-5174 (ISSN-L)
- 1096-9888
- OpenAlex
- W2313300359
- Cited by
- 236
- References
- 2
- FWCI
- 8.91
- Type
- journal article
- Language
- en
- Open access
- closed
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