Expression of two actin genes during larval development in the sea urchin Strongylocentrotus purpuratus
Abstract
We report the first measurements of cell number, total RNA, and transcript accumulations for two actin genes during larval development of the sea urchin Strongylocentrotus purpuratus. At 5 weeks of feeding, when development of laboratory-raised larvae is completed, the cell number has increased about 100-fold with respect to the pluteus-stage embryo to about 150,000 +/- 50,000, and the total RNA has increased 46-fold to about 130 ng per larva. The transcripts of the Cylla cytoskeletal actin gene, which is expressed in adult tissues, continue to accumulate throughout larval development. A contrasting pattern of transcript accumulation is observed for Cyllla, a different cytoskeletal actin gene that in the embryo is expressed only in aboral ectoderm. These transcripts increase in number early in larval development, when the larval epidermis is differentiating, and then decline in quantity. It is known that at metamorphosis the larval epidermis is largely histolyzed and that the Cyllla gene is not expressed in the juvenile or adult.
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Control titrations for SpZ12-1 and CyIIIa mRNAs gave values close to those previously published.
“As controls for the measurement procedures and calculations, titrations were carried out for mRNAs of the transcription factor gene SpZ12–1 (25) and of the cytoskeletal actin CyIIIa (26, 27), the same RNAs as used for estimation of Hox gene transcripts. The values observed were close to those previously published.”
From Expression of the <i>Hox</i> gene complex in the indirect development of a sea urchin (1998) · cites this paper as [26]
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- Authors
- Cameron, R. A., Britten, R. J., & Davidson, E. H.
- Affiliations
- California Institute of Technology (US)
- Corresponding author
- Cameron, R. Andrew
- ORCID
- Cameron, R. Andrew
- Venue
- Molecular Reproduction and Development
- Publisher
- Wiley
- Published
- 1989-01
- Volume
- 1
- Issue
- 3
- Pages
- 149-155
- ISSN
- 1040-452X (ISSN-L)
- 1098-2795
- OpenAlex
- W1986122733
- Cited by
- 28
- References
- 21
- Citations by year
- 2021: 2 · 2013: 1 · 2012: 1
- FWCI
- 3.99
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Reproduction and Development (publishedVersion)
- PubMed (publishedVersion)
- CaltechAUTHORS (California Institute of Technology) (submittedVersion)
- Funders
- National Institutes of Health
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD05753)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Echinoderm biology and ecology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Physical Sciences › Earth and Planetary Sciences › Paleontology › Marine Invertebrate Physiology and Ecology
- Keywords
- actin gene, Strongylocentrotus purpuratus, larval development, transcript accumulation, cell number, total RNA, cytoskeletal actin, aboral ectoderm, metamorphosis, gene expression, sea urchin embryo
- MeSH terms
- Actins, Animals, Cell Count, Cytoskeleton, Larva, RNA, Sea Urchins, Transcription, Genetic, Gene Expression
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