Cited by 85Chromatography in Natural ProductsAnalytical Chemistry and ChromatographyTraditional Chinese Medicine Analysis

Separation of alkaloids from herbs using high-speed counter-current chromatography

Fang, Lei, Liu, Yuqin, Yang, Bin, Wang, Xiao, Huang, Luqi·2011·Journal of Separation Science

Abstract

Alkaloids represent a most widespread group of bioactive natural products. Because of their alkalinity and structural diversity, the fractionation and purification of the alkaloids from herbs can often present a number of practical difficulties using the conventional chromatographic techniques. High-speed counter-current chromatography (HSCCC) is a liquid-liquid partition chromatography with a support-free liquid stationary phase, and is gaining more and more popularity as a viable separation technique for bioactive compounds from natural resources. In the present review, focus is placed on the separation of alkaloids by both conventional HSCCC and pH-zone-refining counter-current chromatography (CCC) techniques from herbs. The review presents the separation of over 120 different alkaloid compounds from more than 30 plant species by the conventional HSCCC and pH-zone-refining CCC. Based on the data from the literature, the proper solvent systems for the separation of alkaloids by the conventional HSCCC and pH-zone-refining CCC are also summarized.

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Crossref · 10.1002/jssc.201000857

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Authors
Fang, L., Liu, Y., Yang, B., Wang, X., & Huang, L.
Affiliations
Shandong Academy of Sciences (CN)
Venue
Journal of Separation Science
Publisher
Wiley
Published
2011-08-29
Volume
34
Issue
19
Pages
2545-2558
ISSN
  • 1615-9306 (ISSN-L)
  • 1615-9314
OpenAlex
W2154872263
Cited by
85
References
61
Citations by year
2025: 2 · 2024: 3 · 2023: 2 · 2022: 4 · 2021: 2 · 2020: 4 · 2019: 7 · 2018: 6 · 2017: 10 · 2016: 4 · 2015: 9 · 2014: 15 · 2013: 11 · 2012: 5
FWCI
9.97
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Separation Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
  • Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
  • Health Sciences › Medicine › Complementary and alternative medicine › Traditional Chinese Medicine Analysis
Keywords
alkaloids, herbs, high-speed countercurrent chromatography, liquid-liquid partition chromatography, solvent systems, alkaloid isolation, natural products, plant extracts
MeSH terms
Alkaloids, Countercurrent Distribution, Plant Extracts, Plants, Medicinal

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