Cited by 143Sperm and Testicular FunctionReproductive Biology and FertilityReproductive biology and impacts on aquatic species

Izumo is part of a multiprotein family whose members form large complexes on mammalian sperm

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Ellerman, Diego A., Pei, Jimin, Gupta, Surabhi, Snell, William J., Myles, Diana, Primakoff, Paul·2009·Molecular Reproduction and Development

Abstract

Izumo, a sperm membrane protein, is essential for gamete fusion in the mouse. It has an Immunoglobulin (Ig) domain and an N‐terminal domain for which neither the functions nor homologous sequences are known. In the present work we identified three novel proteins showing an N‐terminal domain with significant homology to the N‐terminal domain of Izumo. We named this region “Izumo domain,” and the novel proteins “Izumo 2,” “Izumo 3,” and “Izumo 4,” retaining “Izumo 1” for the first described member of the family. Izumo 1–3 are transmembrane proteins expressed specifically in the testis, and Izumo 4 is a soluble protein expressed in the testis and in other tissues. Electrophoresis under mildly denaturing conditions, followed by Western blot analysis, showed that Izumo 1, 3, and 4 formed protein complexes on sperm, Izumo 1 forming several larger complexes and Izumo 3 and 4 forming a single larger complex. Studies using different recombinant Izumo constructs suggested the Izumo domain possesses the ability to form dimers, whereas the transmembrane domain or the cytoplasmic domain or both of Izumo 1 are required for the formation of multimers of higher order. Co‐immunoprecipitation studies showed the presence of other sperm proteins associated with Izumo 1, suggesting Izumo 1 forms a multiprotein membrane complex. Our results raise the possibility that Izumo 1 might be involved in organizing or stabilizing a multiprotein complex essential for the function of the membrane fusion machinery. Mol. Reprod. Dev. 76: 1188–1199, 2009. © 2009 Wiley‐Liss, Inc.

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  • A splice site change in IZUMO4 is a possible SNC that might be relevant, since proteins encoded by the IZUMO family form complexes on mammalian sperm.

    “Another possible SNC that might be relevant in this context is a splice site change in IZUMO4, since proteins encoded by the IZUMO family form complexes on mammalian sperm175.”

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  1. Stein KK Go JC Lane WS Primakoff P Myles DG Proteomic analysis of sperm regions that mediate sperm-egg interactions Proteomics 2006 6 12 3533 3543 16758446

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Authors
Ellerman, D. A., Pei, J., Gupta, S., Snell, W. J., Myles, D., & Primakoff, P.
Affiliations
  • University of California, Davis (US)
  • Howard Hughes Medical Institute (US)
  • The University of Texas Southwestern Medical Center (US)
Corresponding author
Ellerman, Diego A.
Venue
Molecular Reproduction and Development
Publisher
Wiley
Published
2009-08-05
Volume
76
Issue
12
Pages
1188-1199
ISSN
  • 1040-452X (ISSN-L)
  • 1098-2795
OpenAlex
W2063034367
Cited by
143
References
27
Citations by year
2026: 4 · 2025: 7 · 2024: 9 · 2023: 15 · 2022: 13 · 2021: 5 · 2020: 9 · 2019: 9 · 2018: 3 · 2017: 4 · 2016: 12 · 2015: 10 · 2014: 11 · 2013: 5 · 2012: 8
FWCI
10.60
Type
journal article
Language
en
Open access
Locations
  • Molecular Reproduction and Development (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institutes of Health
  • U.S. Public Health Service (U54 29125, 16580)
  • National Institute of General Medical Sciences (GM-56778, R01GM056778)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Reproductive biology and impacts on aquatic species
Keywords
Izumo, sperm membrane proteins, gamete fusion, immunoglobulin domains, N-terminal domain, protein complexes, protein dimerization, protein oligomerization, testis-specific proteins
MeSH terms
Amino Acid Sequence, Animals, Cricetulus, Cricetinae, Humans, Immunoglobulins, Male, Membrane Proteins, Molecular Sequence Data, Recombinant Fusion Proteins, Spermatozoa, Sequence Alignment, CHO Cells, Protein Isoforms, Multiprotein Complexes, Mice, Protein Multimerization

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