Capillary zone electrophoresis for bottom‐up analysis of complex proteomes
Open accessAbstract
Capillary zone electrophoresis (CZE) is emerging as a useful tool in proteomic analysis. Interest arises from dramatic improvements in performance that result from improvements in the background electrolyte used for the separation, the incorporation of advanced sample injection methods, the development of robust and sensitive electrospray interfaces, and the coupling with Orbitrap mass spectrometers with high resolution and sensitivity. The combination of these technologies produces performance that is rapidly approaching the performance of UPLC‐based methods for microgram samples and exceeds the performance of UPLC‐based methods for mid‐ to low nanogram samples. These systems now produce over 10 000 peptide IDs in a single 100‐min analysis of the HeLa proteome.
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In dynamic pH junction, a focused plug forms and target analytes become positively charged and migrate toward the ESI source.
“A focused plug is formed and target analytes become positively charged, migrating towards the ESI source25,118.”
From Recent trends of capillary electrophoresis‐mass spectrometry in proteomics research (2019) · cites this paper as [25]
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References 41 · Cited by 41
- Authors
- Sun, L., Zhu, G., Yan, X., Zhang, Z., Wojcik, R., Champion, M. M., & Dovichi, N. J.
- Affiliations
- University of Notre Dame (US)
- Department of Chemistry and Biochemistry University of Notre Dame Notre Dame IN USA
- Venue
- PROTEOMICS
- Publisher
- Wiley
- Published
- 2015-12-20
- Volume
- 16
- Issue
- 2
- Pages
- 188-196
- ISSN
- 1615-9853 (ISSN-L)
- 1615-9861
- PMC
- PMC4771184
- OpenAlex
- W2098988563
- Cited by
- 41
- References
- 41
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 5 · 2019: 3 · 2018: 7 · 2017: 7 · 2016: 7 · 2013: 1
- FWCI
- 0.67
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- PROTEOMICS (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (R01GM096767)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Keywords
- capillary zone electrophoresis, shotgun proteomics, supporting electrolyte, Orbitrap mass spectrometry, peptide identification
- MeSH terms
- Humans, Electrophoresis, Capillary, Proteome, Spectrometry, Mass, Electrospray Ionization, Proteomics, Quality Improvement
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