The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Simple Synthesis of Hierarchically Ordered Mesocellular Mesoporous Silica Materials Hosting Crosslinked Enzyme Aggregates
Abstract
H ierarchically ordered mesocellular mesoporous silica materials (HMMS) were synthesized using a single structure‐directing agent. The mesocellular pores are synthesized without adding any pore expander; the pore walls are composed of SBA‐15 type mesopores. Small‐angle X‐ray scattering revealed the presence of uniform pore structures with two different sizes. Using HMMS as a nanoscopic template, hierarchically ordered mesocellular mesoporous carbon (HMMC) and polymer (HMMP) materials were synthesized. HMMS was used as a host for enzyme immobilization. To improve the retention of enzymes in HMMS, we adsorbed enzymes, and then employed crosslinking using glutaraldehyde (GA). The resulting crosslinked enzyme aggregates (CLEAs) show an impressive stability with extremely high enzyme loadings. For example, 0.5 g α‐chymotrypsin (CT) could be loaded in 1 g of silica with no activity decrease observed with rigorous shaking over one month. In contrast, adsorbed CT without GA treatment resulted in a lower loading, which further decreased due to continuous leaching of adsorbed CT under shaking. The activity of crosslinked CT aggregates in HMMS was ≈10 times higher than that of the adsorbed CT, which represents a 74‐fold increase in activity per unit weight of HMMS due to higher CT loading.
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Cites
Measurement of protein using bicinchoninic acid
Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., & Klenk, D. C. (1985) · Analytical Biochemistry · doi:10.1016/0003-2697(85)90442-7 · Cited by 18,482
Ordered porous materials for emerging applications
Davis, M. E. (2002) · Nature · doi:10.1038/nature00785 · Cited by 5,308
Characterization of the porous structure of sba-15
Kruk, M., Jaroniec, M., Ko, C. H., & Ryoo, R. (2000) · Chemistry of Materials · doi:10.1021/cm000164e · Cited by 1,409
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Mesoporous carbon materials: Synthesis and modification
Liang, C., Li, Z., & Dai, S. (2008) · Angewandte Chemie International Edition · doi:10.1002/anie.200702046 · Cited by 1,935
Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance
Garcia‐Galan, C., Berenguer‐Murcia, Á., Fernandez‐Lafuente, R., & Rodrigues, R. C. (2011) · Advanced Synthesis & Catalysis · doi:10.1002/adsc.201100534 · Cited by 1,614
Enzyme immobilization: an update
Homaei, A. A., Sariri, R., Vianello, F., & Stevanato, R. (2013) · Journal of Chemical Biology · doi:10.1007/s12154-013-0102-9 · Cited by 875
Proteins in Mesoporous Silicates
Hudson, S., Cooney, J., & Magner, E. (2008) · Angewandte Chemie International Edition · doi:10.1002/anie.200705238 · Cited by 663
Novozym 435: the “perfect” lipase immobilized biocatalyst?
Ortiz, C., Ferreira, M. L., Barbosa, O., dos Santos, J. C. S., Rodrigues, R. C., Berenguer-Murcia, Á., Briand, L. E., & Fernandez-Lafuente, R. (2019) · Catalysis Science & Technology · doi:10.1039/c9cy00415g · Cited by 628
Metadata
- Authors
- Lee, J., Kim, J., Kim, J., Jia, H., Kim, M. I., Kwak, J. H., Jin, S., Dohnalkova, A., Park, H. G., Chang, H. N., Wang, P., Grate, J. W., & Hyeon, T.
- Venue
- Small
- Publisher
- Wiley
- Published
- 2005-06-08
- Volume
- 1
- Issue
- 7
- Pages
- 744-753
- ISSN
- 1613-6810 (ISSN-L)
- 1613-6829
- OpenAlex
- W2059102380
- Cited by
- 187
- References
- 3
- FWCI
- 7.46
- Type
- journal article
- Language
- en
- Open access
- closed
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- Lee, J., Kim, J., Kim, J., Jia, H., Kim, M. I., Kwak, J. H., Jin, S., Dohnalkova, A., Park, H. G., Chang, H. N., Wang, P., Grate, J. W., & Hyeon, T. (2005). Simple Synthesis of Hierarchically Ordered Mesocellular Mesoporous Silica Materials Hosting Crosslinked Enzyme Aggregates. Small. https://doi.org/10.1002/smll.200500035
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