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Cited by 15Cervical Cancer and HPV ResearchReproductive tract infections researchGenital Health and Disease

Human Papillomavirus genotype concordance between Anyplex II HPV28 and Linear array HPV genotyping test in anogenital samples

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Coutlée, François, de Pokomandy, Alexandra, Burchell, Ann N., El‐Zein, Mariam, Mayrand, Marie‐Hélène, Rodrigues‐Coutlée, Sophie, Money, Deborah, Comète, Émilie +3 more·2022·Journal of Medical Virology
DOI 10.1002/jmv.27605PMC PMC9126003
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Abstract

Anyplex II HPV‐28 (HPV‐28) can detect individually 28 HPV genotypes. We assessed the agreement between linear array HPV genotyping (LA‐HPV) and HPV‐28 for detection of 27 HPV genotypes in 410 stored anogenital samples (75 anal samples, 335 physician‐collected cervical samples) collected over 5 years from 410 individuals (13 men, 397 women), including 202 HIV‐seropositive individuals. HPV DNA was detected in 393 (95.9%, 95% confidence interval [CI]: 93.4–97.4) and 382 (93.2%, 95% CI: 90.3–95.3) samples with HPV‐28 and LA‐HPV (p = 0.13), respectively, for a good agreement of 96.3% (κ = 0.65). Of the 10503 HPV typing results, 10195 (780 positive, 9577 negative) were concordant, for an agreement of 97.1% (95% CI: 96.7–97.4) and an excellent of κ = 0.82 (95% CI: 0.80–0.84). The mean type‐specific concordance for 27 genotypes was 97.0%, 95% CI: 95.8–98.5 (κ = 0.86 ± 0.07, 95% CI: 0.83–0.88). Excellent agreement was obtained individually for all high‐risk genotypes (κ = 0.81–0.97) and for most other genotypes except for types 42, 44, 54, 68, and 69. The mean number of types per sample in discordant samples detected with LA‐HPV (3.0, 95% CI: 2.7–3.4) was greater than in concordant samples (1.4, 95% CI: 1.3–1.5; p< 0.001). In conclusion, HPV‐28 compared favorably with LA‐HPV, but was more frequently positive for HPV42 and HPV68.

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Authors
Coutlée, F., de Pokomandy, A., Burchell, A. N., El‐Zein, M., Mayrand, M., Rodrigues‐Coutlée, S., Money, D., Comète, É., McClymont, E., Rouleau, D., & Franco, E. L.
Affiliations
  • Centre Hospitalier de l’Université de Montréal (CA)
  • Department of Family Medicine McGill University Montreal Quebec Canada
  • St. Michael's Hospital (CA)
  • McGill University (CA)
  • University of British Columbia (CA)
Corresponding author
Coutlée, François
ORCID
Venue
Journal of Medical Virology
Publisher
Wiley
Published
2022-01-28
Volume
94
Issue
6
Pages
2824-2832
ISSN
  • 0146-6615 (ISSN-L)
  • 1096-9071
DOI
10.1002/jmv.27605
PMC
PMC9126003
OpenAlex
W4205723176
Cited by
15
References
35
Citations by year
2026: 2 · 2025: 4 · 2024: 5 · 2023: 2 · 2022: 2
FWCI
2.35
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Journal of Medical Virology (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institute of Allergy and Infectious Diseases (R01AI073889)
Topics
  • Health Sciences › Medicine › Epidemiology › Cervical Cancer and HPV Research
  • Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
  • Health Sciences › Medicine › Surgery › Genital Health and Disease
Keywords
human papillomavirus, HPV genotyping, genotype concordance, anogenital HPV infection, cervical HPV, anal HPV, people living with HIV, high-risk HPV genotypes, multiplex PCR, test agreement
MeSH terms
Cervix Uteri, DNA, Viral, Female, Genotype, Humans, Male, Sensitivity and Specificity, Papillomaviridae, Papillomavirus Infections, Alphapapillomavirus

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