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Brief, high‐temperature heat denaturation (pressure cooking): A simple and effective method of antigen retrieval for routinely processed tissues
Abstract
The merits of microwave‐based antigen retrieval methods for diagnostic immunohistochemistry have recently been emphasized. As such technology has drawbacks for busy routine laboratories, we have investigated an alternative method of heat‐mediated antigen retrieval (HMAR), using a domestic pressure cooker as a source of superheating. A simple protocol, with superheating in 0.01 M citrate buffer (pH 6.0) for 2 min, gave results comparable to those obtainable in a microwave oven. The range of antigens retrieved and the antigens for which heating was ineffective matched the published microwave experience. The advantages included the speed of treatment, the reproducibility of results with large batches of slides, the ability to use metal slide racks, and economy of time and equipment costs.
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Citations
Cited by
Validation of Tissue Microarray Technology in Breast Carcinoma
Camp, R. L., Charette, L. A., & Rimm, D. L. (2000) · Laboratory Investigation · doi:10.1038/labinvest.3780204 · Cited by 799
Antigen Retrieval Immunohistochemistry: Past, Present, and Future
Shi, S.-R., Cote, R. J., & Taylor, C. R. (1997) · Journal of Histochemistry & Cytochemistry · doi:10.1177/002215549704500301 · Cited by 516
Sabattini, E., Bisgaard, K., Ascani, S., Poggi, S., Piccioli, M., Ceccarelli, C., Pieri, F., Fraternali-Orcioni, G., & Pileri, S. A. (1998) · Journal of Clinical Pathology · doi:10.1136/jcp.51.7.506 · Cited by 461
Antigen retrieval techniques in immunohistochemistry: comparison of different methods
Pileri, S. A., Roncador, G., Ceccarelli, C., Piccioli, M., Briskomatis, A., Sabattini, E., Ascani, S., Santini, D., Piccaluga, P. P., Leone, O., et al. (1997) · The Journal of Pathology · doi:10.1002/(sici)1096-9896(199709)183:1<116::aid-path1087>3.0.co;2-2 · Cited by 338
Kaufmann, O., Fietze, E., Mengs, J., & Dietel, M. (2001) · American Journal of Clinical Pathology · doi:10.1309/21tw-2ndg-jrk4-pfjx · Cited by 324
Metadata
- Authors
- Norton, A. J., Jordan, S., & Yeomans, P.
- Venue
- The Journal of Pathology
- Publisher
- Wiley
- Published
- 1994-08
- Volume
- 173
- Issue
- 4
- Pages
- 371-379
- ISSN
- 0022-3417 (ISSN-L)
- 1096-9896
- OpenAlex
- W2065411915
- Cited by
- 471
- References
- 0
- FWCI
- 24.51
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Brief, high‐temperature heat denaturation (pressure cooking): A simple and effective method of antigen retrieval for routinely processed tissues. https://app.cochat.ai/papers/10.1002/path.1711730413
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- Norton, A. J., Jordan, S., & Yeomans, P. (1994). Brief, high‐temperature heat denaturation (pressure cooking): A simple and effective method of antigen retrieval for routinely processed tissues. The Journal of Pathology. https://doi.org/10.1002/path.1711730413
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
