Single‐cell pluripotency regulatory networks
Open accessPublished version of a preprint
Abstract
Pluripotent stem cells (PSCs) are a popular model system for investigating development, tissue regeneration, and repair. Although much is known about the molecular mechanisms that regulate the balance between self‐renewal and lineage commitment in PSCs, the spatiotemporal integration of responsive signaling pathways with core transcriptional regulatory networks are complex and only partially understood. Moreover, measurements made on populations of cells reveal only average properties of the underlying regulatory networks, obscuring their fine detail. Here, we discuss the reconstruction of regulatory networks in individual cells using novel single‐cell transcriptomics and proteomics, in order to expand our understanding of the molecular basis of pluripotency, including the role of cell–cell variability within PSC populations, and ways in which networks may be controlled in order to reliably manipulate cell behavior.
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Cites
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- Authors
- Stumpf, P. S., Ewing, R., & MacArthur, B. D.
- Affiliations
- University of Southampton (GB)
- Corresponding author
- Ewing, Rob
- MacArthur, Ben D.
- Venue
- PROTEOMICS
- Publisher
- Wiley
- Published
- 2016-08-12
- Volume
- 16
- Issue
- 17
- Pages
- 2303-2312
- ISSN
- 1615-9853 (ISSN-L)
- 1615-9861
- OpenAlex
- W2460035027
- Cited by
- 4
- References
- 9
- Citations by year
- 2019: 1 · 2018: 1 · 2017: 2
- FWCI
- 0.69
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Licence
- Locations
- PROTEOMICS (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- ePrints Soton (University of Southampton) (submittedVersion)
- Funders
- Seventh Framework Programme (Oncoprotnet / FP7-PEOPLE-2012-CIG)
- Biotechnology and Biological Sciences Research Council (BB/L000512)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
- Keywords
- pluripotency, pluripotent stem cells, transcriptional regulatory networks, single-cell transcriptomics, single-cell proteomics, self-renewal, lineage commitment, signaling pathways, network inference
- MeSH terms
- Animals, Cell Differentiation, Humans, Signal Transduction, Pluripotent Stem Cells, Proteomics, Gene Regulatory Networks, Metabolic Networks and Pathways, Transcriptome, Protein Interaction Maps, Cellular Reprogramming
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