Cited by 192Osteoarthritis Treatment and MechanismsMesenchymal stem cell researchPeriodontal Regeneration and Treatments

Chemotaxis of human articular chondrocytes and mesenchymal stem cells

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Mishima, Yasunori, Lotz, Martin·2008·Journal of Orthopaedic Research

Abstract

Migration of chondrocytes and mesenchymal stem cells (MSCs) may be important in cartilage development, tissue response to injury, and in tissue engineering. This study analyzed growth factors and cytokines for their ability to induce migration of human articular chondrocytes and bone marrow‐derived mesenchymal stem cells in Boyden chamber assays.In human articular chondrocytes serum induced dose‐ and time‐dependent increases in cell migration. Among a series of growth factors and cytokines tested only PDGF induced a significant increase in cell migration. The PDGF isoforms AB and BB were more potent than AA. There was an aging‐related decline in the ability of chondrocytes to migrate in response to serum and PDGF. Human bone marrow MSC showed significant chemotaxis responses to several factors, including FBS, PDGF, VEGF, IGF‐1, IL‐8, BMP‐4, and BMP‐7. In summary, these results demonstrate that directed cell migration is inducible in human articular chondrocytes and MSC. PDGF is the most potent factor analyzed, and may be useful to promote tissue integration during cartilage repair or tissue engineering. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 26:1407–1412, 2008

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Crossref · 10.1002/jor.20668

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Authors
Mishima, Y., & Lotz, M.
Affiliations
Scripps Research Institute (US)
Corresponding author
Lotz, Martin
Venue
Journal of Orthopaedic Research
Publisher
Wiley
Published
2008-05-07
Volume
26
Issue
10
Pages
1407-1412
ISSN
  • 0736-0266 (ISSN-L)
  • 1554-527X
OpenAlex
W2091018106
Cited by
192
References
34
Citations by year
2026: 1 · 2025: 7 · 2024: 5 · 2023: 9 · 2022: 11 · 2021: 20 · 2020: 7 · 2019: 11 · 2018: 13 · 2017: 10 · 2016: 6 · 2015: 14 · 2014: 13 · 2013: 13 · 2012: 14
FWCI
8.78
Type
journal article
Language
en
Open access
Locations
  • Journal of Orthopaedic Research® (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Institute on Aging (P01AG007996, AG 07996)
Topics
  • Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
  • Health Sciences › Medicine › Urology › Periodontal Regeneration and Treatments
Keywords
chemotaxis, cell migration, articular chondrocytes, mesenchymal stem cells, bone marrow-derived stem cells, platelet-derived growth factor, growth factors, cytokines, aging, cartilage repair, tissue engineering, serum
MeSH terms
Adult, Aged, Aged, 80 and over, Aging, Blood Proteins, Cartilage, Articular, Chemotaxis, Dose-Response Relationship, Drug, Humans, Middle Aged, Platelet-Derived Growth Factor, Time Factors, Chondrocytes, Protein Isoforms, Intercellular Signaling Peptides and Proteins, Mesenchymal Stem Cells

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