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The determination of protein, oligonucleotide and peptide molecular weights by ion‐spray mass spectrometry
Abstract
The mass spectra of several compounds with molecular weights in the 2500–20 000 Da range were obtained with a quadrupole mass spectrometer equipped with an atmospheric pressure ion source. Average molecular weight determinations of mellitin (2846.4 Da), a synthetic oligonucleotide (4262.8 Da), myoglobin (16 950.4 Da) and on the subunits of β‐lactoglobulin (18 277.1 Da) requiring as little as 1 pmol of material were achieved with accuracies and precisions of ± 1 Da. An ion‐spray interface was used to produce ions via the ion evaporation process, producing mass spectra containing a series of multiply‐charged molecular species. A simple method for calculating the molecular weight of unknown compounds from the spectra containing multiply‐charged ions is described.
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Citations
Cited by
Electrospray Ionization for Mass Spectrometry of Large Biomolecules
Fenn, J. B., Mann, M., Meng, C. K., Wong, S. F., & Whitehouse, C. M. (1989) · Science · doi:10.1126/science.2675315 · Cited by 7,634
New developments in biochemical mass spectrometry: electrospray ionization
Smith, R. D., Loo, J. A., Edmonds, C. G., Barinaga, C. J., & Udseth, H. R. (1990) · Analytical Chemistry · doi:10.1021/ac00208a002 · Cited by 1,051
[21] Electrospray ionization mass spectrometry
Fred Banks, J., & Whitehouse, C. M. (1996) · Methods in Enzymology · doi:10.1016/s0076-6879(96)70023-x · Cited by 522
Covey, T. R., Huang, E. C., & Henion, J. D. (1991) · Analytical Chemistry · doi:10.1021/ac00013a003 · Cited by 446
Self-citationRapid, sensitive analysis of protein mixtures by mass spectrometry.
Beavis, R. C., & Chait, B. T. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.17.6873 · Cited by 301
Metadata
- Authors
- Covey, T. R., Bonner, R. F., Shushan, B. I., Henion, J., & Boyd, R. K.
- Venue
- Rapid Communications in Mass Spectrometry
- Publisher
- Wiley
- Published
- 1988-11
- Volume
- 2
- Issue
- 11
- Pages
- 249-256
- ISSN
- 0951-4198 (ISSN-L)
- 1097-0231
- OpenAlex
- W1973486893
- Cited by
- 593
- References
- 0
- FWCI
- 32.17
- Type
- journal article
- Language
- en
- Open access
- closed
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- Covey, T. R., Bonner, R. F., Shushan, B. I., Henion, J., & Boyd, R. K. (1988). The determination of protein, oligonucleotide and peptide molecular weights by ion‐spray mass spectrometry. Rapid Communications in Mass Spectrometry. https://doi.org/10.1002/rcm.1290021111
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