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Solid‐phase permethylation of glycans for mass spectrometric analysis
Open accessAbstract
A miniaturized approach was developed for quantitative permethylation of oligosaccharides, which involves packing of sodium hydroxide powder in microspin columns or fused‐silica capillaries (500 µm i.d.), permitting effective derivatization in less than a minute at microscale. Prior to mass spectrometry, analytes are mixed with methyl iodide in dimethyl sulfoxide solution containing traces of water before infusing through the microreactors. This procedure minimizes oxidative degradation and peeling reactions and avoids the need of excessive clean‐up. Picomole amounts of linear and branched, sialylated and neutral glycan samples were rapidly and efficiently permethylated by this approach and analyzed by matrix‐assisted laser desorption/ionization mass spectrometry. Copyright © 2005 John Wiley & Sons, Ltd.
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Cited by
Mass spectrometry approaches to glycomic and glycoproteomic analyses
Ruhaak, L. R., Xu, G., Li, Q., Goonatilleke, E., & Lebrilla, C. B. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00732 · Cited by 395
Reiding, K. R., Blank, D., Kuijper, D. M., Deelder, A. M., & Wuhrer, M. (2014) · Analytical Chemistry · doi:10.1021/ac500335t · Cited by 334
High-sensitivity Analytical Approaches for the Structural Characterization of Glycoproteins
Alley, W. R., Mann, B. F., & Novotny, M. V. (2013) · Chemical Reviews · doi:10.1021/cr3003714 · Cited by 310
Self-citationHarvey, D. J. (2011) · Journal of Chromatography B · doi:10.1016/j.jchromb.2010.11.010 · Cited by 260
Mass spectrometric analysis of N- and O-glycosylation of tissues and cells
HASLAM, S., NORTH, S., & DELL, A. (2006) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2006.08.006 · Cited by 111
Metadata
- Authors
- Kang, P., Mechref, Y., Klouckova, I., & Novotny, M. V.
- Venue
- Rapid Communications in Mass Spectrometry
- Publisher
- Wiley
- Published
- 2005-10-27
- Volume
- 19
- Issue
- 23
- Pages
- 3421-3428
- ISSN
- 0951-4198 (ISSN-L)
- 1097-0231
- OpenAlex
- W2089294127
- Cited by
- 322
- References
- 0
- FWCI
- 4.84
- Type
- journal article
- Language
- en
- Open access
- green
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- Kang, P., Mechref, Y., Klouckova, I., & Novotny, M. V. (2005). Solid‐phase permethylation of glycans for mass spectrometric analysis. Rapid Communications in Mass Spectrometry. https://doi.org/10.1002/rcm.2210
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