Cited by 24Reproductive System and PregnancyPregnancy and preeclampsia studiesReproductive Biology and Fertility

Histamine enhances cytotrophoblast invasion by inducing intracellular calcium transients through the histamine type-1 receptor.

Liu, Zitao, Kilburn, Brian A., Leach, Richard E., Romero, Roberto, Paria, Bibhash C., Armant, D. Randall·2004·Molecular Reproduction and Development

Abstract

Blastocyst implantation and placentation require molecular and cellular interactions between the uterine endometrium and blastocyst trophectoderm. Previous studies showed that histamine produced in the mouse uterine luminal epithelium interacts with trophoblast histamine type-2 receptors (H2) to initiate blastocyst implantation. However, it is unknown whether similar histamine activity is operative in humans. Using a human cell line (HTR-8/SVneo) derived from first-trimester cytotrophoblasts that expresses both histamine type-1 receptor (H1) and H2, we found that histamine promotes cytotrophoblast invasiveness specifically through activation of H1. Stimulation of H1 in human cytotrophoblasts by histamine induced intracellular Ca2+ (Ca(2+)i) transients by activating phospholipase C and the inositol trisphosphate pathway. The enhanced invasion induced by histamine was blocked by pretreatment with H1 antagonist or by chelation of Ca(2+)i. These findings suggest possible differences between rodents and humans in histamine signaling to the trophoblast.

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Crossref · 10.1002/mrd.20082

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Authors
Liu, Z., Kilburn, B. A., Leach, R. E., Romero, R., Paria, B. C., & Armant, D. R.
Affiliations
  • Wayne State University (US)
  • United States Department of Health and Human Services (US)
  • Vanderbilt University Medical Center (US)
Corresponding author
Armant, D. Randall
Venue
Molecular Reproduction and Development
Publisher
Wiley
Published
2004-04-21
Volume
68
Issue
3
Pages
345-353
ISSN
  • 1040-452X (ISSN-L)
  • 1098-2795
OpenAlex
W2079111092
Cited by
24
References
44
Citations by year
2026: 2 · 2025: 1 · 2022: 2 · 2019: 2 · 2017: 1 · 2015: 1 · 2014: 1 · 2013: 1 · 2012: 3
FWCI
0.24
Type
journal article
Language
en
Open access
closed
Locations
  • Molecular Reproduction and Development (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (HD 037394, HD36764, AA12057, HD98004, HD37500)
  • Perinatology Research Branch, Intramural Division, National Institute of Child Health and Human Development (National Institutes of Health
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
  • Health Sciences › Medicine › Obstetrics and Gynecology › Pregnancy and preeclampsia studies
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
Keywords
histamine, cytotrophoblast invasion, calcium transients, phospholipase C, HTR-8/SVneo cells, embryo implantation, placentation, endometrium, trophoblast
MeSH terms
Calcium, Cell Adhesion, Cells, Cultured, Egtazic Acid, Histamine, Histamine H1 Antagonists, Humans, Isoquinolines, Embryo Implantation, Phosphatidylinositols, Type C Phospholipases, Receptors, Histamine H1, Receptors, Histamine H2, Sulfonamides, Trophoblasts, Calcium Signaling, Cell Proliferation

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