The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Evaluation of genipin for stabilization of decellularized porcine cartilage
Open accessAbstract
We speculate that an acellular osteochondral xenograft may be a good alternative to allografts for repair of focal articular cartilage lesions. In order to make a xenograft resistant to enzymatic degradation and to prevent a chronic immune response it may be beneficial to stabilize it through crosslinking. The concept is analogous to treatment of porcine bioprosthetic heart valves with glutaraldehyde. The purpose of this study was to evaluate genipin, a natural crosslinking agent with low cytotoxicity, for stabilization of decellularized cartilage. Porcine articular cartilage discs were decellularized in SDS and nucleases and then crosslinked in genipin. The utility of genipin was determined from its effects on degree of crosslinking, mechanical properties, dimensional stability, enzymatic resistance, and in vitro biocompatibility. Degree of crosslinking, compressive moduli, and collagenase resistance varied over a wide range depending on genipin concentration. The equilibrium compressive modulus could be increased from approximately 50% to more than 120% that of native cartilage, and the time to complete degradation by collagenase could be extended from less than 12 h to more than 15 days. Radial shrinkage of approximately 4% was observed at a genipin concentration of 0.1% wt/vol, and cartilage coefficient of friction against glass increased in a concentration-dependent manner. Autologous chondrocytes displayed little difference in viability or their ability to attach and spread over the surface of genipin-fixed cartilage compared to non-crosslinked cartilage during 6 weeks of culture. These results indicate that genipin may be efficacious for stabilization of a decellularized porcine osteochondral xenograft. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:1949-1957, 2017.
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“Taken together, crosslinking is a double-edged sword, and accurate control of the degradation rate is required to achieve an equilibrium between graft stability and functional remodeling [190, 195].”
From Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine (2023) · cites this paper as [195]
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“To address these challenges, crosslinking has proved its efficacy in promoting mechanical stability and inhibiting the rapid degradation of decellularized tissues, providing sufficient time for angiogenesis by endothelial cells and new ECM synthesis [195, 196].”
From Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine (2023) · cites this paper as [195]
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“Genipin reduces the antigenicity of decellularized tissues by decreasing free amino groups with the same mechanism as GA to maintain structural integrity [195, 206].”
From Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine (2023) · cites this paper as [195]
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Citations
Cites
A simplified method for the analysis of hydroxyproline in biological tissues
Kesava Reddy, G., & Enwemeka, C. S. (1996) · Clinical Biochemistry · doi:10.1016/0009-9120(96)00003-6 · Cited by 1,296
Glutaraldehyde as a fixative in bioprostheses and drug delivery matrices
Jayakrishnan, A., & Jameela, S. R. (1996) · Biomaterials · doi:10.1016/0142-9612(96)82721-9 · Cited by 498
Focal Cartilage Defects in the Knee Impair Quality of Life as Much as Severe Osteoarthritis
Heir, S., Nerhus, T. K., Røtterud, J. H., Løken, S., Ekeland, A., Engebretsen, L., & Årøen, A. (2009) · The American Journal of Sports Medicine · doi:10.1177/0363546509352157 · Cited by 423
EXTRACELLULAR MATRIX BIOSCAFFOLDS FOR ORTHOPAEDIC APPLICATIONS: A COMPARATIVE HISTOLOGIC STUDY
VALENTIN, J. E., BADYLAK, J. S., MCCABE, G. P., & BADYLAK, S. F. (2006) · The Journal of Bone and Joint Surgery-American Volume · doi:10.2106/00004623-200612000-00015 · Cited by 411
Effects of crosslinking degree of an acellular biological tissue on its tissue regeneration pattern
Liang, H., Chang, Y., Hsu, C., Lee, M., & Sung, H. (2003) · Biomaterials · doi:10.1016/j.biomaterials.2003.09.109 · Cited by 227
Risk factors for progressive cartilage loss in the knee
Biswal, S., Hastie, T. J., Andriacchi, T. P., Bergman, G. A., Dillingham, M. F., & Lang, P. K. (2002) · Arthritis & Rheumatism · doi:10.1002/art.10573 · Cited by 222
Fresh Osteochondral Allograft Transplantation for the Knee: Current Concepts
Sherman, S. L., Garrity, J., Bauer, K., Cook, J. L., Stannard, J. P., & Bugbee, W. D. (2014) · Journal of the American Academy of Orthopaedic Surgeons · doi:10.5435/00124635-201402000-00007 · Cited by 216
Extraction techniques for the decellularization of tissue engineered articular cartilage constructs
Elder, B. D., Eleswarapu, S. V., & Athanasiou, K. A. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2009.03.050 · Cited by 214
Liang, H., Chang, W., Lin, K., & Sung, H. (2003) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.10476 · Cited by 211
Kheir, E., Stapleton, T. W., Shaw, D., Jin, Z. M., Fisher, J., & Ingham, E. (2011) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.33171 · Cited by 150
Research works printed but not linked: 1
- Vilsack T, 2014, United States summary and state data
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Armiento, A. R., Stoddart, M. J., Alini, M., & Eglin, D. (2018) · Acta Biomaterialia · doi:10.1016/j.actbio.2017.11.021 · Cited by 579
Kasravi, M., Ahmadi, A., Babajani, A., Mazloomnejad, R., Hatamnejad, M. R., Shariatzadeh, S., Bahrami, S., & Niknejad, H. (2023) · Biomaterials Research · doi:10.1186/s40824-023-00348-z · Cited by 316
Golebiowska, A. A., Intravaia, J. T., Sathe, V. M., Kumbar, S. G., & Nukavarapu, S. P. (2024) · Bioactive Materials · doi:10.1016/j.bioactmat.2023.09.017 · Cited by 236
Decellularized tissues as platforms for in vitro modeling of healthy and diseased tissues
McCrary, M. W., Bousalis, D., Mobini, S., Song, Y. H., & Schmidt, C. E. (2020) · Acta Biomaterialia · doi:10.1016/j.actbio.2020.05.031 · Cited by 99
Rowland, C. R., Glass, K. A., Ettyreddy, A. R., Gloss, C. C., Matthews, J. R. L., Huynh, N. P. T., & Guilak, F. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.04.049 · Cited by 92
Bakhtiar, H., Ashoori, A., Rajabi, S., Pezeshki‐Modaress, M., Ayati, A., Mousavi, M. R., Ellini, M. R., Kamali, A., Azarpazhooh, A., & Kishen, A. (2021) · International Endodontic Journal · doi:10.1111/iej.13675 · Cited by 50
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van Hengel, E. V. A., van der Laan, L. J. W., de Jonge, J., & Verstegen, M. M. A. (2025) · Bioengineering · doi:10.3390/bioengineering12020136 · Cited by 46
García‐García, Ó. D., Soury, M. E., González-Quevedo, D., Sánchez‐Porras, D., Chato‐Astrain, J., Campos, F., & Carriel, V. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22020674 · Cited by 35
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Li, L., Zhang, W., Huang, M., Li, J., Chen, J., Zhou, M., & He, J. (2018) · Journal of Biomaterials Science Polymer Edition · doi:10.1080/09205063.2018.1528519 · Cited by 33
Ge, Y., Guo, G., Liu, K., Yang, F., Yang, L., Wang, Y., & Zhang, X. (2022) · Composites Part B Engineering · doi:10.1016/j.compositesb.2022.109667 · Cited by 31
Gelatin capsule residue‐based films crosslinked with the natural agent genipin
Iahnke, A. O. E. S., Stoll, L., Bellé, A. S., Hertz, P. F., Rios, A. D. O., Rahier, H., & Flôres, S. H. (2019) · Packaging Technology and Science · doi:10.1002/pts.2481 · Cited by 28
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Elder, S. H., Clune, J., Walker, J., & Gloth, P. (2017) · Journal of Functional Biomaterials · doi:10.3390/jfb8040043 · Cited by 27
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Hu, X., Li, S., Peng, P., Wang, B., Liu, W., Dong, X., Yang, X., Karabaliev, M., Yu, Q., & Gao, C. (2023) · BMEMat · doi:10.1002/bmm2.12026 · Cited by 22
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Chen, J., Han, S., Huang, M., Li, J., Zhou, M., & He, J. (2020) · International Journal of Polymeric Materials · doi:10.1080/00914037.2020.1825084 · Cited by 19
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Self-citationJoukar, A., Creecy, A., Karnik, S. J., Noori-Dokht, H., Trippel, S. B., Wallace, J. M., & Wagner, D. R. (2023) · Journal of the mechanical behavior of biomedical materials/Journal of mechanical behavior of biomedical materials · doi:10.1016/j.jmbbm.2023.105827 · Cited by 17
Light-activated decellularized extracellular matrix-based bioinks for enhanced mechanical integrity
Kim, M., Kang, D., Han, H., & Jang, J. (2025) · Materials Today Bio · doi:10.1016/j.mtbio.2025.101859 · Cited by 24
Elder, S. H., Mosher, M., Jarquin, P., Smith, P., & Chironis, A. (2020) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.34747 · Cited by 12
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Du, A., Liu, D., Zhang, W., Wang, X., & Chen, S. (2022) · Journal of Biomaterials Applications · doi:10.1177/08853282221104287 · Cited by 7
Noori-Dokht, H., Joukar, A., Karnik, S. J., Williams, T., Trippel, S. B., & Wagner, D. R. (2022) · Cartilage · doi:10.1177/19476035221093064 · Cited by 5
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Kupratis, M. E., Gonzalez, U., Rahman, A., Burris, D. L., Corbin, E. A., & Price, C. (2024) · Journal of Biomechanical Engineering · doi:10.1115/1.4064663 · Cited by 3
Santos, S., Neu, C. P., Grady, J. J., & Pierce, D. M. (2022) · Osteoarthritis and Cartilage Open · doi:10.1016/j.ocarto.2022.100233 · Cited by 2
Nguyen, N. T., Vu, B. D., Huynh, T. D., Bui, T. Q., & Tran, H. L. B. (2025) · Artificial Organs · doi:10.1111/aor.15037 · Cited by 1
Flórez, A. M., Jiménez, R. A., Torres, M. A., Braga, M. E. M., de Sousa, H. C., & Fontanilla, M. R. (2025) · Cell and Tissue Research · doi:10.1007/s00441-025-04027-4 · Cited by 1
Fu, J., Zhu, Z., Xue, C., Wang, P., Han, X., Bai, D., Fan, Y., Sun, Y., & Wang, H. (2026) · Chemical Engineering Journal · doi:10.1016/j.cej.2026.180089 · Cited by 1
Jayed, H. M. (2018) · Purdue e-Pubs (Purdue University System) · doi:10.7912/c29w87
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Metadata
- Authors
- Elder, S., Pinheiro, A., Young, C., Smith, P., & Wright, E.
- Affiliations
- Mississippi State University (US)
- University of Akron (US)
- Corresponding author
- Elder, Steven
- Venue
- Journal of Orthopaedic Research
- Publisher
- Wiley
- Published
- 2017-03-24
- Volume
- 35
- Issue
- 9
- Pages
- 1949-1957
- ISSN
- 0736-0266 (ISSN-L)
- 1554-527X
- PMC
- PMC6784830
- OpenAlex
- W2553572787
- Cited by
- 34
- References
- 34
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 2 · 2023: 5 · 2022: 4 · 2021: 5 · 2020: 3 · 2019: 4 · 2018: 4 · 2017: 2
- FWCI
- 3.69
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- onlinelibrary.wiley.com/termsAndConditions#am (am, 239 d)
- onlinelibrary.wiley.com/termsAndConditions#vor (vor)
- doi.wiley.com/10.1002/tdm_license_1.1 (tdm)
- Locations
- Journal of Orthopaedic Research® (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R15AR066926)
- National Science Foundation (1359437)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
- Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
- Keywords
- genipin, decellularized cartilage, crosslinking, articular cartilage repair, porcine cartilage, collagenase resistance, compressive modulus, dimensional stability, friction coefficient, chondrocyte viability, biocompatibility, enzymatic degradation
- MeSH terms
- Animals, Cartilage, Articular, Cross-Linking Reagents, Materials Testing, Swine, Collagenases, Compressive Strength, Iridoids, Tissue Scaffolds
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