Determination of vitexin and isovitexin in pigeonpea using ultrasonic extraction followed by LC‐MS
Abstract
A method based on ultrasonic extraction (USE) followed by LC-MS is presented for the determination of vitexin and isovitexin in pigeonpea extracts in this study. The influential parameters of the USE procedure were optimized, and the optimal conditions were as follows: extraction solvent, 60% ethanol solution; liquid/solid ratio 10:1 (mL/g), extraction power, 250 W; temperature, 40-50 degrees C; and three extraction cycles, each cycle 15 min. Validation of the USE method was performed in terms of repeatability and reproducibility. RSDs for extraction yields were lower than 5.85 and 8.09%, respectively. The LOD and LOQ of chromatographic determination were 0.96 and 3.2 ng/mL for vitexin and 0.84 and 2.8 ng/mL for isovitexin. The method was also successfully applied for the determination of vitexin and isovitexin in stems, leaves, and root extracts of pigeonpea. From all these results, we may conclude that the developed method is appropriate for the quality control of pigeonpea and other plant extract products developed from pigeonpea.
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In previous reports, flavonoid glycosides such as orientin and apigenin-6,8-di-C-α-L-arabinoside, and stilbenes longistylin C and cajaninstilbene acid were well separated in a water-methanol system.
“In previous reports, flavonoid glycosides such as orientin and apigenin-6,8-di-Ca -L-arabinoside, and stilbenes longistylin C and cajaninstilbene acid were well separated in water-methanol system [24,28].”
Cites
Kimbaris, A. C., Siatis, N. G., Daferera, D. J., Tarantilis, P. A., Pappas, C. S., & Polissiou, M. G. (2006) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2004.12.003 · Cited by 281 · see citing works
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Wei, Z., Qi, X., Li, T., Luo, M., Wang, W., Zu, Y., & Fu, Y. (2015) · Separation and Purification Technology · doi:10.1016/j.seppur.2015.05.015 · Cited by 274 · see citing works
Self-citation
- Authors
- Fu, Y., Zu, Y., Liu, W., Zhang, L., Tong, M., Efferth, T., Kong, Y., Hou, C., & Chen, L.
- Affiliations
- Northeast Forestry University (CN)
- German Cancer Research Center (DE)
- Venue
- Journal of Separation Science
- Publisher
- Wiley
- Published
- 2008-02
- Volume
- 31
- Issue
- 2
- Pages
- 268-275
- ISSN
- 1615-9306 (ISSN-L)
- 1615-9314
- OpenAlex
- W2100687389
- Cited by
- 40
- References
- 1
- Citations by year
- 2026: 1 · 2025: 5 · 2023: 1 · 2022: 4 · 2021: 4 · 2020: 2 · 2017: 2 · 2016: 4 · 2015: 1 · 2014: 1 · 2013: 2 · 2012: 3
- FWCI
- 9.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Separation Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Traditional Chinese Medicine Analysis
- Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Keywords
- vitexin, isovitexin, Cajanus cajan, ultrasound-assisted extraction, flavonoids, quality control, plant extracts, extraction optimization
- MeSH terms
- Chromatography, High Pressure Liquid, Reference Standards, Mass Spectrometry, Temperature, Ultrasonics, Cajanus, Apigenin
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