The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Biologic response of human anterior cruciate ligamentocytes on collagen-patches to platelet-rich plasma formulations with and without leucocytes
Open accessAbstract
Due to the poor self‐healing capacities of the anterior cruciate ligament, previous primary repair attempts have failed. To enhance biologic healing, platelet rich plasma and collagen scaffold have shown promise in animal models. Platelet rich plasma (PRP) is already used in several clinical applications although outcomes are quite debated. The purpose of this study was to examine the effects of different PRP formulations during 21 days: With leucocytes and pure PRP on human anterior cruciate ligament‐derived ligamentocytes grown on collagen patches in 3D cell cultures in vitro. Three experimental groups were formed: 2.5% leucocyte rich PRP, 2.5% pure PRP, 20% leucocyte rich PRP, a negative control, and a positive control. Cell proliferation, cell phenotype on mRNA transcript level, and extracellular matrix production (total collagen and glycosaminoglycan content) were evaluated. DNA content and metabolic cell activity increased significantly in all groups on day 21 compared to day 7, except in the negative control. No changes in extracellular matrix production were detected. Different catabolic genes were induced depending on the concentration of leucocyte rich PRP. PRP with and without leucocytes treated anterior cruciate ligamentocytes significantly increased cell proliferation but not extracellular matrix production. However, the specific activation of different catabolic genes was dependent on the relative content of leucocytes. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:2733–2739, 2017.
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“Independently of the presence of leucocytes, PRP was correlated with increased cell proliferation but not extracellular matrix production.”
From Molecular and Biologic Effects of Platelet-Rich Plasma (PRP) in Ligament and Tendon Healing and Regeneration: A Systematic Review (2023) · cites this paper as [37]
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Cites
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The Long-term Consequence of Anterior Cruciate Ligament and Meniscus Injuries: Osteoarthritis
Lohmander, L. S., Englund, P. M., Dahl, L. L., & Roos, E. M. (2007) · The American Journal of Sports Medicine · doi:10.1177/0363546507307396 · Cited by 2,344
Eppley, B. L., Woodell, J. E., & Higgins, J. (2004) · Plastic and Reconstructive Surgery · doi:10.1097/01.prs.0000138251.07040.51 · Cited by 1,091
Platelet-Rich Plasma Differs According to Preparation Method and Human Variability
Mazzocca, A. D., McCarthy, M. B. R., Chowaniec, D. M., Cote, M. P., Romeo, A. A., Bradley, J. P., Arciero, R. A., & Beitzel, K. (2012) · Journal of Bone and Joint Surgery · doi:10.2106/jbjs.k.00430 · Cited by 508
Scleraxis positively regulates the expression of tenomodulin, a differentiation marker of tenocytes
Shukunami, C., Takimoto, A., Oro, M., & Hiraki, Y. (2006) · Developmental Biology · doi:10.1016/j.ydbio.2006.06.036 · Cited by 427
Biomechanics of knee ligaments: injury, healing, and repair
Woo, S. L.-Y., Abramowitch, S. D., Kilger, R., & Liang, R. (2006) · Journal of Biomechanics · doi:10.1016/j.jbiomech.2004.10.025 · Cited by 424
Optimization of Leukocyte Concentration in Platelet-Rich Plasma for the Treatment of Tendinopathy
McCarrel, T. M., Minas, T., & Fortier, L. A. (2012) · Journal of Bone and Joint Surgery · doi:10.2106/jbjs.l.00019 · Cited by 358
Basic science of anterior cruciate ligament injury and repair
Kiapour, A. M., & Murray, M. M. (2014) · Bone & Joint Research · doi:10.1302/2046-3758.32.2000241 · Cited by 328
Rapid Isolation of Total RNA from Small Samples of Hypocellular, Dense Connective Tissues
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Scleraxis and NFATc Regulate the Expression of the Pro-α1(I) Collagen Gene in Tendon Fibroblasts
Leéjard, V., Brideau, G., Blais, F., Ampornaramveth, R. S., Wagner, G., Roehrl, M. H. A., Noda, M., Duprez, D., Houillier, P., & Rossert, J. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m610113200 · Cited by 239
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Chalidis, B., Givissis, P., Papadopoulos, P., & Pitsilos, C. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24032744 · Cited by 74
“[37] studied the effect of PRP with different concentrations of leucocytes on human ACL fibroblasts cultured for 21 days.”
Background
Metadata
- Authors
- Krismer, A. M., Cabra, R. S., May, R. D., Frauchiger, D. A., Kohl, S., Ahmad, S. S., & Gantenbein, B.
- Affiliations
- University of Bern (CH)
- Corresponding author
- Gantenbein, Benjamin
- Venue
- Journal of Orthopaedic Research
- Publisher
- Wiley
- Published
- 2017-05-30
- Volume
- 35
- Issue
- 12
- Pages
- 2733-2739
- ISSN
- 0736-0266 (ISSN-L)
- 1554-527X
- OpenAlex
- W2613260403
- Cited by
- 14
- References
- 21
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 1 · 2022: 3 · 2021: 4 · 2020: 1 · 2019: 2
- FWCI
- 1.15
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Orthopaedic Research® (publishedVersion)
- PubMed (publishedVersion)
- BORIS (University Library Bern) (submittedVersion)
- Funders
- Universität Zürich
- Topics
- Health Sciences › Dentistry › Periodontics › Periodontal Regeneration and Treatments
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Tendon Structure and Treatment
- Health Sciences › Medicine › Surgery › Knee injuries and reconstruction techniques
- Keywords
- anterior cruciate ligament, platelet-rich plasma, collagen scaffolds, 3D cell culture, cell proliferation, extracellular matrix synthesis, catabolic gene expression, ligament healing, ligament repair
- MeSH terms
- Anterior Cruciate Ligament Injuries, Adult, Cells, Cultured, Extracellular Matrix, Humans, Male, Leukocyte Transfusion, Cell Proliferation, Platelet-Rich Plasma, Young Adult
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
