Combination of ultrasound‐assisted ethyl chloroformate derivatization and switchable solvent liquid‐phase microextraction for the sensitive determination of l‐methionine in human plasma by GC–MS
Abstract
A green and fast analytical method for the determination of l‐methionine in human plasma is presented in this study. Preconcentration of the analyte was carried out by switchable solvent liquid phase microextraction after ethyl chloroformate derivatization reaction. Instrumental detection of the analyte was performed by means of gas chromatography–mass spectrometry. N,N‐Dimethyl benzylamine was used in the synthesis of switchable solvent. Protonated N,N‐dimethyl benzylamine volume, volume/concentration of sodium hydroxide, and vortex period were meticulously fixed to their optimum values. Besides, ethyl chloroformate, pyridine, and ethanol volumes were optimized in order to get high derivatization yield. After the optimization studies, limit of detection and quantitation values were attained as 3.30 and 11.0 ng/g, respectively, by the developed switchable solvent liquid phase microextraction gas chromatography–mass spectrometry method that corresponding to 76.7‐folds enhancement in detection power of the gas chromatography–mass spectrometry system. Applicability and accuracy of the switchable solvent liquid phase microextraction–gas chromatography–mass spectrometry method were also checked by spiking experiments. Percent recovery results were ranged from 97.8 to 100.5% showing that human plasma samples could be analyzed for its l‐methionine level by the proposed method.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In SHS-based methods, the subsequent pH change after CO2 solubilization is normally performed with strong acids or strong bases.
“First of all, in spite of employing CO 2 for the solubilization step, the subsequent pH change is normally performed with strong acids [14][15][16] or strong bases [17,18].”
From Use of green alternative solvents in dispersive liquid‐liquid microextraction: A review (2022) · cites this paper as [18]
Cites
Regulation of cell function by methionine oxidation and reduction
Hoshi, T., & Heinemann, S. H. (2001) · The Journal of Physiology · doi:10.1111/j.1469-7793.2001.0001j.x · Cited by 384 · see citing works
A solvent having switchable hydrophilicity
Jessop, P. G., Phan, L., Carrier, A. J., Robinson, S., Dürr, C. J., & Harjani, J. R. (2010) · Green Chemistry · doi:10.1039/b926885e · Cited by 329 · see citing works
Tertiary amine solvents having switchable hydrophilicity
Jessop, P. G., Kozycz, L. M., Rahami, Z. G., Schoenmakers, D., Boyd, A. R., Wechsler, D., & Holland, A. M. (2011) · Green Chemistry · doi:10.1039/c0gc00806k · Cited by 260 · see citing works
Advances in amino acid analysis
Kaspar, H., Dettmer, K., Gronwald, W., & Oefner, P. J. (2008) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-008-2421-1 · Cited by 233 · see citing works
Fekkes, D. (1996) · Journal of Chromatography B Biomedical Sciences and Applications · doi:10.1016/0378-4347(96)00057-6 · Cited by 221 · see citing works
Urinary amino acid analysis: A comparison of iTRAQ®–LC–MS/MS, GC–MS, and amino acid analyzer
Kaspar, H., Dettmer, K., Chan, Q. M., Daniels, S. B., Nimkar, S. B., Daviglus, M. L., Stamler, J., Elliott, P., & Oefner, P. J. (2009) · Journal of Chromatography B · doi:10.1016/j.jchromb.2009.05.019 · Cited by 171 · see citing works
Bauchart‐Thevret, C., Stoll, B., Chacko, S. K., & Burrin, D. G. (2009) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.91021.2008 · Cited by 148 · see citing works
Use of switchable solvents in the microextraction context
Lasarte‐Aragonés, G., Lucena, R., Cárdenas, S., & Valcárcel, M. (2014) · Talanta · doi:10.1016/j.talanta.2014.08.031 · Cited by 136 · see citing works
Highly Sensitive GC/MS/MS Method for Quantitation of Amino and Nonamino Organic Acids
Kvitvang, H. F. N., Andreassen, T., Adam, T., Villas-Bôas, S. G., & Bruheim, P. (2011) · Analytical Chemistry · doi:10.1021/ac103245b · Cited by 136 · see citing works
Wassef, R., Haenold, R., Hansel, A., Brot, N., Heinemann, S. H., & Hoshi, T. (2007) · Journal of Neuroscience · doi:10.1523/jneurosci.0322-07.2007 · Cited by 123 · see citing works
Increased lipid peroxidation as a mechanism of methionine-induced atherosclerosis in rabbits
Toborek, M. J., Kopieczna-Grzebieniak, E., Dróżdż, M., & Wieczorek, M. (1995) · Atherosclerosis · doi:10.1016/0021-9150(94)05516-l · Cited by 113 · see citing works
Molnár-Perl, I. (2003) · Journal of Chromatography A · doi:10.1016/s0021-9673(02)01537-6 · Cited by 97 · see citing works
Memon, Z. M., Yılmaz, E., & Soylak, M. (2016) · Journal of Molecular Liquids · doi:10.1016/j.molliq.2016.12.098 · Cited by 85 · see citing works
Agüı́, L. (2004) · Talanta · doi:10.1016/j.talanta.2004.05.002 · Cited by 80 · see citing works
Ezoddin, M., Abdi, K., & Lamei, N. (2016) · Talanta · doi:10.1016/j.talanta.2016.03.018 · Cited by 77 · see citing works
Namera, A., Yashiki, M., Nishida, M., & Kojima, T. (2002) · Journal of Chromatography B · doi:10.1016/s1570-0232(02)00075-2 · Cited by 76 · see citing works
Hušek, P., & Šimek, P. (2006) · Current Pharmaceutical Analysis · doi:10.2174/157341206775474007 · Cited by 75 · see citing works
Houzé, P., Gamra, S., Madelaine, I., Bousquet, B., & Gourmel, B. (2001) · Journal of Clinical Laboratory Analysis · doi:10.1002/jcla.1018 · Cited by 72 · see citing works
Mandalakis, M., Apostolaki, M. D., & Stephanou, E. G. (2009) · Journal of Chromatography A · doi:10.1016/j.chroma.2009.11.021 · Cited by 63 · see citing works
Chen, W., Yang, X.-Y., Hegeman, A. D., Gray, W. M., & Cohen, J. D. (2010) · Journal of Chromatography B · doi:10.1016/j.jchromb.2010.06.027 · Cited by 60 · see citing works
Revelsky, I. A., Yashin, Y. S., Sobolevsky, T. G., Revelsky, A. I., Miller, B., & Oriedo, V. (2003) · European Journal of Mass Spectrometry · doi:10.1255/ejms.581 · Cited by 58 · see citing works
Fiamegos, Y. C., Nanos, C. G., & Stalikas, C. D. (2004) · Journal of Chromatography B · doi:10.1016/j.jchromb.2004.09.013 · Cited by 56 · see citing works
Khan, M., & Soylak, M. (2016) · RSC Advances · doi:10.1039/c5ra25384e · Cited by 56 · see citing works
Oguri, S., Yokoi, K.-J., & Motohase, Y. (1997) · Journal of Chromatography A · doi:10.1016/s0021-9673(97)00664-x · Cited by 55 · see citing works
Deng, C., Li, N., & Zhang, X. (2004) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.1660 · Cited by 55 · see citing works
Mudiam, M. K. R., Ch, R., Jain, R., Saxena, P. N., Chauhan, A., & Murthy, R. C. (2012) · Journal of Chromatography B · doi:10.1016/j.jchromb.2012.08.035 · Cited by 50 · see citing works
Rameshgar, J., Hasheminasab, K. S., Adlnasab, L., & Ahmar, H. (2017) · Journal of Separation Science · doi:10.1002/jssc.201700141 · Cited by 50 · see citing works
Wang, X., Gao, M. J., Zhang, Z., Gu, H., Liu, T., Yu, N., Wang, X., & Wang, H. (2018) · Food Analytical Methods · doi:10.1007/s12161-018-1187-0 · Cited by 47 · see citing works
Quantitation of amino acids and amines by chromatography - methods and protocols
Molnár-Perl, I. (2005) · Journal of chromatography library · doi:10.1016/s0301-4770(05)x8001-0 · Cited by 46 · see citing works
Chemical derivatization of amino acids for in situ analysis of Martian samples by gas chromatography
Rodier, C. G., Sternberg, R., Raulin, F., & Vidal‐Madjar, C. (2001) · Journal of Chromatography A · doi:10.1016/s0021-9673(01)00625-2 · Cited by 45 · see citing works
Salimi, A., & Roushani, M. (2006) · Electroanalysis · doi:10.1002/elan.200603639 · Cited by 45 · see citing works
Rapid method for the determination of amino acids in serum by capillary electrophoresis
Shen, Z., Sun, Z., Wu, L., Wu, K., Sun, S., & Huang, Z. (2002) · Journal of Chromatography A · doi:10.1016/s0021-9673(02)01251-7 · Cited by 44 · see citing works
Chormey, D. S., Bodur, S., Baskın, D., Fırat, M., & Bakırdere, S. (2018) · Journal of Separation Science · doi:10.1002/jssc.201800223 · Cited by 38 · see citing works
Self-citationLi, G., Wu, D., Xie, W., Sha, Y., Lin, H., & Liu, B. (2013) · Journal of Chromatography A · doi:10.1016/j.chroma.2013.03.076 · Cited by 37 · see citing works
Ask, K. S., Lid, M., Øiestad, E. L., Pedersen‐Bjergaard, S., & Gjelstad, A. (2019) · Journal of Chromatography A · doi:10.1016/j.chroma.2019.06.013 · Cited by 36 · see citing works
Naeemullah, N., Shah, F., Kazi, T. G., Afridi, H. I., Khan, A. R., Arain, S. S., Arain, M. S., & Panhwar, A. H. (2015) · Analytical Methods · doi:10.1039/c5ay02882e · Cited by 32 · see citing works
Ghidotti, M., Fabbri, D., & Torri, C. (2018) · Talanta · doi:10.1016/j.talanta.2018.11.032 · Cited by 31 · see citing works
Sobhi, H. R., Behbahani, M., Ghambarian, M., Salimi, M., & Esrafili, A. (2019) · Microchemical Journal · doi:10.1016/j.microc.2019.05.020 · Cited by 29 · see citing works
Grecco, C. F., de Souza, I. D., Acquaro, V. R., & Queiroz, M. E. C. (2018) · Journal of the Brazilian Chemical Society · doi:10.21577/0103-5053.20180151 · Cited by 20 · see citing works
Yamasaki-Yashiki, S., Tachibana, S., & Asano, Y. (2012) · Analytical Biochemistry · doi:10.1016/j.ab.2012.06.019 · Cited by 18 · see citing works
Cheng, C., & Wu, S.-C. (2000) · Journal of Chromatography A · doi:10.1016/s0021-9673(00)00756-1 · Cited by 17 · see citing works
Beitollahi, H., Mohadesi, A., GHORBANI, F., Karimi‐Maleh, H., Baghayeri, M., & Hosseinzadeh, R. (2013) · CHINESE JOURNAL OF CATALYSIS (CHINESE VERSION) · doi:10.1016/s1872-2067(12)60582-8 · Cited by 17 · see citing works
Atomic Absorption Determination of Some Amino Acids Containing a Sulphur Group
El-Brashy, A. M., & Al-Ghannam, S. M. (1996) · Analytical Letters · doi:10.1080/00032719608002275 · Cited by 13 · see citing works
Gas chromatography—chemical ionization mass spectrometry in amino acid analysis of pyoverdins
Dallakian, P., & Budzikiewicz, H. (1997) · Journal of Chromatography A · doi:10.1016/s0021-9673(97)00641-9 · Cited by 12 · see citing works
Ma, Z., Xu, A., Du, Y., Wang, J., Zhao, H., Diao, G., Zhu, X., & Guo, X. (2017) · Fluid Phase Equilibria · doi:10.1016/j.fluid.2017.12.005 · Cited by 8 · see citing works
Role of Methionine in Nocturnal Melatonin Peak in the Pineal Gland
Бондаренко, Л. А. (2004) · Bulletin of Experimental Biology and Medicine · doi:10.1023/b:bebm.0000038144.51076.07 · Cited by 4 · see citing works
Grecco, C. F., de Souza, I. D., Acquaro, V. R., & Queiroz, M. E. C. (2019) · Figshare · doi:10.6084/m9.figshare.7591589 · Cited by 1 · see citing works
Flagged first, then by citations
1 of the 48 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Use of green alternative solvents in dispersive liquid‐liquid microextraction: A review
Grau, J., Azorín, C., Benedé, J. L., Chisvert, A., & Salvador, A. (2022) · Journal of Separation Science · doi:10.1002/jssc.202100609 · Cited by 131 · see citing works
Switchable-hydrophilicity solvent liquid-liquid microextraction
Alshana, U., Hassan, M., Al-Nidawi, M., Yilmaz, E., & Soylak, M. (2020) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2020.116025 · Cited by 90 · see citing works
Switchable hydrophilicity solvents in analytical chemistry. Five years of achievements
Bazel, Y., Rečlo, M., & Chubirka, Y. (2020) · Microchemical Journal · doi:10.1016/j.microc.2020.105115 · Cited by 72 · see citing works
Zhang, M., Zhang, Z., Gul, Z., Tian, M., Wang, J., Zheng, K., Zhao, C., & Li, C. (2023) · Journal of Separation Science · doi:10.1002/jssc.202300098 · Cited by 61 · see citing works
Ullah, N., & Tüzen, M. (2023) · Green Chemistry · doi:10.1039/d3gc00020f · Cited by 56 · see citing works
Ullah, N., Haseeb, A., & Tüzen, M. (2023) · Critical Reviews in Analytical Chemistry · doi:10.1080/10408347.2023.2197495 · Cited by 56 · see citing works
Wang, Y., Yang, L. C., Xing, R., Wang, R., Chen, X., & Hu, S. (2022) · Journal of Chromatography A · doi:10.1016/j.chroma.2022.463712 · Cited by 32 · see citing works
Innovative Application of Metabolomics on Bioactive Ingredients of Foods
Hu, S., Liu, C., & Liu, X. (2022) · Foods · doi:10.3390/foods11192974 · Cited by 23 · see citing works
Scheid, C., Eller, S. C. W. D. S., Oenning, A. L., Carasek, E., Merib, J. D. O., & de Oliveira, T. F. (2021) · Journal of Analytical Toxicology · doi:10.1093/jat/bkab100 · Cited by 19 · see citing works
Shah, S. N., Erbaş, Z., & Soylak, M. (2020) · International Journal of Environmental & Analytical Chemistry · doi:10.1080/03067319.2020.1768249 · Cited by 17 · see citing works
A Review of Bioanalytical Applications of Microextraction Techniques Combined with Derivatization
Manousi, N., Ntorkou, M., Tzanavaras, P. D., & Zacharis, C. K. (2023) · Bioanalysis · doi:10.4155/bio-2023-0121 · Cited by 9 · see citing works
Yu, S., Chen, Z.-W., Wang, Y., Sheng, C., Zhang, H.-R., & Jiang, K. (2021) · Journal of Separation Science · doi:10.1002/jssc.202100218 · Cited by 9 · see citing works
Developments and trends in mycotoxin analysis: A review with bibliometric research
Wang, C., Huang, Y., Wang, Y., Zhang, J., Guo, M., Chen, C., Zhang, H., Yue, B., Kong, D., Luo, J., et al. (2024) · Microchemical Journal · doi:10.1016/j.microc.2024.111774 · Cited by 7 · see citing works
Bazeľ, Y., & Vishnikin, A. (2025) · Microchemical Journal · doi:10.1016/j.microc.2025.114868 · Cited by 4 · see citing works
Advancements and innovations in solvent-based extraction techniques
Lanjwani, M. F., Khuhawar, M. Y., Tüzen, M., Fesliyan, S., & Altunay, N. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-16122-3.00009-3 · Cited by 4 · see citing works
Wang, E., Zhang, Y., Li, C., & Qu, T. (2025) · Analytical Methods · doi:10.1039/d5ay01212k · Cited by 2 · see citing works
Chormey, D. S., Bodur, S. E., Er, E. Ö., Zaman, B. T., Bodur, S., Şaylan, M., Bozyiğit, G. D., Serbest, H., & Bakırdere, S. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-15978-7.00077-1 · Cited by 2 · see citing works
Self-citationLopez-Ruiz, I., Lasarte‐Aragonés, G., Lucena, R., & Cárdenas, S. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-822139-6.00004-3 · Cited by 1 · see citing works
- Authors
- Erarpat, S., Bodur, S., Öztürk Er, E., & Bakırdere, S.
- Affiliations
- Yıldız Technical University (TR)
- Department of Chemical Engineering Yıldız Technical University İstanbul Turkey
- Corresponding author
- Bakırdere, Sezgin
- Venue
- Journal of Separation Science
- Publisher
- Wiley
- Published
- 2020-01-08
- Volume
- 43
- Issue
- 6
- Pages
- 1100-1106
- ISSN
- 1615-9306 (ISSN-L)
- 1615-9314
- OpenAlex
- W2995428254
- Cited by
- 18
- References
- 48
- Citations by year
- 2025: 2 · 2024: 3 · 2023: 4 · 2022: 2 · 2021: 4 · 2020: 3
- FWCI
- 1.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Separation Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical chemistry methods development
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Keywords
- methionine, human plasma, ethyl chloroformate derivatization, N,N-dimethylbenzylamine, derivatization optimization, preconcentration, green analytical method, amino acid analysis
- MeSH terms
- Ultrasonic Waves, Amines, Formic Acid Esters, Humans, Gas Chromatography-Mass Spectrometry, Methionine, Solvents, Molecular Structure, Liquid Phase Microextraction, Healthy Volunteers
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
