Separation of alkaloids from herbs using high-speed counter-current chromatography
Abstract
Alkaloids represent a most widespread group of bioactive natural products. Because of their alkalinity and structural diversity, the fractionation and purification of the alkaloids from herbs can often present a number of practical difficulties using the conventional chromatographic techniques. High-speed counter-current chromatography (HSCCC) is a liquid-liquid partition chromatography with a support-free liquid stationary phase, and is gaining more and more popularity as a viable separation technique for bioactive compounds from natural resources. In the present review, focus is placed on the separation of alkaloids by both conventional HSCCC and pH-zone-refining counter-current chromatography (CCC) techniques from herbs. The review presents the separation of over 120 different alkaloid compounds from more than 30 plant species by the conventional HSCCC and pH-zone-refining CCC. Based on the data from the literature, the proper solvent systems for the separation of alkaloids by the conventional HSCCC and pH-zone-refining CCC are also summarized.
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HSCCC eliminates irreversible adsorption of analytes on the solid phase in conventional column chromatography and offers full recovery of target compounds.
“High-speed counter-current chromatography (HSCCC), being a kind of liquid–liquid partition chromatography, eliminates the irreversible adsorption of analytes on the solid phase in conventional column chromatography and offers full recovery of target compounds.”
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Fang, L., Li, J., Zhou, J., Wang, X., & Guo, L. (2017) · Molecules · doi:10.3390/molecules22081310 · Cited by 20 · see citing works
Self-citation
- Authors
- Fang, L., Liu, Y., Yang, B., Wang, X., & Huang, L.
- Affiliations
- Shandong Academy of Sciences (CN)
- Venue
- Journal of Separation Science
- Publisher
- Wiley
- Published
- 2011-08-29
- Volume
- 34
- Issue
- 19
- Pages
- 2545-2558
- ISSN
- 1615-9306 (ISSN-L)
- 1615-9314
- OpenAlex
- W2154872263
- Cited by
- 85
- References
- 61
- Citations by year
- 2025: 2 · 2024: 3 · 2023: 2 · 2022: 4 · 2021: 2 · 2020: 4 · 2019: 7 · 2018: 6 · 2017: 10 · 2016: 4 · 2015: 9 · 2014: 15 · 2013: 11 · 2012: 5
- FWCI
- 9.97
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Separation Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Health Sciences › Medicine › Complementary and alternative medicine › Traditional Chinese Medicine Analysis
- Keywords
- alkaloids, herbs, high-speed countercurrent chromatography, liquid-liquid partition chromatography, solvent systems, alkaloid isolation, natural products, plant extracts
- MeSH terms
- Alkaloids, Countercurrent Distribution, Plant Extracts, Plants, Medicinal
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